대한방사선기술학회지:방사선기술과학 (Journal of radiological science and technology)

  • 제44권2호
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  • Pages.141-146
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  • 2021
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  • 2288-3509(pISSN)
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  • 2384-1168(eISSN)
대한방사선과학회 (Korean Society of Radiological Science)
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다이오드 레이저를 이용한 칸디다 알비칸스에 대한 항균 광역학 요법

Antimicribial Photodynamic Therapy Using Diode Laser on Candida Albicans

  • Kim, Ji-Won (Department of Radiological Science, Jeonju University) ;
  • Koo, Bon-Yeoul (Department of Radiology, Yeong dong Hospital)
  • 투고 : 2021.02.08
  • 심사 : 2021.03.31
  • 발행 : 2021.04.30
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초록

This study aimed to explore whether photodynamic therapy using Radachlorin and diode laser is an effective inhibitor of Candida albicans. Suspensions of Candida albicans were obtained, inoculated in petri dishes with Radachlorin, and incubated for 30 min. Then, the laser light of a diode laser was irradiated at at energy densities of 3 J/cm2, 5 J/cm2, 7 J/cm2. As a result, Candida albicans showed a killing rate of 91.5% at an energy density of 7 J/cm2. This study found that photodynamic therapy using a Radachlorin and diode laser was effective for the inhibition of Candida albicans.

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참고문헌

  1. Souza RC, Junqueira JC, Rossoni RD, Pereira CA, Munin E, Jorge AO. Comparison of the photodynamic fungicidal efficacy of methylene blue, toluidine blue, malachite green and low-power laser irradiation alone against Candida albicans. Lasers Med Sci. 2010;25(3):385-9. https://doi.org/10.1007/s10103-009-0706-z
  2. Rodrigues GB, Dias-Baruffi M, Holman N, Wainwright M, Braga GU. In vitro photodynamic inactivation of Candida species and mouse fibroblasts with phenothiazinium photosensitisers and red light. Photodiagnosis Photodyn Ther. 2013;10(2):141-9. https://doi.org/10.1016/j.pdpdt.2012.11.004
  3. Kim J, Sudbery P. Candida albicans, a major human fungal pathogen. J Microbiol. 2011;49(2):171-7. https://doi.org/10.1007/s12275-011-1064-7
  4. Ruhnke M, Rickerts V, Cornely OA, Buchheidt D, Glockner A, Heinz W, et al. Diagnosis and therapy of Candida infections: Joint recommendations of the German Speaking Mycological Society and the Paul-Ehrlich-Society for Chemotherapy. Mycoses. 2011;54(4):279-310. https://doi.org/10.1111/j.1439-0507.2011.02040.x
  5. Seo MG, Ahn GJ, Roh BI. Superficial cutaneous candidiasis. Of the Fungus. 1999;4:98-103.
  6. Rex JH, Rinaldi MG, Pfaller MA. Resistance of Candida species to fluconazole. Antimicrob Agents Chemother. 1995;39(1):1-8. https://doi.org/10.1128/AAC.39.1.1
  7. Oleinick NL, Evans HH. The photobiology of photodynamic therapy: Cellular targets and mechanisms. Radiat Res. 1998;150(5 Suppl):S146-56. https://doi.org/10.2307/3579816
  8. Kwon PS. Antimicrobial effects of photodynamic therapy using blue light emitting diode with photofrin and radachlorine against Propionibacterium acnes. Korean Journal of Clinical Laboratory Science. 2015;47(1):6-10. https://doi.org/10.15324/kjcls.2015.47.1.6
  9. Lee Y, Baron ED. Photodynamic therapy: Current evidence and applications in dermatology. Semin Cutan Med Surg. 2011;30(4):199-209. https://doi.org/10.1016/j.sder.2011.08.001
  10. Allison RR, Moghissi K. Photodynamic Therapy(PDT): PDT mechanisms. Clin Endosc. 2013;46(1):24-9. https://doi.org/10.5946/ce.2013.46.1.24
  11. Castano AP, Demidova TN, Hamblin MR. Mechanisms in photodynamic therapy: Part one-photosensitizers, photochemistry and cellular localization. Photodiagnosis and Photodynamic Therapy. 2004;1(4):279-93. https://doi.org/10.1016/S1572-1000(05)00007-4
  12. Vahabi S, Fekrazad R, Ayremlou S, Taheri S, Zangeneh N. The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on streptococcus mutans: An in vitro study. J Dent (Tehran). 2011;8(2):48-54.
  13. Choi YD, Yoon SG, Chea MJ. Cancer imaging and photodynamic therapy using photosensitizers. Polymer Science and Technology. 2008;19(2):138-45.
  14. Moslemi N, Soleiman-Zadeh Azar P, Bahador A, Rouzmeh N, Chiniforush N, Paknejad M, et al. Inactivation of Aggregatibacter actinomycetemcomitans by two different modalities of photodynamic therapy using Toluidine blue O or Radachlorin as photosensitizers: An in vitro study. Lasers Med Sci. 2015;30(1):89-94. https://doi.org/10.1007/s10103-014-1621-5
  15. Fujishima I, Sakai T, Tanata T, Ryu H, Uemura K, Fujishima Y, et al. Photodynamic therapy using pheophorbide a and Nd: YAG laser. Neurologia medico-chirurgica. 1991;31(5):257-63. https://doi.org/10.2176/nmc.31.257
  16. Hamblin MR, O'Donnell DA, Murthy N, Contag CH, Hasan T. Rapid control of wound infections by targeted photodynamic therapy monitored by in vivo bioluminescence imaging. Photochem Photobiol. 2002;75(1):51-7. https://doi.org/10.1562/0031-8655(2002)075<0051:RCOWIB>2.0.CO;2
  17. Giroldo LM, Felipe MP, De Oliveira MA, Munin E, Alves LP, Costa MS. Photodynamic antimicrobial chemotherapy(PACT) with methylene blue increases membrane permeability in Candida albicans. Lasers Med Sci. 2009;24(1):109-12. https://doi.org/10.1007/s10103-007-0530-2
  18. Capella M, Coelho AM, Menezes S. Effect of glucose on photodynamic action of methylene blue in Escherichia coli cells. Photochem Photobiol. 1996; 64(1):205-10. https://doi.org/10.1111/j.1751-1097.1996.tb02443.x
  19. Mima EG, Pavarina AC, Dovigo LN, Vergani CE, Costa CA, Kurachi C, et al. Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109(3):392-401. https://doi.org/10.1016/j.tripleo.2009.10.006
  20. Lim HS. Reduction of thermal damage in photodynamic therapy by laser irradiation techniques. J Biomed Opt. 2012;17(12):128001. https://doi.org/10.1117/1.JBO.17.12.128001
  21. Fekrazad R, Zare H, Mohammadi Sepahvand S, Morsali P. The effect of antimicrobial photodynamic therapy with radachlorin(R) on Staphylococcus aureus and Escherichia coli: An in vitro study. J Lasers Med Sci. 2014;5(2):82-5.
  22. Fekrazad R, Zare H, Vand SM. Photodynamic therapy effect on cell growth inhibition induced by Radachlorin and toluidine blue O on Staphylococcus aureus and Escherichia coli: An in vitro study. Photodiagnosis Photodyn Ther. 2016;15:213-7. https://doi.org/10.1016/j.pdpdt.2016.07.001
  23. Moslemi N, Rouzmeh N, Shakerinia F, Bahador A, Soleimanzadeh Azar P, Kharazifard MJ, et al. Photodynamic inactivation of porphyromonas gingivalis utilizing radachlorin and toluidine blue O as photosensitizers: An in vitro study. J Lasers Med Sci. 2018;9(2):107-12. https://doi.org/10.15171/jlms.2018.21
  24. Queiroga AS, Trajano VN, Lima EO, Ferreira AF, Queiroga AS, Limeira Jr. FA. In vitro photodynamic inactivation of Candida spp. by different doses of low power laser light. Photodiagnosis Photodyn Ther. 2011;8(4):332-6. https://doi.org/10.1016/j.pdpdt.2011.08.005
  25. Teichert MC, Jones JW, Usacheva MN, Biel MA. Treatment of oral candidiasis with methylene blue-mediated photodynamic therapy in an immunodeficient murine model. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;93(2):155-60. https://doi.org/10.1067/moe.2002.120051
  26. Dovigo LN, Pavarina AC, Ribeiro DG, Adriano CS, Bagnato VS. Photodynamic inactivation of four Candida species induced by photogem((R)). Braz J Microbiol. 2010;41(1):42-9. https://doi.org/10.1590/S1517-83822010000100009