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New records of five taxa of unarmored and thin-walled dinoflagellates from brackish and coastal waters of Korea

  • Hojoon Choi (Division of Earth Environmental System Science, Pukyong National University) ;
  • Minji Cho (Division of Earth Environmental System Science, Pukyong National University) ;
  • Sunju Kim (Division of Earth Environmental System Science, Pukyong National University)
  • Received : 2021.12.14
  • Accepted : 2021.12.20
  • Published : 2021.12.31

Abstract

Unarmored and thin-walled dinoflagellates were collected from brackish and coastal waters of Korea from August 2019 to August 2021. A total of 10 species belonging to orders Sussiales and Gymnodiniales were isolated and established as clonal cultures. Of them, five species (Biecheleria brevisulcata, Lepidodinium chlorophorum, Karlodinium decipiens, Kirithra asteri, and Wangodinium sinense) are newly recorded in Korea and examined using a light microscope (LM) and a scanning electron microscope (SEM). Their molecular phylogeny was inferred from LSU rDNA sequences. Here, we present taxonomic information, morphological features, and molecular phylogenetic positions of these unrecorded dinoflagellate species.

Keywords

Acknowledgement

This work was supported by a Research Grant of Pukyong National University(2021).

References

  1. Anderson DM, AD Cembella and GM Hallegraeff. 2012. Progress in understanding harmful algal blooms: paradigm shifts and new technologies for research, monitoring, and management. Annu. Rev. Mar. Sci. 4:143-176. https://doi.org/10.1146/annurev-marine-120308-081121
  2. Archibald JM. 2009. The puzzle of plastid evolution. Curr. Biol. 19:R81-R88. https://doi.org/10.1016/j.cub.2008.11.067
  3. Benico G, K Takahashi, WM Lum, AT Yniguez and M Iwataki. 2020. The harmful unarmored dinoflagellate Karlodinium in Japan and Philippines, with reference to ultrastructure and micropredation of Karlodinium azanzae sp. nov.(Kareniaceae, Dinophyceae). J. Phycol. 56:1264-1282. https://doi.org/10.1111/jpy.13030
  4. Boutrup PV, O Moestrup, U Tillmann and N Daugbjerg. 2017. Ultrastructure and phylogeny of Kirithra asteri gen. et sp. nov. (Ceratoperidiniaceae, Dinophyceae) - a free-living, thin-walled marine photosynthetic dinoflagellate from Argentina. Protist 168:586-611. https://doi.org/10.1016/j.protis.2017.08.001
  5. Choi H and S Kim. 2021. Heterocapsa busanensis sp. nov. (Peridiniales, Dinophyceae): A new marine thecate dinoflagellate from Korean coastal waters. Eur. J. Protistol. 79:125797.
  6. Daugbjerg N, G Hansen, J Larsen and O Moestrup. 2000. Phylogeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data, including the erection of three new genera of unarmoured dinoflagellates. Phycologia 39:302-317. https://doi.org/10.2216/i0031-8884-39-4-302.1
  7. de Salas M, A Laza-Martinez and GM Hallegraeff. 2008. Novel unarmored dinoflagellates from the toxigenic family Kareniaceae (Gymnodiniales): five new species of Karlodinium and one new Takayama from the Australian sector of the Southern Ocean. J. Phycol. 44:241-257. https://doi.org/10.1111/j.1529-8817.2007.00458.x
  8. Elbrachter M and E Schnepf. 1996. Gymnodinium chlorophorum, a new, green, bloom-forming dinoflagellate (Gymnodiniales, Dinophyceae) with a vestigial prasinophyte endosymbiont. Phycologia 35:381-393. https://doi.org/10.2216/i0031-8884-35-5-381.1
  9. Fensome RA, FJR Taylor, G Norris, WAS Sarjeant, DI Wharton and GL Williams. 1993. A classification of living and fossil dinoflagellates (Special publication number 7). Micropaleontology 7:1-351.
  10. Flo Jorgensen M, S Murray and N Daugbjerg. 2004. A new genus of athecate interstitial dinoflagellates, Togula gen. nov., previously encompassed within Amphidinium sensu lato: Inferred from light and electron microscopy and phylogenetic analyses of partial large subunit ribosomal DNA sequences. Phycol. Res. 52:284-299. https://doi.org/10.1111/j.1440-1835.2004.tb00338.x
  11. Garate-Lizarraga I, MS Muneton-Gomez, B Perez-Cruz and JA Diaz-Ortiz. 2014. Bloom of Gonyaulax spinifera(Dinophyceae: Gonyaulacales) in Ensenada de La Paz Lagoon, Gulf of California. CICIMAR Oceanides 29:11-18.
  12. Gomez F. 2012. A checklist and classification of living dinoflagellates (Dinoflagellata, Alveolata). CICIMAR Oceanides 27:65-140. https://doi.org/10.37543/oceanides.v27i1.111
  13. Guillard RR and JH Ryther. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran. Can. J. Microbiol. 8:229-239. https://doi.org/10.1139/m62-029
  14. Hehenberger E, RB Gast and PJ Keeling. 2019. A kleptoplastidic dinoflagellate and the tipping point between transient and fully integrated plastid endosymbiosis. Mol. Biol. Evol. 17:718-729.
  15. Kim S and MG Park. 2014. Amoebophrya spp. from the bloom forming dinoflagellate Cochlodinium polykrikoides: parasites not nested in the "Amoebophrya ceratii complex". J. Eukaryot. Microbiol. 61: 173-181. https://doi.org/10.1111/jeu.12097
  16. Katoh K and DM Standley. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol. Biol. Evol. 30:772-780. https://doi.org/10.1093/molbev/mst010
  17. Katoh K, J Rozewicki and KD Yamada. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinform. 20:1160-1166. https://doi.org/10.1093/bib/bbx108
  18. Lindberg K, O Moestrup and N Daugbjerg. 2005. Studies on woloszynskioid dinoflagellates I: Woloszynskia coronata re-examined using light and electron microscopy and partial LSU rDNA sequences, with description of Tovellia gen. nov. and Jadwigia gen. nov. (Tovelliaceae fam. nov.). Phycologia 44:416-440. https://doi.org/10.2216/0031-8884(2005)44[416:SOWDIW]2.0.CO;2
  19. Luo Z, Z Hu, YZ Tang, KN Mertens, CP Leaw, PT Lim, ST Teng, L Wang and H Gu. 2018. Morphology, ultrastructure and molecular phylogeny of Wangodinium sinense gen. et sp. nov. (Gymnodiniales, Dinophyceae) and revisiting of Gymnodinium dorsalisulcum and Gymnodinium impudicum. J. Phycol. 54:744-761. https://doi.org/10.1111/jpy.12780
  20. McCarthy PM. 2013. Census of Australian Marine Dinoflagellates. Australian Biological Resources Study, Canberra. https://www.anbg.gov.au/abrs/Dinoflagellates/index_Dino.html [version 11 July 2013]. Australian Biological Resources Study. Canberra.
  21. Moestrup O and N Daugbjerg. 2007. On dinoflagellate phylogeny and classification. pp. 215-230. In: Unravelling the Algae: The Past, Present, and Future of Algae Systematics (Brodie J and J Lewis, eds.). Systematics Association Special Volumes. CRC Press. Boca Raton, FL.
  22. Moestrup O, K Lindberg and N Daugbjerg. 2009. Studies on woloszynskioid dinoflagellates IV: the genus Biecheleria gen. nov. Phycol. Res. 57:203-220. https://doi.org/10.1111/j.1440-1835.2009.00540.x
  23. Nunn GB, BF Theisen, B Christensen and P Arctander. 1996. Simplicity-correlated size growth of the nuclear 28S ribosomal RNA D3 expansion segment in the crustacean order Isopoda. J. Mol. Evol. 42:211-223. https://doi.org/10.1007/BF02198847
  24. Rhodes LL, KF Smith, L MacKenzie and C Moisan. 2019. Checklist of the planktonic marine dinoflagellates of New Zealand. N. Z. J. Mar. Freshw. Res. 53:1-16. https://doi.org/10.1080/00288330.2019.1531462
  25. Rene A, J Camp and E Garces. 2015. Diversity and phylogeny of Gymnodiniales (Dinophyceae) from the NW Mediterranean Sea revealed by a morphological and molecular approach. Protist 166:234-263. https://doi.org/10.1016/j.protis.2015.03.001
  26. Scholz B and G Liebezeit. 2012. Microphytobenthic dynamics in a Wadden sea intertidal flat - Part II: Seasonal and spatial variability of non-diatom community components in relation to abiotic parameters. Eur. J. Phycol. 47:120-137. https://doi.org/10.1080/09670262.2012.665251
  27. Smayda TJ. 1997. Harmful algal blooms: their ecophysiology and general relevance to phytoplankton blooms in the sea. Limnol. Oceanogr. 42:1137-1153. https://doi.org/10.4319/lo.1997.42.5_part_2.1137
  28. Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  29. Takahashi K, C Sarai and M Iwataki. 2014. Morphology of two marine woloszynskioid dinoflagellates, Biecheleria brevisulcata sp. nov. and Biecheleriopsis adriatica (Suessiaceae, Dinophyceae), from Japanese coasts. Phycologia 53:52-65. https://doi.org/10.2216/13-192.1
  30. Tengs T, OJ Dahlberg, K Shalchian-Tabrizi, D Klaveness, K Rudi, CF Delwiche and KS Jakobsen. 2000. Phylogenetic analyses indicate that the 19'Hexanoyloxy-fucoxanthincontaining dinoflagellates have tertiary plastids of haptophyte origin. Mol. Biol. Evol. 17:718-729. https://doi.org/10.1093/oxfordjournals.molbev.a026350
  31. Veen A, CHJ Hof, FAC Kouwets and T Berkhout. 2015. Rijkswaterstaat Waterdienst, Informatiehuis Water [Taxa Watermanagement the Netherlands (TWN)] https://ipt.nlbif.nl/ipt/resource?r=checklist-twn. Consulted March 2017. pp. on line. In the Netherlands, Laboratory for Hydrobiological Analysis, Rijkswaterstaat.