Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Citric Acid Reduces Alkaline Stress-induced Chlorosis, Oxidative Stress, and Photosynthetic Disturbance by Regulating Growth Performance, Antioxidant Activity and ROS Scavenging in Alfalfa

  • Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Song, Yowook (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Woo, Jae Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Bo Ram (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lim, Eun A (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) ;
  • Rahman, Md Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2021.09.13
  • Accepted : 2021.09.27
  • Published : 2021.09.30
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Abstract

Pollution of agricultural soil by alkaline salts, such as Na2CO3, is a critical and long-lasting problem in cultivable land. The aim of the study was to examine the putative role of citric acid (CA) in alleviating Na2CO3-stress in alfalfa. In this study, Na2CO3 significantly induced leaf chlorosis, inhibited plant growth and photosynthesis related parameters, increased hydrogen peroxide (H2O2) and reduced major antioxidant enzymes (SOD, CAD, APX) in alfalfa. However, the presence of CA these negative effects of Na2CO3-stress largely recovered. Interestingly, expression of antioxidant and ion transporter genes (Fe-SOD, CAT, APX, DHAR and NHX1) involved in Reactive oxygen species (ROS) homeostasis and oxidative stress tolerance in alfalfa. These findings suggest that CA-mediated Na2CO3 stress alleviation is an ecofriendly approach that would be useful to local farmer for alfalfa and other forage crop cultivation in alkaline soils.

Keywords

Acknowledgement

This study was partially funded by the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01592501). This work was also supported by the Postdoctoral Fellowship Program of the National Institute of Animal Science funded by RDA, Republic of Korea.

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