융합정보논문지 (Journal of Convergence for Information Technology)

중소기업융합학회 (Convergence Society for SMB)
권호 표지
DOI QR코드

DOI QR Code

유령멍게(Ciona intestinalis)와 노랑꼭지유령멍게(Ciona savignyi) 에탄올 추출물의 항산화 효과

The Antioxidation Effect of Ethanol Extracts from Ciona intestinalis and Ciona savignyi

  • 이정은 (건국대학교 대학원 생물공학과) ;
  • 강상모 (건국대학교 생물공학과)
  • Lee, Jeong-Eun (Dept. of Biological Engineering, Graduate School, Konkuk University) ;
  • Kang, Sang-Mo (Dept. of Biological Engineering, Konkuk University)
  • 투고 : 2021.03.15
  • 심사 : 2021.04.20
  • 발행 : 2021.04.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

유령멍게와 노랑꼭지유령멍게는 국내의 경우, 항만, 선박의 바닥, 양식장에 대량으로 발생하여 큰 피해를 입혀, 위해종으로 지정되어 있다. 따라서 이들을 이용하는 측면에서 화장품 원료로의 사용 가능성을 검토하였다. 유령멍게와 노랑꼭지유령멍게를 70% 에탄올로 추출물을 제조하여 항산화능을 측성하였다. 유령멍게와 노랑꼭지유령멍게 추출물 20 mg/mL에서 DPPH는 각각 71.80%, 21.40%를 보였고, ABTS는 각각 95.47%, 27.53%, nitric oxide 생성 억제능은 각각 97.47%, 88.90%로 나타났다. 총 페놀 함량은 유령멍게와 노랑꼭지유령멍게에서 각각 0.081, 0.041 mg gallic acid/mg extract으로 나타났다. 결과적으로 노랑꼭지유령멍게보다 유령멍게에서 다소 높은 항산화능을 보였다. 이들은 추출물은 동물성 유래 항산화물질들로 화장품 소재로서 활용 가능함을 확인하였다.

Ciona intestinalis and Ciona savignyi have been designated as hazardous species because they have caused great damages as they appeared in ports, ships, and farms in Korea in large amount. Therefore, in terms of using them, the possibility of use as cosmetic ingredients was examined. C. intestinalis and C. savignyi were treated with 70% ethanol to make an extract and then their antioxidant activity was measured. C. intestinalis and C. savignyi showed 71.80% and 21.40% of DPPH at 20mg/mL of extract, respectively. ABTS was 95.47% and 27.53%, while nitric oxide production inhibitory ability was 97.47% and 88.90%, respectively. Total phenol content was 0.081 and 0.041 mg gallic acid/mg extract, respectively. As a result, C. intestinalis showed somewhat higher antioxidant activity than C. savignyi. It is confirmed that these extracts are animal-derived antioxidants that can be used as cosmetic materials.

키워드

참고문헌

  1. J. H. Choi, J. H. Yeum & D. K. Bae. (2009). Utilization of natural plant pigments resource. Fiber Technology and Industry, 13, 113-121.
  2. Z. Rohmah, A. A. Rofiqoh, S. H. Park & B. D. Choi. (2015). The Evaluation on the Effectiveness as a Cosmetic Material of Glycosaminoglycans Extracted from Halocynthia roretzi Tunic. Journal of Agriculture & Life Sciences, 49(3), 155-162. DOI : 10.14397/jals.2015.49.3.155
  3. Jo, J.-E., Kim, K.-H., Yoon, M.-H., Kim, N.-Y., Lee, C., & Yook, H.-S. (2010). Quality Characteristics and Antioxidant Activity Research of Halocynthia roretzi and Halocynthia aurantium. Journal of the Korean Society of Food Science and Nutrition, 39(10), 1481-1486. DOI : 10.3746/jkfn.2010.39.10.1481
  4. S. H. Park, S. B. Jeong, & B. D. Choi. (2013). Effects of Halocynthia roretzi bark extract on immune activity and cancer cell growth. Journal of Agriculture & Life Science, 47 (5), 163-170.
  5. B. Ticar, Z. Rohmah, M. Bat-Erdene, S.-H. Park & B. D. Choi. (2013). Evaluation of Antioxidant and Anti-Inflammatory Activities of Ascidian Tunic Carotenoids As a Source of Color Cosmetics. KSBB Journal, 28(1), 36-41. DOI : 10.7841/ksbbj.2013.28.1.36
  6. F. Kocak, Z. Ergen & M. E. Cinar. (1999). Fouling organisms and their developments in a polluted and an unpolluted marina in the Aegean Sea (Turkey). Ophelia, 50(1), 1-20. DOI : 10.1080/00785326.1999.10409385
  7. D. G. Kim, J. U. Park, D. H. Kim, T. J. Yoon & S. Shin, (2017). The Effect of temperature on early growth of Ciona intestinalis (Ascidiacea, Phlebobranchia, Cionidae). Environmental Biology Research, 35(1), 1-5. DOI : 10.11626/kjeb.2017.35.1.001
  8. S. Y. Hong. (2006). Korean Marine Invertebrates. Seoul : Academy books
  9. C. E. Carver, A. L. Mallet & B. Vercaemer. (2006). Biological synopsis of the solitary tunicate Ciona intestinalis (p.52). Dartmouth, Nova Scotia: Bedford Institute of Oceanography.
  10. A. Locke &M. Carman. (2009). Ecological interactions between the vase tunicate (Ciona intestinalis) and the farmed blue mussel (Mytilus edulis) in Nova Scotia, Canada. Aquatic Invasions, 4(1), 177-187. https://doi.org/10.3391/ai.2009.4.1.18
  11. H. J. Park, W. G. Park, J. W. Choi & B. R. Lee. (2017). Variation of Community Structure of Decapods by Season and Depth near Oryuk Islets off Busan, Korea. Journal of Fisheries and Marine Sciences Education, 29(1), 257-269. DOI : 10.13000/JFMSE.2017.29.1.257
  12. L. F. Capitan-Vallvey, M. C. Valencia & E. A. Nicolas. (2001). Monoparameter sensors for the determination of the antioxidants butylated hydroxyanisole and n-propyl gallate in foods and cosmetics by flow injection spectrophotometry. Analyst, 126(6), 897-902. DOI : 10.1039/B101162F
  13. J. M. Gillbro & M. J. Olsson, (2011). The melanogenesis and mechanisms of skin-lightening agents-existing and new approaches. International Journal of cosmetic science, 33(3), 210-221. DOI : 10.1111/j.1468-2494.2010.00616.x
  14. H. Masaki. (2010). Role of antioxidants in the skin: anti-aging effects. Journal of dermatological science, 58(2), 85-90. DOI : 10.1016/j.jdermsci.2010.03.003
  15. C. Lee, J. H. Jang, S. C. Kim, J. W. Chung & C. I. Park. (2010). Protective Effect of Marine Natural Products against UVB-induced Damages in Human Skin Fibroblast via Antioxidant Mechanism. journal of the society of cosmetic scientists of korea, 36(1), 79-87.
  16. D. Harman. (1995). Free radical theory of aging: Alzheimer's disease pathogenesis. Age, 18(3), 97-119. https://doi.org/10.1007/BF02436085
  17. L. Rittie & G. J. Fisher. (2002). UV-light-induced signal cascades and skin aging. Ageing research reviews, 1(4), 705-720. DOI : 10.1016/S1568-1637(02)00024-7
  18. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang & C. Rice-Evans. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. DOI : 10.1016/S0891-5849(98)00315-3
  19. Y. Kambayashi, N. T. Binh, H. W. Asakura, Y. Hibino, Y. Hitomi, H. Nakamura & K. Ogino. (2009). Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate. Journal of clinical biochemistry and nutrition, 44(1), 46-51. DOI : 10.3164/jcbn.08-162
  20. J. H. Song & S. R. Lee. (2015). Anti-oxidant and Inhibitory Activity on NO Production of Extract and its Fractions from Rosa davurica Pall. Leaves. Korean Journal of Medicinal Crop Science, 23(1), 20-26. DOI: 10.7783/kjmcs.2015.23.1.20
  21. Y. K. Park & J. H. Kim. (2016). Antioxidant activity, total phenolic content, vitamin C content, and sugar content according to maturity level of jujube (Zyziphus jujuba) varieties. The Plant Resources Society of Korea, 29(5), 539-546. DOI : 10.7732/kjpr.2016.29.5.539
  22. S. H. Jang, E. A. Yu, K. S. Han, S. C. Shin, H. K. Kim & S. G. Lee. (2008). Research Reports : Changes in Total Polyphenol Contents and DPPH Radical Scavenging Activity of Agrimonia pilosa According to Harvest Time and Various Part. Korean Journal of Medicinal Crop Science, 16(6), 397-401.