한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제34권4호
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  • Pages.12-23
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  • 2021
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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내소황련탕(內疎黃連湯)의 항노화 및 항산화 효능 연구

Anti-aging and Anti-oxidative Effect of Naesohwangryun-tang(NSHRT)

  • 오솔라 (세명대학교 화장품과학과) ;
  • 남상우 (세명대학교 화장품뷰티생명공학부) ;
  • 송영채 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김용민 (세명대학교 화장품뷰티생명공학부) ;
  • 이천일 (세명대학교 화장품뷰티생명공학부)
  • Oh, Sol-Ra (Department Of Cosmetic Science, Semyung University) ;
  • Nam, Sang-Woo (Department Of Cosmetic Sciences & Beauty Biotechnology, Semyung University) ;
  • Song, Young-Chae (Department Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Hee-Taek (Department Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Yong-Min (Department Of Cosmetic Sciences & Beauty Biotechnology, Semyung University) ;
  • Lee, Chun-Il (Department Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
  • 투고 : 2021.10.01
  • 심사 : 2021.11.09
  • 발행 : 2021.11.25
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초록

Objectives : This study is to investigate the anti-aging and antioxidant effects of Naesohwangryeon-tang(NSHRT) extract in Hs68 fibroblasts. Methods : The fibroblast cell line Hs68 cells were used and MTT analysis was performed to measure the cell viability of Naesohwangryeon-tang, and then MMP-1 was induced with the cytokine TNF-𝛼 to confirm the anti-aging effect through real-time PCR and ELISA. In addition, ROS was induced with the TNF-𝛼 to confirm the anti-oxidant effect through DCF-DA. The signaling pathway according to the antioxidant effect was performed by western blot. Results : Naesohwangryeon-tang did not show cytotoxicity at all concentrations(100-800㎍/㎖) treated on Hs68 cells, and the inhibitory effect of MMP-1 was also confirmed. In addition, it was confirmed that the intracellular ROS increased by TNF-α was decreased by Naesohwangryeon-tang, and it was confirmed that the expression of NQO1 was increased in a concentration-dependent manner of Naesohwangryeon-tang. Conclusions : These results suggest that Naesohwangryeon-tang can be used as an anti-aging material by demonstrating the anti-aging and antioxidant effects of Naesohwangryeon-tang.

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과제정보

본 연구는 2021년도 세명대학교 대학혁신 지원사업에 의해 수행되었습니다.

참고문헌

  1. Hong SH, Lee HN, Jeong TK, Kim YH, Jeong HS, Lee JS. Protective Effect of Jujube Water Extract against UVB-Induced Skin Photoaging in Human Skin Fibroblasts. J Korean Soc Food Sci Nutr. 2019;48(10):1172-7. https://doi.org/10.3746/jkfn.2019.48.10.1172
  2. Jeong HC, Kim KM, Lee SH, Kim YH, Lee SY, Choi DH, et al. Inhibitory Effect of Novel Lactobacillus paracasei SKB1192 on Skin Photoaging. J Kor Soc Cosmetol. 2019;25(3):591-9.
  3. Gwon GY, Park GG. Protective Effects of Bifidobacterium bifidum Culture Supernatants and Intracellular Cell-Free Extracts on Human Dermal Fibroblasts against UV-B Irradiation. J Korean Soc Food Sci Nutr. 2017;46(7):801-8. https://doi.org/10.3746/JKFN.2017.46.7.801
  4. Nam JJ, Kim YJ. Fractionated Trapa japonica Extracts Inhibit ROS-induced Skin Inflammation in HaCaT keratinocytes. J. Soc. Cosmet. Sci. Korea. 2015;41(1):45-55. https://doi.org/10.15230/SCSK.2015.41.1.45
  5. An SS, Heo DS. Effects of Kyejiinsam-tang in MIA-Induced Osteoarthritis Rats. J Korean Med. 2013;34(3):69-85. https://doi.org/10.13048/jkm.13013
  6. Kim JE, Choi YS, Kim HK, Jang YA. Collagen synthesis ability and inhibitory effect of MMPs in keratinocytes of Lysimachia christinae Hance Extract. Journal of the Korean Applied Science and Technology. 2020;37(4):820-9. https://doi.org/10.12925/JKOCS.2020.37.4.820
  7. Na EJ, Yang HO, Choi YE, Han HS, Rhie SJ, Ryu JY. Anti-inflammatory and Collagen Production Effect of Syringic Acid on Human Keratinocyte (HaCaT) Damaged by Ultraviolet B. Asian J Beauty Cosmetol. 2018;16(4):523-31. https://doi.org/10.20402/ajbc.2018.0245
  8. Park SM, Kim JJ, Jeong KY, Han SK, Jeong TH, Yun MY. Antioxidant Activity and Inhibition of MMP-1 Expression of Schizandrae fructus (Schizandra chinensis) Extract. Kor. J. Pharmacogn. 2013;44(1):47-52.
  9. Robert V, Hideaki N. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circulation research. 2003;92:827-39. https://doi.org/10.1161/01.RES.0000070112.80711.3D
  10. Kwak CS, Yang JW. Prevention Effect of Prunus persica Flos Extract from Reactive Oxygen Species Generation and Matrix Metalloproteinases Production Induced by UVB Irradiation in Human Skin Cells. Asian J Beauty Cosmetol. 2016;14(2):179-90. https://doi.org/10.20402/ajbc.2016.0043
  11. Heo J. Donguibogam. Hadong: Donguibogam Publisher. 2006:1539-86.
  12. Ko WS, Kim KJ, Kim NK, Kim YB, Kim JH, Kim HT, et al. Text of Traditional Korean Dermatology & Surgery. 1st ed. Busan: Seonu. 2007:28-32.
  13. Ahn JH, Sim SY, Kim KJ. The Anti-Inflammatory Effects of NeiShuHuangLianTang In Experi-mental Animals. The J of Korean Oriental Medical Ophthalmology Otolaryngology Dermatology 2004;17(3):8-17.
  14. Jeon SH, Kim TJ, Kim YM. Anti-inflammatory mechanism and Anti-oxidant Effects of Naesohwangryun-tang in LPS-Stimulated RAW 264.7 Macrophage Cells. J Korean Med Ophthalmol Otolaryngol Dermatol 2020;33(2):100-11. https://doi.org/10.6114/JKOOD.2020.33.2.100
  15. Lee JH, Lee SN, Kim MG, Kim MH, Kim HJ, Jo HJ, Leem KH. Effects of Angelica Gigantis Radix Extracts on the Collagenase Activity and Procollagen synthesis in HS68 Human Fibroblasts and Tyrosinase Activity. Korean J. Herbology. 2011;26(1):29-33.
  16. Kim MJ, Kim JY, Jung TK, Choi SW, Yoon KS. Skin Anti-aging Effect of Forsythia viridissima L. Extract. Korean J. Biotechnol. Bioeng. 2006;21(6):444-50.
  17. Lee JN, Kim YS. The Effects of Paeonia Lactiflora Pallas on Inhibition of Oxygen Free Radical, Anti-inflammation and MMP-1 Inhibitory Activity. Journal of Oil & Applied Science. 2018;35(3):797-806. https://doi.org/10.12925/JKOCS.2018.35.3.797
  18. Park SM, Lee GW, Cho YH. Effect of Rheum undulatum Extract on Antioxidant Activity and Activity of Matrix Metalloproteinase-1 in Human Skin Fibroblasts. Journal of Life Science. 2008;18(12):1700-4. https://doi.org/10.5352/JLS.2008.18.12.1700
  19. Kang HC, Cha MY, Kim JY. A study of the Antioxidant Activities and Whitening Activities of Areca semen Extracts as Cosmetic Ingredient. J. Soc. Cosmet. Sci. Korea. 2015;41(3):269-77. https://doi.org/10.15230/SCSK.2015.41.3.269
  20. Kim SH, Jung H, Shin YC, Ko SG. Research of Traditional Herbal Medicines for Anti-aging, Inhibition Effect of Wrinkle and Whitening Effect in the Skin. Korean J. Oriental Physiology & Pathology. 2008;22(3):691-8.
  21. Kim NY, Moon JS, Choe TB. Effect of Scutellaria Baicalensis Extract Metalloproteinase-1 on the Expression of UV-B-irradidated Human Dermal Fibroblast. Korean J. Aesthet. Cosmetol. 2013;11(4):649-54.
  22. Jang YA, Lee JT. Anti-wrinkle effect of berberine by inhibition of MMP-2 and MMP-9 activity in fibroblasts. J Appl Biol Chem. 2018;61:9-15. https://doi.org/10.3839/jabc.2018.002
  23. Lee HJ, Lim MH. Biochemical Antioxidant Activity and Inhibitory Effect of Extract of Aucklandia lappa Decne against Cell Differentitation in 3T3-L1 Adipocytes. J. Kor. Soc. Cosmetol. 2018;24(1):76-84.
  24. Kim JK, Lee JH, Yang MS, Seo DB, Lee JS. Beneficial Effect of Collagen Peptide Supplement on Anti-aging Against Photodamage. Korean J. Food Sci. Technol. 2009;41(4):441-5.
  25. Kim JM, Kim SY, Noh EM, Song HK, Lee GS, Kwon KB, Lee YR. Reversine inhibits MMP-1 and MMP-3 expressions by suppressing of ROS/MAPK/AP-1 activation in UV-stimulated human keratinocytes and dermal fibroblasts. Experimental Dermatology. 2003;92:827-39.
  26. Jang YA, Lee JT. Anti-wrinkle effect of berberine by inhibition of MMP-2 and MMP-9 activity in fibroblasts. J Appl Biol Chem. 2018;61(1);9-15. https://doi.org/10.3839/jabc.2018.002
  27. Jeon JM. Choi SK, Kim YJ, Jang SJ, Cheon JW. Lee HS. Antioxidant and Antiaging Effect of Ginseng Berry Extract Fermented by Lactic Acid Bacteria. J. Soc. Cosmet. Scientists Korea. 2011;37(1):75-81. https://doi.org/10.15230/SCSK.2011.37.1.075
  28. Noh EM, Dong HK, Kim JM, Lee GS, Kwon KB, Lee YR. Extracts of Torilis Japonica Suppresses of Ultraviolet B-induced Matrix Metalloproteinase-1/-3 Expressions in Human Dermal Fibroblasts. J Physiology & Pathology Korean Med. 2019;33(3):175-80. https://doi.org/10.15188/kjopp.2019.06.33.3.175
  29. Ham SA, Kang ES, Lee HN, Hwang JS, Yoo TS, Paek KS, et al. PPARd Inhibits UVB-Induced Secretion of MMP-1 through MKP-7-Mediated Suppression of JNK Signaling. Journal of Investigative Dermatology. 2013;133:2593-600. https://doi.org/10.1038/jid.2013.202
  30. Yang JW, Kwak CS. Inhibitory effect of Aralia elata ethanol extract against skin damage in UVB-exposed human keratinocytes and human dermal fibroblasts. J Nutr Health. 2016;49(6):429-36. https://doi.org/10.4163/jnh.2016.49.6.429
  31. Lu J, Guo JH, Tu XL, Zhang C, zhao M, Zhang QW. Gao FH. Tiron Inhibits UVB-Induced AP-1 Binding Sites Transcriptional Activation on MMP-1 and MMP-3 Promoters by MAPK Signaling Pathway in Human Dermal Fibroblasts. PLoS one. 2003;92:827-39.
  32. Kim YD, Kim YM, Mo EK. Antibacterial, Antioxidant, and Antiaging Effects of the Ethanol Extract of Dolnamul (Sedum sarmentosum) and the Production of the Oil in Water Cream. J. Soc. Cosmet. Sci. Korea. 2017;43(3):211-21. https://doi.org/10.15230/SCSK.2017.43.3.211
  33. Jin KS, Lee JY, Kwon HJ, Kim BW. Anti-oxidative and Anti-inflammatory Activities of Decaisnea insignis Ethanol Extract. Jounal of Life Science. 2014;24(9):973-80. https://doi.org/10.5352/JLS.2014.24.9.973
  34. Jeon SH, Oh SL, Kim SJ, Jeon BH, Sung JY, Kim YM. Anti-oxidative Effect of Chungsimyeonja-um (CSYJE) via Nrf2/HO-1 Pathway Activity in Lipopolysaccharide (LPS) Induced RAW 264.7 Macrophages. J. Soc. Cosmet. Sci. Korea. 2020;46(3):253-63. https://doi.org/10.15230/SCSK.2020.46.3.253
  35. Kim DY. Anti-oxidative Effect of Graviola Leaf Extracts via Activation of Nrf2/HO-1 Pathway in Macrophages. J Invest Cosmetol. 2019;15(3):275-83. https://doi.org/10.15810/JIC.2019.15.3.006
  36. Lee J, Kim KS, Lee HG, Park CH, Ku MS, Keum YS. Skin Protective Effect of Methylated Marliolide through Induction of NRF2/ARE. J. Soc. Cosmet. Sci. Korea. 2018;44(4):375-9. https://doi.org/10.15230/SCSK.2018.44.4.375