한국해양생명과학회지 (Journal of Marine Life Science)

한국해양생명과학회 (The Korean Society of Marine Life Science)
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미역과 쇠미역 추출물의 항산화 및 간 보호 효과

Antioxidative Activities and Protective Effects on Alcohol-Induced Oxidative Stress in the Human Hepatic HepG2 Cells of Undaria pinnatifida and Costaria costata Extracts

  • 김기안 ((재)전남바이오산업진흥원 해양바이오연구센터) ;
  • 오태환 ((재)전남바이오산업진흥원 해양바이오연구센터) ;
  • 천상호 ((재)전남바이오산업진흥원 해양바이오연구센터)
  • Kim, Ki An (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation) ;
  • Oh, Tae-Hwan (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation) ;
  • Chun, Sang-Ho (Marine Biotechnology Research Center, Jeonnam Bioindustry Foundation)
  • 투고 : 2021.10.15
  • 심사 : 2021.11.15
  • 발행 : 2021.12.15
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초록

본 연구는 미역(Undaria pinnatifida) 추출물과 쇠미역(Costaria costata) 추출물의 항산화 활성 및 HepG2 세포의 알코올로 인한 산화 손상에 대한 보호 효과를 연구하였다. 미역과 쇠미역 추출물의 총 폴리페놀과 플라보노이드 함량은 70% 에탄올 추출물에서 가장 높았다. 또한 미역과 쇠미역 70% 에탄올 추출물의 DPPH (IC50 0.33±0.21, 0.48±0.47 mg/ml), ABTS (IC50 0.34±0.30, 0.47±0.17 mg/ml) 라디칼 소거 활성이 열수 추출물 및 10% 에탄올 추출물 보다 높았다. 추출물의 간 보호 효과를 확인하기 위하여 HepG2 세포에 알코올 산화 스트레스를 유발하여 MTT 분석을 이용하여 세포 생존력을 측정하였다. 미역 및 쇠미역 열수 추출물은 알코올 처리군(73.95%) 대비 세포 생존율은 각각 89.91~97.63% 및 84.99~90.54%로 농도 의존적으로 증가시켰다. 본 연구는 미역과 쇠미역 추출물이 간 보호 및 항산화 효과를 나타냄을 확인하였고 알코올 산화 손상에 대한 간 보호 소재로 활용될 수 있을 것으로 사료된다.

We investigated the antioxidant and hepatoprotective effects of extracts from the Undaria pinnatifida and Costaria costata against ethanol-induced oxidative damage. The total polyphenol and flavonoid contents were highest in the 70% ethanol extract from Undaria pinnatifida and Costaria costata. Also, the radical scavenging activity of DPPH (IC50 0.33± 0.21, 0.48±0.47 mg/ml) and ABTS (IC50 0.34±0.30, 0.47±0.17 mg/ml) in the 70% ethanol extract was higher than that of the hot water and 10% ethanol extracts. To determine the hepatoprotective effects of extracts in ethanol-induced oxidative damage, cell viability was measured using an MTT assay. In the pre-treatment of Undaria pinnatifida and Costaria costata hot water extracts, the concentration-dependent increased the cell viability compared with the ethanol treated cells (73.95%) by 89.91~97.63% and 84.99~90.54%, respectively. The data suggests that 70% ethanol extracts have antioxidant activity and hot water extracts exhibit hepatoprotective effects. Therefore, Undaria pinnatifida and Costaria costata may be considered potential agents for control ethanol-induced liver damage.

키워드

과제정보

본 연구는 전라남도와 (재)전남테크노파크가 지원한 '2020년도 하반기 지역수요맞춤형 연구개발사업' 지원을 받아 수행된 연구결과로 이에 감사드립니다.

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