대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제47권2호
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  • Pages.123-131
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  • 2021
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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흑효모를 이용한 참도박 발효 추출물의 항염 효과

Anti-inflammatory effects of Grateloupia elliptica Fermenting Extracts Using Aureobasidium pullulans

  • 부반빈 (영남대학교 생명응용과학대학 생명공학과) ;
  • 이경은 (영남대학교 생명응용과학대학 생명공학과) ;
  • 강상구 (영남대학교 생명응용과학대학 생명공학과)
  • Vu, Van Vinh (Department of Biotechnology, College of Life and applied Sciences, Yeungnam University) ;
  • Lee, Kyung Eun (Department of Biotechnology, College of Life and applied Sciences, Yeungnam University) ;
  • Kang, Sang Gu (Department of Biotechnology, College of Life and applied Sciences, Yeungnam University)
  • 투고 : 2021.02.26
  • 심사 : 2021.04.27
  • 발행 : 2021.06.30
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⟨ 이전 논문 다음 논문 ⟩

초록

해양 홍조류 참도박(Grateloupia elliptica, G. elliptica)을 흑효모(Aureobasidium pullulans, A. pullulans)로 발효시킨 추출물들의 인간 피부각질세포주(HaCaT)에 대한 항염증 효과를 조사하였다. 흑효모로 발효한 참도박 추출물은 참도박 추출물에 비하여 총 폴리페놀(total polyphenol content)은 최대 2.7 배로 증가되었으며 총 플라보노이드(total flavonoid conten)는 최대 2.4 배 증가되었다. 인간 피부각질세포를 lipopolysaccharide (LPS) 또는 H2O2로 염증을 유도 한 후 참도박 추출물과 참도박 발효 추출물을 100 ㎍/mL 농도로 처리 한 결과 LPS 또는 H2O2 만 처리한 세포군에 비하여 약 3 ~ 10% 정도 세포생존율이 증가되었다. 또한 염증 유발과 관련된 단백질 발현 변화를 조사한 결과 참도박 발효추출물이cyclooxygenase-2 및 70 kDa heat shock protein 의 발현을 현저히 감소시켰다. 따라서 흑효모로 발효한 참도박은 항염효과를 가진 피부수렴용 화장품 소재로 유용하다.

In this study, we investigated the biological functions of Grateloupia elliptica (G. elliptica) fermented with Aureobasidium pullulans (A. pullulans). Total phenolic contents (TPC) of the hot-water extract of the fermented G. elliptica increased 2.7 folds than that of the non-fermented G. elliptica. Furthermore, total flavonoid contents of both the hot-water extract and the ethanol extract increased maximum 2.4 folds amounts than non-fermented G. elliptica extracts.HaCaT cells were induced inflammation treated with LPS (1 ㎍/mL) or H2O2 (1mM) and examined with 100 ㎍/mL of G. elliptica extracts. The extraction of the fermented G. elliptica increased HaCaT cell proliferation in the maximum 10% than non-fermented G. elliptica extraction. Furthermore, investigating changes in protein expression associated with inflammation resulted in a significant reduction in the expression of cyclooxygenase-2 and 70 kDa heat shock proetin. Conclusively, the extracts of G. elliptica fermented with A. pullulans have bioactive functions both anti-oxidant to protect environmental stresses and anti-inflammation activity. Hence, G. elliptica fermented with A. pullulans would be a good natural resource as bioactive ingredients for cosmetics. Therefore, G. elliptica fermented with A. pullulans is useful as a astringent material with anti-inflammatory skin.

키워드

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