Molecules and Cells

  • 제44권10호
  • /
  • Pages.746-757
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  • 2021
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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MET1-Dependent DNA Methylation Represses Light Signaling and Influences Plant Regeneration in Arabidopsis

  • Shim, Sangrea (Department of Chemistry, Seoul National University) ;
  • Lee, Hong Gil (Plant Genomics and Breeding Institute, Seoul National University) ;
  • Seo, Pil Joon (Department of Chemistry, Seoul National University)
  • 투고 : 2021.06.18
  • 심사 : 2021.08.24
  • 발행 : 2021.10.31
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초록

Plant somatic cells can be reprogrammed into a pluripotent cell mass, called callus, which can be subsequently used for de novo shoot regeneration through a two-step in vitro tissue culture method. MET1-dependent CG methylation has been implicated in plant regeneration in Arabidopsis, because the met1-3 mutant exhibits increased shoot regeneration compared with the wild-type. To understand the role of MET1 in de novo shoot regeneration, we compared the genome-wide DNA methylomes and transcriptomes of wildtype and met1-3 callus and leaf. The CG methylation patterns were largely unchanged during leaf-to-callus transition, suggesting that the altered regeneration phenotype of met1-3 was caused by the constitutively hypomethylated genes, independent of the tissue type. In particular, MET1-dependent CG methylation was observed at the blue light receptor genes, CRYPTOCHROME 1 (CRY1) and CRY2, which reduced their expression. Coexpression network analysis revealed that the CRY1 gene was closely linked to cytokinin signaling genes. Consistently, functional enrichment analysis of differentially expressed genes in met1-3 showed that gene ontology terms related to light and hormone signaling were overrepresented. Overall, our findings indicate that MET1-dependent repression of light and cytokinin signaling influences plant regeneration capacity and shoot identity establishment.

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과제정보

This work was supported by the Basic Science Research (NRF-2019R1I1A1A01061376 to S.S.; NRF-2019R1A2C2006915 to P.J.S.) and Basic Research Laboratory (NRF-2020R1A4A2002901 to P.J.S.) programs provided by the National Research Foundation of Korea, by the National Research Foundation of Korea, and by the Creative-Pioneering Researchers Program through Seoul National University (0409-20200281 to P.J.S.).

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