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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Advances in higher-order chromatin architecture: the move towards 4D genome

  • Jung, Namyoung (Department of Life Sciences, Pohang University of Science and Technology (POSTECH)) ;
  • Kim, Tae-Kyung (Department of Life Sciences, Pohang University of Science and Technology (POSTECH))
  • Received : 2021.02.19
  • Accepted : 2021.04.12
  • Published : 2021.05.31
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Abstract

In eukaryotes, the genome is hierarchically packed inside the nucleus, which facilitates physical contact between cis-regulatory elements (CREs), such as enhancers and promoters. Accumulating evidence highlights the critical role of higher-order chromatin structure in precise regulation of spatiotemporal gene expression under diverse biological contexts including lineage commitment and cell activation by external stimulus. Genomics and imaging-based technologies, such as Hi-C and DNA fluorescence in situ hybridization (FISH), have revealed the key principles of genome folding, while newly developed tools focus on improvement in resolution, throughput and modality at single-cell and population levels, and challenge the knowledge obtained through conventional approaches. In this review, we discuss recent advances in our understanding of principles of higher-order chromosome conformation and technologies to investigate 4D chromatin interactions.

Keywords

Acknowledgement

This work was funded by National Research Foundation of Korea (NRF) grants from the Korea government (MSIT) (NRF-2019R1A2C2006740, NRF-2019R1A5A6099645, NRF-2017M3A9G7073033, NRF-2019M3C7A1031537, and NRF-2020H1D3A1A04104610) (T.-K. K.).

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