한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Assessment of the ozonation against pathogenic bacteria in the effluent of the quarantine station

  • Park, Seon Yeong (Program in Environmental and Polymer Engineering, INHA University) ;
  • Kim, Joo Han (Department of Environmental Engineering, INHA University) ;
  • Kim, Chang Gyun (Program in Environmental and Polymer Engineering, INHA University)
  • 투고 : 2021.03.15
  • 심사 : 2021.06.08
  • 발행 : 2021.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study investigated how ozone treatment can successfully inactivate pathogenic bacteria in both artificial seawater and effluents discharged from the fishery quarantine station in Pyeongtaek Port, Korea. Vibrio sp. and Streptococcus sp. were initially inoculated into the artificial seawater. All microbes were almost completely inactivated within 10 min and 30 min by injecting 6.4 mg/min and 2.0 mg/min of ozone, respectively. It was discovered that the water storing Pleuronichthys, Pelteobagrus, and Cyprinus imported from China contained the indicator bacteria, Vibrio sp., Enterococcus sp., total coliforms, and heterotrophic microorganisms. Compared to the control, three indicator bacteria were detected at two to six times higher concentrations. The water samples displayed a diverse microbial community, comprising the following four phyla: Bacteroidetes, Proteobacteria, Firmicutes, and Actinobacteria. Almost all indicator bacteria were inactivated in 5 min at 2.0 mg/min of ozonation; comparatively, 92.9%-98.2% of the less heterotrophic microorganisms were deactivated within the same time period. By increasing the dosage to 6.4 mg/min, 100% deactivation was achieved after 10 min. Despite the almost complete inactivation of most indicator bacteria at high doses after 10 min, several bacterial strains belonging to the Proteobacteria have still been found to be resistant under the given operational conditions.

키워드

과제정보

This research was part of a project entitled "Development of a water treatment system to remove harmful substances of ecological disturbances emitted from quarantine stations screening up imported fishery products" (No. 20180341) supported by the Korea Institute of Marine Science and Technology Promotion. It was also partially supported by INHA University Grant. In addition, the employed marine bacteria for the disinfection test were provided by the Korean Culture collection of Aquatic Microorganisms (KoCAM) under the National Institute of Fisheries Science.

참고문헌

  1. Food and Agriculture Organization of the United Nations (FAO). 2016. The state of world fisheries and aquaculture 2016: Contributing to food security and nutritions for all. Rome, 1-200.
  2. Ministry of Agriculture, Food and Rural Affairs, Korea Agro-Fisheries & Food Trade Corporation. 2018. Trends reports on the Imports and Exports of Agro-fisheries and Food 2017. Korea, 1-518.
  3. Litchman, E., 2010. Invisible invaders: non-pathogenic invasive microbes in aquatic and terrestrial ecosystems. Ecol. Lett. 13, 1560-1572. https://doi.org/10.1111/j.1461-0248.2010.01544.x
  4. Gollasch, S., Minchin, D., David, M. 2015. The transfer of harmful aquatic organisms and pathogens with ballast water and their impact. In: David M., Gollasch, S. (eds) Global Maritime Transport and Ballast Water Management. Invading Nature-Springer Series in Invasion Ecology, Springer. 8, 35-58.
  5. Seebens, H., Gastner, M.T., Blasius, B. 2013. The risk of marine bioinvasion caused by global shipping. Ecol. Lett. 16(6), 782-790. https://doi.org/10.1111/ele.12111
  6. Dobbs, F.C., Rogerson, A. 2005. Ridding ships' ballast water of microorganisms. Environ. Sci. Technol. 39(12), 259A-264A. https://doi.org/10.1021/es053300v
  7. International Maritime Organization (IMO). 2004. International Convention for the Control and Management of Ship's Ballast Water and Sediments.
  8. Drillet, G. 2016. Food security: Protect aquaculture from ship pathogens. Nature. 539(7627), 31. https://doi.org/10.1038/539031d
  9. Lymperopoulou, D.S., and Dobbs, F.C. 2017. Bacterial diversity in ships' ballast water, ballast-water exchange, and implications for ship-mediated dispersal of microorganisms. Environ. Sci. Technol. 51(4), 1692-1972.
  10. Khandeparker, L., Kuchi, N., Desai, D.V., and Anil, A.C. 2020. Changes in the ballast water tank bacterial community during a trans-sea voyage: Elucidation through next generation DNA sequencing. J. Environ. Manage. 273, 111018. https://doi.org/10.1016/j.jenvman.2020.111018
  11. Jung, Y., Yoon, Y., Hong, E., Kwon, M., and Kang, J. W. 2013. Inactivation characteristics of ozone and electrolysis process for ballast water treatment using B. subtilis spores as a probe. Mar. Pollut. Bull. 72(1), 71-79. https://doi.org/10.1016/j.marpolbul.2013.04.028
  12. Bai, M., Zheng, Q., Tian, Y., Zhang, Z., Chen, C., Cheng, C., and Meng, X. 2016. Inactivation of invasive marine species in the process of conveying ballast water using OH based on a strong ionization discharge. Water Res. 96, 217-224. https://doi.org/10.1016/j.watres.2016.03.056
  13. Romero-Martinez, L., Rivas-Zaballos, I., Moreno-Andres, J., Moreno-Garrido, I., Acevedo-Merino, A., and Nebot. E. 2020. Effect of length of dark storage following ultraviolet irradiation of Tetraselmis suecica and its implications for ballast water management, Sci. Total Environ. 711, 134611. https://doi.org/10.1016/j.scitotenv.2019.134611
  14. Maranda, L., Cox, A. M., Campbell, R. G., and Smith, D. C. 2013. Chlorine dioxide as a treatment for ballast water to control invasive species: Shipboard testing. Mar. Pollut. Bull. 75(1-2), 76-89. https://doi.org/10.1016/j.marpolbul.2013.08.002
  15. Goncalves, A. A., and Gagnon, G. A. 2011. Ozone application in recirculating aquaculture system: An overview. Ozone. Sci. Eng. 33(5), 345-367. https://doi.org/10.1080/01919512.2011.604595
  16. Shirafkan, A., Nowee, S. M., Ramezanian, N., and Etermadi, M. M. 2016. Hybrid coagulation/ozonation treatment of pharmaceutical wastewater using ferric chloride, polyaluminum chloride and ozone. Int. J. Environ. Sci. Technol. 13(6), 1443-1452. https://doi.org/10.1007/s13762-016-0965-8
  17. Mainardis, M., Buttazzoni, M., De Bortoli, N., Mion, M. and Goi, D. 2020. Evaluation of ozonation applicability to pulp and paper streams for a sustainable wastewater treatment. J. Clean. Prod. 258, 120781. https://doi.org/10.1016/j.jclepro.2020.120781
  18. Rahmadi, P., Kim, Y. R. 2013. Effects of different levels of ozone on ammonia, nitrite, nitrate, and dissolved organic carbon in sterilization of seawater. Desalination Water Treat. 52(22-24), 4413-4422. https://doi.org/10.1080/19443994.2013.803702
  19. Meays, C. L., Boersma, K., Nordin, R., and Mazumder, A. 2004. Source tracking fecal bacteria in the water: a critical review of current methods. J. Environ. Manage. 73(1), 71-79. https://doi.org/10.1016/j.jenvman.2004.06.001
  20. Fong, T. T., and Lipp, E. K. 2005. Enteric viruses of humans and animals in aquatic environments: health risks, detection, and potential water quality assessment tools. Microbiol. Mol. Biol. Rev. 69(2), 357-371. https://doi.org/10.1128/MMBR.69.2.357-371.2005
  21. Garcia-Garay, J., Franco-Herera, A., and Machuca-Martinez, F.. 2020. Wild microorganism and plankton decay in ballast water treatments by solar disinfection (SODIS) and advanced oxidation process. Mar. Pollut. Bull. 154, 111060. https://doi.org/10.1016/j.marpolbul.2020.111060
  22. Standard method 9222 (Membrane filter technique for members of the colfiorm group), 2005. American Public Health Association (APHA), 21th edition.
  23. Environmental standard for water quality ES040701.1e (Total coliform-membrane filtration method). 2021, Ministry of Environment.
  24. Jnug, S., Kim, M., Kim, J., Choi, H., Kim, J., Do, J., Han, H., Kwon, M., Seo, J., Hwang, J., Kim, N., and Song, J. 2015. Guidelines for diagnosis of bacterial diseases of aquatic animals. National Institute of Fisheries Science.
  25. Jung, Y., Hong. E., Yoon, Y., Kwon, M., and Kang, J. W. 2014. Formation of bromate and chlorate during ozonation and electrolysis in seawater for ballast water. Ozone. Sci. Eng. 36(6), 515-525. https://doi.org/10.1080/01919512.2014.956862
  26. Kim, H., Lee, H., Kim, C. M., and Jang, A. 2020. Enhancement of ozonation of seawater-based wastewater containing pharmaceutical compounds by total residual oxidants: salinity, ammonia, and organic matter. Chemosphere. 259, 127513. https://doi.org/10.1016/j.chemosphere.2020.127513
  27. Reed, P., and Evans, R. 1981. Acute toxicity of chlorides, sulfates, and total dissolved solids to some fishes in Illinois. Illinois Department of Energy and Natural Resource (ENR).
  28. Hess-Erga, O. -K., Blomvagnes-Bakke, B., and Vadstein, O. 2010. Recolonization by hetero-trophic bacteria after UV irradiation or ozonation of seawater; a simulation of ballast water treatment. Water Res. 44(18), 5439-5449. https://doi.org/10.1016/j.watres.2010.06.059
  29. Becrerra-Castro, C., Macedo, G., Silva, A. M. T., Manaia, C. M., and Nunes, O. C. 2016. Proteobacteria become predominant during regrowth after water disinfection, Sci. Total Environ. 573, 313-323. https://doi.org/10.1016/j.scitotenv.2016.08.054
  30. Moreno-Andres, J., Acevedo-Merino, A., and Nebot, E. 2018. Study of marine bacteria inactivation by photochemical process: disinfection kinetics and growth modeling after treatment. Environ. Sci. Pollut. Res. 25(28), 27693-27703. https://doi.org/10.1007/s11356-017-1185-6