Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Efficacy of live NNV immersion vaccination in three grouper species (Seven band grouper Hyporthodus septemfasciatus, Red spotted grouper Epinephelus akaara, Long tooth grouper Epinephelus bruneus)

바리과 어류 3종(능성어 Hyporthodus septemfasciatus, 붉바리 Epinephelus akaara, 자바리 Epinephelus bruneus)에 대한 신경괴사증바이러스(Nervous Necrosis Virus, NNV)의 생백신 처리 효과

  • Kim, Si-Woo (Department of Aqualife Medicine, Chonnam National University) ;
  • Park, Jung-Jun (Aquaculture Management Division, National Institute of Fisheries Science) ;
  • Hong, Chang-Gi (A South Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • 김시우 (전남대학교 수산생명의학과) ;
  • 박정준 (국립수산과학원 전략양식부 양식관리과) ;
  • 홍창기 (국립수산과학원 남해수산연구소) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Received : 2021.02.17
  • Accepted : 2021.03.24
  • Published : 2021.06.30
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Abstract

In this study, we investigated safety and efficacy of a low temperature immunization protocol with NNV in red spotted grouper, Epinephelus akaara and long tooth grouper, Epinephelus bruneus and seven band grouper, Hyporthodus septemfasciatus. Further, growth rate between immunized and naïve fish was evaluated during the experiment to check side effect of immunization. Three grouper species were immunized by immersion method with live NNV at 105.0 TCID50/mL at 16.5℃ for 30 min and reared for 120 days at natural sea water temperature. To evaluate growth rate, total length and wet weight was measured 7 times after immunization. Immunized three grouper species were challenged by intramuscular inoculation with NNV at 104.2 TCID50/100 µL/fish. Immunization at low temperature with live NNV did not show any clinical symptoms of infection, mortality and inhibition of growth. After challenge, cumulative mortality of naïve seven band grouper, red spotted grouper, long tooth grouper were 45, 10, 20 %, respectively. However no mortality was observed at immunized groupers. Thus, it was demonstrated that immunization at low temperature with live NNV are able to protect three different species of groupers without inhibition of growth.

Keywords

Acknowledgement

본 논문은 2021년도 국립수산과학원 수산시험연구사업 "주요 양식품종 모니터링(R2021003)의 지원으로 수행된 연구입니다.

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