대한의생명과학회지 (Biomedical Science Letters)

  • 제27권3호
  • /
  • Pages.170-176
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  • 2021
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
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Thapsigargin Induces Platelet Aggregation, thereby Releases Lactate Dehydrogenase from Rat Platelets

  • Baik, Ji Sue (Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Seo, You Na (Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Rhee, Man Hee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Moon-Taek (Research Center, Dongnam Institute of Radiological & Medical Sciences) ;
  • Kim, Sung Dae (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University)
  • 투고 : 2021.09.01
  • 심사 : 2021.09.17
  • 발행 : 2021.09.30
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초록

Thapsigargin (TG), a sarco/endoplasmic reticulum (ER) Ca2+-ATPase (SERCA) inhibitor, has been widely used as an agonist for platelet aggregation for decades. In this study, we investigated the effect of TG on the release of lactate dehydrogenase (LDH) for platelets and elucidated its mechanism. Platelet LDH release and platelet aggregation were increased by TG treatment; 1,000 nM of TG induced the complete lysis of platelets. Other agonists such as collagen (2.5 ㎍/mL), thrombin (0.1 U/mL), and ADP (10 mM) did not induce significant platelet LDH release despite platelet aggregation. Finally, we investigated the effects of pharmacological inhibitors on TG-induced platelet aggregation and LDH release. SP600125, a JNK inhibitor, and LY294002, a PI-3K inhibitor, inhibited TG-induced platelet LDH release but not platelet aggregation. Forskolin, an adenylyl cyclase activator, also inhibited LDH release without affecting platelet aggregation by TG. These results suggest that the TG-induced platelet aggregation was accompanied by LDH release but regulated by a different signaling pathway.

키워드

과제정보

This work was supported by a National Research Foundation of Korea (DIRAMS) grant funded by the Korean government (MSIP) (50591-2021).

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