대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제47권2호
  • /
  • Pages.133-138
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  • 2021
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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나이아신아마이드의 인공피부조직 투과 개선을 위한 고형지질나노입자의 설계 연구

Design of Solid Lipid Nanoparticles to Improve Penetration of Niacinamide through Artificial Skin Tissue

  • Yeo, Sooho (College of Pharmacy, Chung-Ang University)
  • 투고 : 2021.05.11
  • 심사 : 2021.06.09
  • 발행 : 2021.06.30
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초록

본 연구에서는 기능성 미백물질인 나이아신아마이드(NI)의 피부 투과율을 개선시키기 위해 NI를 고형지질 나노입자(solid lipid nanoparticles, SLNs))에 봉입하고 피부 투과율을 평가하였다. NI는 이중 에멀션 가온용융 유화법으로 SLNs 내에 효과적으로 봉입할 수 있었으며, 평균 입자 크기는 263.30 ~ 436.93 nm이었고 제타전위는 -34.77 ~ -57.60 mV인 안정한 입자를 제조하였다. NI의 피부 투과 연구를 위해 인체 표피 조직에서 유래된 피부각질세포로 만든 3차원적 인공피부(SkinEthicTM RHE)을 사용하였다. NI의 피부 투과 및 침적 실험 결과 모든 SLNs 제형이 NI의 피부 투과 및 침적율을 개선시켰으며, SLNs을 적용하였을 때가 그렇지 않을 때 보다 투과율은 약 5.4 ~ 7.6 배, 침적율은 9.5 ~ 20.8 배 개선되었을을 확인하였다. 따라서, 본 연구에서 제조된 고형지질나노입자는 기능성 미백물질인 나이아신아마이드의 피부 투과율을 개선하기에 충분한 결과를 보여 주었다.

In this study, Niacinamide (NI) was loaded into solid lipid nanoparticles (SLNs) and skin permeability was evaluated to improve skin permeability of NI, which was a skin whitening substance. NI was able to effectively load within SLN with a double-melting emulsification method, producing stable particles with average particle sizes of 263.30 to 436.93 nm and a zeta potential of -34.77 to -57.60 mV. Artificial skin tissue (SkinEthicTM RHE) derived from skin keratinocytes derived from human epidermal tissue was used for the skin permeation study of NI. Skin transmittance and deposition experiments of NI confirmed that all SLN formulations improved skin transmittance and deposition rates of NI, approximately 5.4 ~ 7.6 and 9.5 ~ 20.8 improvement over SLN applications. Therefore, SLN manufactured in this study have shown sufficient results to improve the skin permeability of the functional whitening substance, NI.

키워드

참고문헌

  1. J. W. Li n, H. M. Chi ang, Y. C. Li n, and K. C. Wen, Natural products with skin-whitening effects, J. Food Drug Anal., 16(2), 1 (2008).
  2. T. Hakozaki, L. Minwalla, J. Zhuang, M. Chhoa, A. Matsubara, K. Miyamoto, A. Greatens, G. G. Hillebrand, D. L. Bissett, and R. E. Boissy, The effect of niacinamide on reducing cutaneous pigmentation and suppression of melanosome transfer, Br. J. Dermatol., 147(1), 20 (2002). https://doi.org/10.1046/j.1365-2133.2002.04834.x
  3. M. H. Lee, K. K. Lee, M. H. Park, S. S. Hyun, S. Y. Kahn, K. S. Joo, H. C. Kang, and W. T. Kwon, In vivo anti-melanogenesis activity and in vitro skin permeability of niacinamide-loaded flexible liposomes (BounsphereTM), J. Drug Deliv. Sci. Technol., 31, 147 (2016). https://doi.org/10.1016/j.jddst.2015.12.008
  4. J. S. Lucks, R. H. Muller, and B. Konig, Solid lipid nanoparticles (SLN)-an alternative parenteral drug carrier system, Eur. J. Pharm. Biopharm., 38(33), 149 (1992).
  5. U. Gonullu, M. Uner, G, Yener, E. F. Karaman, and Z. Aydogmus, Formulation and characterization of solid lipid nanoparticles, nanostructured lipid carriers and nanoemulsion of lornoxicam for transdermal delivery, Acta Pharm., 65(1), 1(2015). https://doi.org/10.1515/acph-2015-0009
  6. K. Mitri, R. Shegokar, S. Gohla, C. Anselmi, and R. H. Muller, Lipid nanocarriers for dermal delivery of lutein: preparation, characterization, stability and performance, Int. J. Pharm., 414(1-2), 267 (2011). https://doi.org/10.1016/j.ijpharm.2011.05.008
  7. C. Lotte, C. Patouillet, M. Zanini, A. Messager, and R. Roguet, Permeation and skin absorption: reproducibility of various industrial reconstructed human skin models, Skin Pharmacol. Physiol., 15(Suppl. 1), 18 (2002). https://doi.org/10.1159/000066679
  8. D. Monti, P. Chetoni, S. Burgalassi, S. Tampucci, M. Centini, and C. Anselmi, 4-Methylbenzylidene camphor microspheres: reconstituted epidermis (Skinethic®) permeation and distribution, Int. J. Cosmet. Sci., 37(3), 298 (2015). https://doi.org/10.1111/ics.12199