Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Distribution of Tetracycline-Resistance Genes detected from isolates of cultured fishes in Gyeonggi-do

경기도 내 양식어류에서 분리한 병원성 세균의 Tetracycline 내성 유전자 분포

  • Cho, Ki-Taek (Gyeonggi Province Maritime&Fisheries Research Institute) ;
  • Hwang, Yun-Jeong (Gyeonggi Province Maritime&Fisheries Research Institute) ;
  • Lee, Sang-Woo (Gyeonggi Province Maritime&Fisheries Research Institute) ;
  • Kim, Kwang-Il (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
  • 조기택 (경기도 해양수산자원연구소) ;
  • 황윤정 (경기도 해양수산자원연구소) ;
  • 이상우 (경기도 해양수산자원연구소) ;
  • 김광일 (부경대학교 수산생명의학과) ;
  • 정현도 (부경대학교 수산생명의학과)
  • Received : 2021.05.27
  • Accepted : 2021.06.07
  • Published : 2021.06.30
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Abstract

Tetracycline (TC) is one of the antibiotics used for treatment of bacterial infection in Korea. Inadequate usage and abuse cause the resistance to antibiotics, like Tetracycline, Erythromycin, and Fluoroquinolone. It can also affect severe economic loss in aquaculture field in Korea. We isolated 101 bacterial samples from diseased fish at aquaculture sites in Gyeonggi-do during 2015~2018. Minimum inhibitory concentration (MIC) method has been used to determine distribution and to identify bacterial isolates resistant to antibiotics including Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN). TC resistant isolates were confirmed antibiotic resistant genes by conventional PCR. Bacterial isolates were identified as Aeromonas spp. (43.5%), Pseudomonas spp. (4.0%) and Vibrio spp. (5.0%). It was confirmed that multi-resistant isolates (77.2%) were predominant over single-resistant one (22.8%). TC resistant genes like tet(A), tet(D), tet(E), tet(G), tet(M), and tet(S) were detected and tet(A) was the most prevalent. Aeromonas spp. is a dominant strain in bacterial infections in fishes of aquaculture sites, and further investigation on various antibiotic resistance genes will be needed for clear understanding of aquaculture sites in Gyeonggi-do.

Tetracycline (TC)은 국내에서 세균 감염 치료에 반드시 필요한 대표적인 항생제로 알려져 있다. 그러나 부적절한 사용과 남용으로 인해 Tetracycline, Erythromycin, Fluoroquinolone 등과 같은 항생제에 대한 내성이 발생하고 있으며, 이는 국내 양식 분야에서 심각한 경제적 피해를 유발한다. 본 연구에서는 2015~2018년에 걸쳐 경기도 양식장의 양식생물에서 101개 균주를 분리하였다. 분리균주는 간이적인 생화학적 방법을 통해 동정하였으며, 최소 억제농도(MIC)의 확인을 통해 Oxytetracycline (OTC), Ampicillin (AMP), Clindamycin (CLI), Enrofloxacin (ENRO), Gentamycin (GEN)에 대한 내성 여부를 확인하였다. 이중 TC에 내성을 보이는 균주는 PCR법을 통해 tet 유전자의 분포를 조사하였다. 그 결과, 총 101개 균주 중에서 Aeromonas spp.가 44개(43.5%)로 가장 우점하였고, 그 다음으로 Pseudomonas spp. 4개(4.0%), Vibrio spp. 5개(5.0%)가 확인되었다. 또한, 다중 내성을 보이는 균주(77.2%)가 단일 내성균(22.8%)보다 많음을 확인하였다. tet(A), tet(D), tet(E), tet(G), tet(M), tet(S)가 TC 내성 균주에서 검출되었으며 tet(A)가 가장 우점적으로 확인되었다. Aeromonas spp.는 분리된 균주 중에서 가장 많았으며, 경기도 내 양식현장에서의 다양한 항생제 내성 유전자의 특성에 대한 추가적인 연구가 필요하다.

Keywords

Acknowledgement

이 논문은 경기도해양수산자원연구소의 본예산사업 중 수산질병관리원 운영 및 시험연구사업 예산에 의해 수행되었습니다.

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