Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant Effects of Ethanol Extracts of Aplidium pliciferum and Herdmania momus

만두멍게와 분홍멍게 에탄올 추출물의 항산화 효과

  • 강상모 (건국대학교 생물공학과) ;
  • 류정아 (건국대학교 생물공학과)
  • Received : 2021.03.22
  • Accepted : 2021.05.20
  • Published : 2021.05.28
Download PDF
⟨ Previous Next ⟩

Abstract

This study aims to verify the potential of functional cosmetic materials by examining the antioxidant effects of Aplidium pliciferum and Herdmania momus, used to determine the antioxidant efficacy of Aplidium pliciferum and Herdmania momus ethanol extracts, Mean and standard deviation were calculated using Minitab® 18 (IBM, USA). After various radicals were removed from the extracts of Aplidium pliciferum and Herdmania momus, DPPH was detected in 30.87±1.98% in Aplidium pliciferum, 88.27±0.84% in Herdmania momus, and ABTS in 56.23±2.50% in Aplidium pliciferum and 94.80±0.91% in Herdmania momus in the concentration of 20 mg/ml. Also, NO was detected in 88.57±0.81% in Aplidium pliciferum and 97.53±0.34% in Herdmania momus, respectively, thus all showing statistically significant differences. So we can confirm the efficiency of the solvent acts as an important factor through the total poly phenol content

본 연구는 해양자원인 만두멍게와 분홍멍게의 항산화 효과를 검증하여 기능성 화장품 소재 가능성을 확인하는데 목적이 있다. 본 연구에서는 만두멍게와 분홍멍게 에탄올 추출물의 항산화 효능을 확인하기 위하여 DPPH 소거능 및 ABTS 소거능, 폴리페놀 항산화 물질 함량, NO 생성 억제능 분석을 통하여 항산화 활성을 측정하기 위해서 항산화 실험을 진행하였다. 본 연구의 결과는 다음과 같다. 만두멍게와 분홍멍게의 추출물은 각종 radical(DPPH, ABTS, NO) 소거 효과 결과, 20 mg/ml 농도에서 DPPH는 만두멍게가 30.87±1.98%, 분홍멍게가 88.27±0.84%, ABTS는 만두멍게가 56.23±2.50%, 분홍멍게가 94.80±0.91%, NO는 만두멍게가 88.57±0.81%, 분홍멍게가 97.53±0.34%로 모두 유의한 차이가 있었다. 특히 총 폴리페놀 함량을 통해 분홍멍게의 항산화 물질 추출에 사용된 용매의 효율은 중요한 인자로 작용함을 확인하였다. 따라서 항산화 효과가 뛰어난 분홍멍게를 주물질로 첨가하여 만두멍게와 분홍멍게를 조합한 기능성 화장품 개발 가능성을 제시할 수 있다.

Keywords

References

  1. M. A. Cho, C. L. Park & C. J. Han. (2021). Effect of beauty lifestyle behaviors on the pursuit of beauty values and cosmetics purchasing behaviors. Journal of Convergence for Information Technology, 11(1), 261-267. https://doi.org/10.22156/CS4SMB.2021.11.01.261
  2. Z. Rohmah, A. A. Rofiqoh, S. H. Park & B. D. Choi. (2015). The Evaluation on the Effectiveness as a Cosmetic Material of Glycosaminoglycans Extracted from Halocynthia roretzi Tunic. Journal of Agriculture & Life Science, 49(3), 155-162. DOI : 10.14397/jals.2015.49.3.155
  3. T. F. Molinski, D. S. Dalisay, S. L. Lievens & J. P. Saludes. (2009). Drug development from marine natural products. Nature Reviews Drug Discovery, 8(1), 69-85. DOI : 10.1038/nrd2487
  4. J. I. Lee. (2007). Isolation and structure determination of bioactive constituents from the brown alga Sargassum siliquastrum. Master thesis, Korea Maritime and Ocean University of Korea.
  5. C. S. Kwak, S. A. Kim & M. S. Lee. (2005). The correlation of antioxidative effects of 5 Korean common edible seaweeds and total polyphenol content. Journal of the Korean Society of Food Science and Nutrition, 34(8), 1143-1150. https://doi.org/10.3746/jkfn.2005.34.8.1143
  6. S. H. Park. (2018). A study on the application of ascidian shell extract for cosmetic use and evaluation of its stability as a natural pigment. Master thesis, Mokwon University of Korea.
  7. J. K. Lee & H. G. Byun. (2015). Melanogensis inhibition effect of sea squirt. Proceedings of The Korean Society of Fisheries and Aquatic Science, 2015(5), 388-388.
  8. S. S. Kim, C. B. Ahn, S. W. Moon & J. Y. Je. (2018). Purification and antioxidant activities of peptides from sea squirt (Halocynthia roretzi) protein hydrolysates using pepsin hydrolysis. Food Bioscience, 25, 128-133. DOI : 10.1016/j.fbio.2018.08.010
  9. J. H. Park, B. Y. Seo, S. C. Lee & E. Park. (2010). Effects of ethanol extracts from stalked sea squirt (Styela clava) on antioxidant potential, oxidative DNA damage and DNA repair. Food Science and Biotechnology, 19(4), 1035-1040. DOI : 10.1007/s10068-010-0145-4
  10. M. A. Gunasinghe & S. M. Kim. (2018). Antioxidant and antidiabetic activities of vanadium binding proteins purified from the sea squirt Halocynthia roretzi. Journal of Food Science and Technology, 55(5), 1840-1849. DOI : 10.1007/s13197-018-3099-9
  11. C. Monmai, S. H. Go, I. S. Shin, S. G. You, H. Lee, S. B. Kang & W. J. Park. (2018). Immune-enhancement and anti-Inflammatory activities of fatty acids extracted from Halocynthia aurantium tunic in RAW264. 7 cells. Marine Drugs, 16(9), 309. DOI : 10.3390/md16090309
  12. Marine Bio-Resource Information System. (n. d.). Patent information. (Online). https://www.mbris.kr/pub/main/publicMainPage.do
  13. B. D. McKersie, J. Murnaghan, K. S. Jones & S. R. Bowley. (2000). Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. Plant Physiology, 122(4), 1427-1438. https://doi.org/10.1104/pp.122.4.1427
  14. T. Grasbon, E. M. Grasbon-Frodl, B. Juliusson, C. Epstein, P. Brundin, A. Kampik & B. Ehinger. (1999). CuZn superoxide dismutase transgenic retinal transplants. Graefe's Archive for Clinical and Experimental Ophthalmology, 237(4), 336-341. DOI : 10.1007/s004170050241
  15. M. S. Blois. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200. DOI : 10.1038/1811199a0
  16. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang & C. Rice-Evans. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. DOI : 10.1016/s0891-5849(98)00315-3
  17. O. Folin & W. Denis. (1912). On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological Chemistry, 12(2), 239-243. DOI : 10.1016/s0021-9258(18)88697-5
  18. T. Gutfinger. (1981). Polyphenols in olive oils. Journal of the American Oil Chemists' Society, 58(11), 966-968. DOI : 10.1007/bf02659771
  19. G. C. Jagetia, & M. S. Baliga. (2004). The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: a preliminary study. Journal of Medicinal Food, 7(3), 343-348. DOI : 10.1089/jmf.2004.7.343
  20. S. L. Shin & C. H. Lee. (2010). Antioxidant effects of the methanol extracts obtained from aerial part and rhizomes of ferns native to Korea. Korean Journal of Plant Resources, 23(1), 38-46.
  21. K. S. Lee, G. H. Kim, B. J. Seong, S. I. Kim, S. H. Han, S. S. Lee. & Y. C. Yoo. (2014). Anti-inflammatory activity of solvent fractions from ginseng berry extract in LPS-induced RAW264.7 cells. Korean Journal of Medical Crop Science, 22(6), 449-456. DOI : 10.7783/kjmcs.2014.22.6.449
  22. B. Ticar, Z. Rohmah, M. Bat-Erdene, S. H. Park & B. D. Choi. (2013). Evaluation of antioxidant and anti-inflammatory activities of ascidian tunic carotenoids as a source of color cosmetics. Korean Society for Biotechnology and Bioengineering Journal, 28(1), 36-41. DOI : 10.7841/ksbbj.2013.28.1.36
  23. M. S. Jung, G. S. Lee & H. J. Chae. (2004). In vitro biological activity assay of ethanol extract of radish. Applied Biological Chemistry, 47(1), 67-71.
  24. H. J. Kim, B. S. Jeon, S. K. Kim, J. Y. Cha& Y. S. Cho. (2000). Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). Journal of the Korean Society of Food Science and Nutrition, 29(6), 1127-1132.
  25. H. K. Kim, J. H. Kwon & N. Y. Park. (1998). Optimization of extraction conditions for ethanol extracts from Chrysanthemum morifolium by response surface methodology. Korean Journal of Food Science and Technology, 30(5), 1189-1196.
  26. J. Noh, Y. K. Choi, H. K. Kim & J. H. Kwon. (2005). Pie-establishment of microwave-assisted extraction conditions for antioxidative extracts from cabbage. Korean Journal of Food Preservation, 12(1), 62-67.
  27. J. T. Lee, C. E. Lee, J. H. Son, J. Y. Lee, T. S. Park, I. C. Lee & B. J. An. (2005). Cytotoxicity, antibacterial and antioxidant activities of the prescription Cheongyeolsodokum and its constituent herbs. The Korea Journal of Herbology, 20(4), 41-51.
  28. J. H. Kim, H. M. Kang, S. H. Lee, J. Y. Lee & L. Y. Park. (2015). Antioxidant and α-glucosidase inhibition activity of seaweed extracts. Korean J Food Preserv, 22, 290-296. https://doi.org/10.11002/kjfp.2015.22.2.290
  29. H. Bhaskar & N. Balakrishnan. (2009). In vitro antioxidant property of laticiferous plant species from Western Ghats Tamilnadu, India. International journal of health research, 2(2),
  30. Lakhanpal, P., & Rai, D. K. (2007). Quercetin: a versatile flavonoid. Internet Journal of Medical Update, 2(2), 22-37.