Mycobiology

The Korean Society of Mycology (한국균학회)
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Evaluation of Genetic Diversity and Population Structure Analysis among Germplasm of Agaricus bisporus by SSR Markers

  • An, Hyejin (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Lee, Hwa-Yong (Department of Forest Science, Chungbuk National University) ;
  • Shin, Hyeran (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Bang, Jun Hyoung (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Han, Seahee (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Oh, Youn-Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science) ;
  • Jang, Kab-Yeul (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science) ;
  • Cho, Hyunwoo (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Hyun, Tae Kyung (Department of Industrial Plant Science and Technology, Chungbuk National University) ;
  • Sung, Jwakyung (Department of Crop Science, Chungbuk National University) ;
  • So, Yoon-Sup (Department of Crop Science, Chungbuk National University) ;
  • Jo, Ick-Hyun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science) ;
  • Chung, Jong-Wook (Department of Industrial Plant Science and Technology, Chungbuk National University)
  • Received : 2021.04.12
  • Accepted : 2021.06.07
  • Published : 2021.08.31
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Abstract

Agaricus bisporus is a popular edible mushroom that is cultivated worldwide. Due to its secondary homothallic nature, cultivated A. bisporus strains have low genetic diversity, and breeding novel strains is challenging. The aim of this study was to investigate the genetic diversity and population structure of globally collected A. bisporus strains using simple sequence repeat (SSR) markers. Agaricus bisporus strains were divided based on genetic distance-based groups and model-based subpopulations. The major allele frequency (MAF), number of genotypes (NG), number of alleles (NA), observed heterozygosity (HO), expected heterozygosity (HE), and polymorphic information content (PIC) were calculated, and genetic distance, population structure, genetic differentiation, and Hardy-Weinberg equilibrium (HWE) were assessed. Strains were divided into two groups by distance-based analysis and into three subpopulations by model-based analysis. Strains in subpopulations POP A and POP B were included in Group I, and strains in subpopulation POP C were included in Group II. Genetic differentiation between strains was 99%. Marker AB-gSSR-1057 in Group II and subpopulation POP C was confirmed to be in HWE. These results will enhance A. bisporus breeding programs and support the protection of genetic resources.

Keywords

Acknowledgement

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through the Golden Seed Project, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) [213007-05-5-SBJ30].

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