The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Immuno-Enhancing Effects through Macrophages of Polysaccharides Isolated from Citrus Peels

진피로부터 분리한 다당의 대식세포를 통한 면역증진 효과

  • Lee, Kyung-Ae (Dept. of Food and Nutrition, Anyang University) ;
  • Park, Hye-Ryung (Graduate School of Biotechnology, and College of Life Science, Kyung Hee University)
  • 이경애 (안양대학교 식품영양학과) ;
  • 박혜령 (경희대학교 한방생명공학과)
  • Received : 2021.07.26
  • Accepted : 2021.09.06
  • Published : 2021.10.31
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Abstract

This study was designed to investigate the intracellular signaling pathways and immunoenhancing effect of macrophage activation by crude polysaccharides (CPP) extracted from citrus peels. CPP did not affect the cytotoxicity of RAW264.7 cells, but showed dose-dependent effects on cell viability. Also, CPP showed high production of chemokine (nitric oxide (NO)) and cytokines (interleukin (IL)-6 and tumor necrosis factor (TNF)-α). CPP increased IL-6, TNF-α, and inducible nitric oxide synthase (iNOS) mRNA expression dose-dependently. CPP also strongly induced the phosphorylation of the ERK, p38, and IκBα pathways in RAW 264.7 cells. In anti-pattern recognition receptors (PRRs) experiments, the effect of CPP on NO production was strongly suppressed by neutralizing toll-like receptor (TLR)2, TLR4, and Dectin1 antibodies, whereas IL-6 and TNF-α production by CPP was mainly suppressed by mannose receptor (MR). Therefore, these results suggest that CPP treatment-induced NO production was regulated by the ERK, p38, and NF-κB pathways through TLR2, TLR4, and Dectin1 receptors, whereas IL-6 and TNF-α production was primarily regulated by the ERK, p38, and NF-κB pathways through MR receptors.

Keywords

Acknowledgement

이 성과는 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구입니다(No. NRF-2019R1C1C1006496).

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