Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bioconversion of Lignocellulosic Materials with the Contribution of a Multifunctional GH78 Glycoside Hydrolase from Xylaria polymorpha to Release Aromatic Fragments and Carbohydrates

  • Liers, Christiane (International Graduate School of Zittau (IHI Zittau), Dresden University of Technology) ;
  • Ullrich, Rene (International Graduate School of Zittau (IHI Zittau), Dresden University of Technology) ;
  • Kellner, Harald (International Graduate School of Zittau (IHI Zittau), Dresden University of Technology) ;
  • Chi, Do Huu (Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine) ;
  • Quynh, Dang Thu (Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology) ;
  • Luyen, Nguyen Dinh (Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology) ;
  • Huong, Le Mai (Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology) ;
  • Hofrichter, Martin (International Graduate School of Zittau (IHI Zittau), Dresden University of Technology) ;
  • Nghi, Do Huu (Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology)
  • Received : 2021.06.18
  • Accepted : 2021.08.19
  • Published : 2021.10.28
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Abstract

A bifunctional glycoside hydrolase GH78 from the ascomycete Xylaria polymorpha (XpoGH78) possesses catalytic versatility towards both glycosides and esters, which may be advantageous for the efficient degradation of the plant cell-wall complex that contains both diverse sugar residues and esterified structures. The contribution of XpoGH78 to the conversion of lignocellulosic materials without any chemical pretreatment to release the water-soluble aromatic fragments, carbohydrates, and methanol was studied. The disintegrating effect of enzymatic lignocellulose treatment can be significantly improved by using different kinds of hydrolases and phenoloxidases. The considerable changes in low (3 kDa), medium (30 kDa), and high (> 200 kDa) aromatic fragments were observed after the treatment with XpoGH78 alone or with this potent cocktail. Synergistic conversion of rape straw also resulted in a release of 17.3 mg of total carbohydrates (e.g., arabinose, galactose, glucose, mannose, xylose) per gram of substrate after incubating for 72 h. Moreover, the treatment of rape straw with XpoGH78 led to a marginal methanol release of approximately 17 ㎍/g and improved to 270 ㎍/g by cooperation with the above accessory enzymes. In the case of beech wood conversion, the combined catalysis by XpoGH78 and laccase caused an effect comparable with that of fungal strain X. polymorpha in woody cultures concerning the liberation of aromatic lignocellulose fragments.

Keywords

Acknowledgement

This research is partly funded by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) (Grant No. FWO.104.2017.03) and the Ministry of Science and Technology (NDT.45.GER/18).

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