Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Anti-inflammatory Polymethoxyflavones Isolated from the Branches of Shiranuhi Tree

  • Jo, Yeon Jeong (R&D Team, Jeju Industry-University Convergence Center) ;
  • Lee, Nam Ho (Department of Chemistry and Cosmetics, Jeju National University)
  • Received : 2021.06.11
  • Accepted : 2021.07.31
  • Published : 2021.10.20
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Abstract

Shiranuhi is a fruit of Citrus species widely cultivated in Jeju Island, Korea. From an extract of Shiranuhi tree branches were identified five polymethoxyflavones possessing anti-inflammatory effects; nobiletin (1), sinensetin (2), tetramethylscutellarein (3), 6-hydroxy-5,7,3',4'-tetramethoxyflavone (4) and 5-desmethylsinensetin (5). Evaluation of the activities was conducted by monitoring the production of nitric oxide (NO), prostaglandin E2 and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) as well as the levels of iNOS and COX-2 protein expression in LPS-induced RAW264.7 cells. Among the isolates, the compound 4 exhibited the most significant NO inhibition, and suppressed the levels of iNOS and related cytokines. Therefore, it was suggested that the extract and constituents from Shiranuhi tree branches could be useful as anti-inflammatory ingredient.

Keywords

Acknowledgement

This research is supported by the 2020 scientific promotion program funded by Jeju National University.

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