Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Cosmetic Application Using Skin Whitening and Anti-microbial effects of Ethyl Acetate and n-Butanol Fractions from Eruca sativa

Eruca sativa 에칠아세테이트와 부탄올 분획물의 미백 및 항균효과를 이용한 화장품 응용연구

  • Park, Jihye (Department of Chemistry, The Graduate School of Mokwon University) ;
  • Lee, Kwang-ho (Department of Biomedical Systems Engineering, Convergence Technology Campus, Polytechnics University) ;
  • Kim, Bora (Department of Cosmetics & Beauty, Mokwon University)
  • 박지혜 (목원대학교 대학원 화학과) ;
  • 이광호 (한국폴리텍대학 융합기술연구원 생명의료시스템과) ;
  • 김보라 (목원대학교 화장품뷰티학과)
  • Received : 2021.06.02
  • Accepted : 2021.06.25
  • Published : 2021.06.30
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Abstract

Eruca sativa, called arugula, is a perennial plant in the Brassicaceae family, an edible plant commonly used in Italian cuisine. To study as a cosmetic material application E. sativa was extracted with 70% ethanol (ES). Then ES was fractionated using n-hexane, chloroform, ethyl acetate, n-butyl alcohol and water (EHex, EEA, ECHCl3, EBuOH and EDW). EEA showed mushroom tyrosinase inhibitory activity. ES, EEA and EBuOH showed inhibition of tyrosinase activity. As a result, ES is expected to have skin whitening efficacy. ES was applied to 0.05, 0.1% the toner and emulsion formulation to test the stability. The anti-microbial activity of eight bacteria and fungi including Staphylococcus aureus and Propionibacterium acnes which cause dermatitis and acne was evaluated. EEA showed effects in all of microorganisms. The toner and emulsion containing ES with 0.05, 0.1% were passed in the challenge test. At -20, 4, 25, 55 ℃ and daylight, there was no significant change on pH, viscosity for 4 months. However, emulsions had phase separation phenomenon at 55 ℃, so the base formulation needs improvement. In addition, through the skin penetration test, EEA penetrated 0.058% in 6 hr, predicting the clinical efficacy. This means that E. sativa can contribute whitening agent and the synergistic effect of preservatives.

Eruca sativa (E. sativa)는 루꼴라라고 불리우고 이탈리아 요리에 많이 사용되는 식물로 Brassicaceae 속에 속하는 다년생 식물이다. E. sativa를 70% 에탄올로 환류추출 및 농축하고 (ES), n-hexane (EHex), ethyl acetate (EEA), chloroform (ECHCl3), n-butyl alcohol (EBuOH) 및 water (EDW) 용매 극성별로 분획하여 미백 효능을 규명하고 화장품 소재로서의 응용성을 연구하였다. ES, EHex, EEA, ECHCl3, EBuOH 및 EDW의 mushroom tyrosinase 활성 저해를 평가한 결과 EEA가 가장 유의하게 저해하였으며, B16F10 멜라노마 세포 내에서의 멜라닌 생성을 확인한 결과 ES, EEA 및 EBuOH를 처리하였을 때 멜라닌 생성이 유의하게 저해되었으며, 특히 EEA 분획물에서 가장 효과가 뛰어났다. 피부염과 여드름을 일으키는 황색포도상구균, 여드름 원인균을 포함한 8종의 세균, 진균에 대해 ES, EHex, EEA, ECHCl3, EBuOH 및 EDW의 항균력과 방부력 시험을 평가한 결과 EEA는 8종의 균 모두 효과적으로 항균력을 나타냈다. ES를 0.05, 0.1%를 함유하여 제조한 토너와 에멀션은 기존 처방에서 방부력을 증가시켜 천연보존제 역할을 할 수 있을 것으로 판단된다. ES를 토너, 에멀션 제형에 0.05, 0.1%로 첨가하여 일광, -20, 4, 25, 55 ℃ 조건에서 4 개월동안 안정성을 평가한 결과 pH 및 점도에 대한 영향은 크게 없었으나 대조군 및 ES를 첨가한 에멀션 55 ℃에서 분리가 일어나는 것을 확인하였다. 또한, 피부침투시험을 통해 EEA는 6 hr에서 0.058%가 침투되어 임상적인 효능을 예측할 수 있었다. 천연소재 E. sativa는 미백 기능성화장품 소재 및 천연 항균 보존제로 활용 가치가 있을 것으로 사료된다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No. 2020R1A2C1005993).

References

  1. K. N. Yoon, H. S. Jang, G. H. Jin, "Antioxidant, Anti-diabetic, Anti-cholinesterase, and Nitric Oxide Inhibitory Activities of Fruiting Bodies of Agaricus brasiliensis", Korean Journal of Clinical Laboratory Science, Vol.47, No.4, pp. 194-202, (2015). https://doi.org/10.15324/kjcls.2015.47.4.194
  2. H. Y. Park, B. A. Gilchrest, "Signaling pathways mediating melanogenesis", Cellular and Molecular Biology (Noisy-le-Grand, France), Vol.45, No.7, pp. 919-930, (1999).
  3. A. C. De Groot, A. Herxheimer, "IIsothiazolinone preservative: cause of a continuing epidemic of cosmetic dermatitis", The Lancet, Vol.333, No.8633, pp. 314-316, (1989). https://doi.org/10.1016/S0140-6736(89)91318-4
  4. J. Boberg, C. Taxvig, S. Christiansen, U. Hass, "Possible endocrine disrupting effects of parabens and their metabolites", Reproductive Toxicology, Vol.30, No.2, pp. 301-312, (2010). https://doi.org/10.1016/j.reprotox.2010.03.011
  5. J. Y. Lee, J. N. Lee, G. T. Lee, K. K. Lee, "Development of Antimicrobial Plant Extracts and its Application to Cosmetics", Journal of the Society of Cosmetic Scientists of Korea, Vol.38, No.2, pp. 171-129, (2012). https://doi.org/10.15230/SCSK.2012.38.2.171
  6. M. Koubaa, D. Driss, F. Bouaziz, R. E. Ghorbel, S. E. Chaabouni, "Antioxidant and antimicrobial activities of solvent extract obtained from rocket (Eruca sativa L.) flowers", Free Radicals & Antioxidants, Vol.5, No.1, pp. 29-34, (2015). https://doi.org/10.5530/fra.2015.1.5
  7. H. S. Kim, "Anti-melanogenic effect of Eruca sativa extract", Korean Journal of Food Preservation, Vol.23, No.1, pp. 74-79, (2016). https://doi.org/10.11002/KJFP.2016.23.1.74
  8. B. Kim, H. S. Kim, "Antimicrobial activities and skin barrier improvement effect of Eruca sativa extract", Korean Journal of Food Preservation, Vol.24, No.2, pp. 320-324, (2017). https://doi.org/10.11002/KJFP.2017.24.2.320
  9. B. Kim, Y. E. Choi, H. S. Kim, "Eruca sativa and its Flavonoid Components, Quercetin and Isorhamnetin, Improve Skin Barrier Function by Activation of Peroxisome Proliferator-Activated Receptor (PPAR)-α and Suppression of Inflammatory Cytokines", Phytotherapy Research, Vol.28, No.9, pp. 1359-1366, (2014). https://doi.org/10.1002/ptr.5138
  10. L. Z. Piao, H. R. Park, Y. K. Park, S. K. Lee, J. H. Park, M. K. Park, "Mushroom Tyrosinase Inhibition Activity of Some Chromones", Chemical and Pharmaceutical Bulletin, Vol.50, No.3, pp. 309-311, (2002). https://doi.org/10.1248/cpb.50.309
  11. I. Chiocchio, M. Mandrone, P. Tomasi, L. Marincich, F. Poli, "Plant Secondary Metabolites: An Opportunity for Circular Economy", Molecules, Vol.26, No.2. pp. 495-525, (2021). https://doi.org/10.3390/molecules26020495
  12. D. F. Hernandez, E. L. Cervantes, D. A. Luna-Vital, L. Mojica, "Food-derived bioactive compounds with anti-aging potential for nutricosmetic and cosmeceutical products", Critical Reviews in Food Science and Nutrition, Vol.10, No.1, pp. 16, (2020).
  13. M. Taviano, A. Melchini, A. Filocamo, C. Costa, S. Catania, R. Raciti, S. Saha, P. Needs, G. G. Bisignano, N. Miceli, "Contribution of the Glucosinolate Fraction to the Overall Antioxidant Potential, Cytoprotection against Oxidative Insult and Antimicrobial Activity of Eruca sativa Mill. Leaves Extract", Pharmacognosy Magazine, Vol.13, No.52, pp. 738-743, (2017). https://doi.org/10.4103/pm.pm_245_16
  14. M. Gulfraz, A. Sadiq, H. Tariq, M. Imran, R. Qureshi, A. Zeenat, "Phytochemical analysis and antibacterial activity of Eruca sativa seed", Pakistan Journal of Botany, Vol.43, No.2, pp. 1351-1359, (2011).
  15. A. Nowak, K. Cybulska, E. Makuch, L. Kucharski, M. Rozewicka-Czabanska, P. Prowans, N. Czapla, P. Bargiel, J. Petriczko, A. Klimowicz, "In Vitro Human Skin Penetration, Antioxidant and Antimicrobial Activity of Ethanol-Water Extract of Fireweed (Epilobium angustifolium L.)", Vol.26, No.2. pp. 329-347, (2021) https://doi.org/10.3390/molecules26020329