Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Occurrence of a Hybrid Between Taenia saginata and Taenia asiatica Tapeworms in Cambodia

  • Chang, Taehee (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Jung, Bong-Kwang (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Hong, Sooji (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Shin, Hyejoo (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Ryoo, Seungwan (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Lee, Jeonggyu (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Lee, Keon Hoon (Institute of Parasitic Diseases, Korea Association of Health Promotion) ;
  • Park, Hansol (Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Eom, Keeseon S. (Department of Parasitology and Medical Research Institute, Chungbuk National University College of Medicine) ;
  • Khieu, Virak (National Center for Parasitology, Entomology and Malaria Control, Ministry of Health) ;
  • Huy, Rekol (National Center for Parasitology, Entomology and Malaria Control, Ministry of Health) ;
  • Sohn, Woon-Mok (Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine) ;
  • Chai, Jong-Yil (Institute of Parasitic Diseases, Korea Association of Health Promotion)
  • Received : 2021.02.16
  • Accepted : 2021.03.17
  • Published : 2021.04.30
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Abstract

Human infection with Taenia asiatica or a hybrid between Taenia saginata and T. asiatica has not been reported in Cambodia. We detected for the first time a hybrid form between T. saginata and T. asiatica in Preah Vihear Province, Cambodia. An adult tapeworm specimen, i.e., 75 cm long strobila without scolex, was expelled from a 27-year-old man after praziquantel medication and purging. It was morphologically indistinguishable between T. saginata and T. asiatica. Several proglottids were molecularly analyzed to confirm the tapeworm species. The mitochondrial gene encoding cytochrome c oxidase subunit 1 (cox1) and nuclear genes encoding elongation factor-1α (ef1) and ezrin-radixin-moesin (ERM)-like protein (elp) were sequenced, and a single-allele analysis was performed to confirm the haploid genotype. The results revealed that our sample showed a discrepancy between the mitochondrial and 2 nuclear genes. It possessed homozygous sequences typical of T. saginata at cox1 and ef1 loci. However, it was heterozygous at the elp locus, with 1 allele in T. asiatica (elpA) and 1 in T. saginata (elpC), which indicates that it is a hybrid between T. saginata and T. asiatica. The present results confirmed the presence of a hybrid between T. saginata and T. asiatica in Cambodia and strongly suggest the existence of also 'pure' T. asiatica in Cambodia.

Keywords

Acknowledgement

We appreciate the staff in the National Center for Parasitology, Entomology and Malaria Control, Ministry of Health, Phnom Penh, Cambodia, who helped this survey. This work was supported by the Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul, South Korea.

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