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Simultaneous determination of phytochemical constituents in Paeonia lactiflora extracts using the HPLC-UV method

  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kang, Sam Sik (Natural Products Research Institute and College of Pharmacy, Seoul National University) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2020.12.16
  • Accepted : 2020.12.28
  • Published : 2021.03.31

Abstract

Qantitative analysis of six compounds: (+)-catechin, benzoic acid, gallic acid methyl ester, paeonol, paeoniflorin, and albiflorin from Paeonia lactiflora extracts was performed using high-performance liquid chromatography and an ultraviolet (UV) detector, following different extraction methods. A reverse-phase column was used in a gradient elution system, and UV detection was performed at 280 nm. The results showed that the quantity of paeoniflorin was the highest in ethanol and water extracts (73.89 and 57.87 mg/g, respectively) among the six compounds. This study contributes a good analysis method for the contents of P. lactiflora and would be propitious for developing medicines and functional foods.

Keywords

References

  1. Lee TB (1985) Colored Flora of Korea I. Hyangmoon Press, Seoul
  2. Zhang W, Dai S (2012) Mechanisms involved in the therapeutic effects of Paeonia lactiflora Pallas in rheumatoid arthritis. Int Immunopharmacol 14: 27-31 https://doi.org/10.1016/j.intimp.2012.06.001
  3. Wu SH, Wu DG, Chen YW (2010) Chemical constituents and bioactivities of plants from the genus Paeonia. Chem Biodivers 7: 90-104 https://doi.org/10.1002/cbdv.200800148
  4. Goto H, Shimada Y, Akechi Y, Kohta K, Hattori M, Terasawa K (1996) Endothelium-dependent vasodilator effect of extract prepared from the roots of Paeonia lactiflora on isolated rat aorta. Planta Med 62: 436-439 https://doi.org/10.1055/s-2006-957934
  5. Li J, Chen CX, Shen YH (2011) Effects of total glucosides from paeony (Paeonia lactiflora Pall) roots on experimental atherosclerosis in rats. J Ethnopharmacol 135: 469-475 https://doi.org/10.1016/j.jep.2011.03.045
  6. Zhou JX, Braun MS, Wetterauer P, Wetterauer B, Wink M (2019) Antioxidant, cytotoxic, and antimicrobial activities of Glycyrrhiza glabra L., Paeonia lactiflora Pall., and Eriobotrya japonica (Thunb.) Lindl. extracts. Medicines (Basel) 6: 43 https://doi.org/10.3390/medicines6020043
  7. Braca A, Kiem PV, Yen PH, Nhiem NX, Quang TH, Cuong NX, Minh CV (2008) New monoterpene glycosides from Paeonia lactiflora. Fitoterapia 79: 117-120 https://doi.org/10.1016/j.fitote.2007.11.001
  8. Wang HB, Gu WF, Chu WJ, Zhang S, Tang XC, Qin GW (2009) Monoterpene glucosides from Paeonia lactiflora. J Nat Prod 72: 1321-1324 https://doi.org/10.1021/np8001783
  9. Kamiya K. Yoshioka K, Saiki Y, Ikuta A, Satake T (1997) Triterpenoids and flavonoids from Paeonia lactiflora. Phytochemistry 44: 141-144 https://doi.org/10.1016/S0031-9422(96)00518-3
  10. Ikuta A, Kamiya K, Satake T, Saiki Y (1995) Triterpenoids from callus tissue cultures of Paeonia species. Phytochemistry 38: 1203-1207 https://doi.org/10.1016/0031-9422(94)00445-Y
  11. Kumar N, Motto MG (1985) Volatile constituents of peony flowers. Phytochemistry 25: 250-253 https://doi.org/10.1016/S0031-9422(00)94541-2
  12. Tanaka T, Fukumori M, Ochi T, Kouno I (2003) Paeonianins A-E, new dimeric and monomeric ellagitannins from the fruits of Paeonia lactiflora. J Nat Prod 66: 759-763 https://doi.org/10.1021/np020608g
  13. Kim HJ, Chang EJ, Bae SJ, Shim SM, Park HD, Rhee CH, Park JH, Choi SW (2002) Cytotoxic and antimutagenic stilbenes from seeds of Paeonia lactiflora. Arch Pharm Res 25: 293-299 https://doi.org/10.1007/BF02976629
  14. Jia N, Shu QY, Wang LS, Du H, Xu YJ, Liu ZA (2008) Analysis of petal anthocyanins to investigate coloration mechanism in herbaceous peony cultivars. Sci Hortic 117: 167-173 https://doi.org/10.1016/j.scienta.2008.03.016
  15. Guo D, Ye G, Guo H (2006) A new phenolic glycoside from Paeonia lactiflora. Fitoterapia 77: 613-614 https://doi.org/10.1016/j.fitote.2006.05.026
  16. Kang SS, Kim JS, Yun-Choi HS, Han BH (1993) Phytochemical studies on Paeonia radix. Kor J Pharmacogn 24: 247-250
  17. Choung MG, Kang KH (1997) Isolation and determination of paeoniflorin and albiflorin in Korea peony (Paeonia lactiflora Pall) root. Korean J Medicinal Crop Sci 5: 249-254
  18. He DY, Dai SM (2011) Anti-inflammatory and immunomodulatory effects of Paeonia lactiflora Pall., a traditional Chinese herbal medicine. Front Pharmacol 2: 10 https://doi.org/10.3389/fphar.2011.00010
  19. Abdel-Hafez AA, Meselhy MR, Nakamura N, Hattori M, Watanabe H, Murakami Y, El-Gendy MA, Mahfouz NM, Mohamed TA (1999) Anticonvulsant activity of paeonimetabolin-I adducts obtained by incubation of paeoniflorin and thiol compounds with Lactobacillus brevis. Biol Pharm Bull 22: 491-197 https://doi.org/10.1248/bpb.22.491
  20. Hsu FL, Lai CW, Cheng JT (1997) Antihyperglycemic effects of paeoniflorin and 8-debenzoylpaeoniflorin, glucosides from the root of Paeonia lactiflora. Planta Med 63: 323-325 https://doi.org/10.1055/s-2006-957692
  21. Wang QS, Gao T, Cui YL, Gao LN, Jiang HL (2014) Comparative studies of paeoniflorin and albiflorin from Paeonia lactiflora on antiinflammatory activities. Pharm Biol 52: 1189-1195 https://doi.org/10.3109/13880209.2014.880490
  22. Zhou J, Wang L, Wang J, Wang C, Yang Z, Wang C, Zhu Y, Zhang J (2016) Paeoniflorin and albiflorin attenuate neuropathic pain via MAPK pathway in chronic constriction injury rats. Evid Based Complement Alternat Med 2016: Article ID 8082753 (11 pages)
  23. Suh KS, Choi EM, Lee YS, Kim YS (2013) Protective effect of albiflorin against oxidative-stress-mediated toxicity in osteoblast-like MC3T3-E1 cells. Fitoterapia 89: 33-41 https://doi.org/10.1016/j.fitote.2013.05.016
  24. Wang YL, Wang JX, Hu XX, Chen L, Qiu ZK, Zhao N, Yu ZD, Sun SZ, Xu YY, Guo Y, Liu C, Zhang YZ, Li YF, Yu CX (2016) Antidepressantlike effects of albiflorin extracted from Radix Paeoniae Alba. J Ethnopharmacol 179: 9-15 https://doi.org/10.1016/j.jep.2015.12.029
  25. Heo JI, Kim JH, Lee JM, Kim SC, Park JB, Kim J, Lee JY (2013) Antioxidant activity and its mechanism of Paeonia lactiflora Pall extract. Nat Prod Sci 19: 49-53
  26. Lee S, Ji S (2004) Effect of Paeonia lactiflora extracts on α-glucosidase. Nat Prod Sci 10: 223-227
  27. Park KD, Cho SH (2010) Antimicrobial characteristics of Paeonia lactiflora Pall. extract tested against food-putrefactive microorganisms. Korean J Food Preserv 17: 706-711
  28. Yang HO, Ko WK, Kim JY, Ro HS (2004) Paeoniflorin: an antihyperlipidemic agent from Paeonia lactiflora. Fitoterapia 75: 45-49 https://doi.org/10.1016/j.fitote.2003.08.016
  29. Qiu J, Chen M, Liu J, Huang X, Chen J, Zhou L, Ma J, Sextius P, Pena AM, Cai Z, Jeulin S (2016) The skin-depigmenting potential of Paeonia lactiflora root extract and paeoniflorin: in vitro evaluation using reconstructed pigmented human epidermis. Int J Cosmet Sci 38: 444-451 https://doi.org/10.1111/ics.12309
  30. Choung MG, Kang KH (1994) Extraction methods and HPLC analysis conditions of paeoniflorin in peony, Paeonia lactiflora Pall. Korean J Crop Sci 39: 542-547
  31. Kim TK, Kim KJ, Joo GJ, Rhee IK (1997) Changes of paeoniflorin content in peony roots by heat-treatmnt. Korean J Food Preserv 4: 69-75
  32. Kim TK, Joo GJ, Chung JD, Rhee IK (1996) Analysis of the content of paeoniflorin in peony roots cultivated on kyeongbuk area. Agric Res Bull Kyungpook Natl Univ 14: 15-28
  33. Asakawa N, Hattori T, Ueyama M, Shinoda A, Miyake Y (1979) Determination of paeoniflorin in Paeony extract by high performance liquid chromatography. Yakugaku Zasshi 99: 598-601 https://doi.org/10.1248/yakushi1947.99.6_598
  34. Cho WG, Kyung KY, Yu SM (2009) Stability of paeoniflorin used as anti-wrinkle agents in emulsions. J Korean Oil Chem Soc 26: 191-198
  35. Qiu F, Zhong X, Mao Q, Huang Z (2013) The antidepressant-like effects of paeoniflorin in mouse models. Exp Ther Med 5: 1113-1116 https://doi.org/10.3892/etm.2013.925
  36. Xu X, Liu H, Pan Y, Liu Z, Chen D, Zhang L, Yuang H (2020) Albiflorin attenuates inflammation and apoptosis by upregulating AMPK-mediated expression of CDX2 in a mouse model of ulcerative colitis. Trop J Pharm Res 19: 995-999 https://doi.org/10.4314/tjpr.v19i5.13
  37. Zhu Y, Zhou J, Wang L, Yang Z, Zhang J (2015) Hematopoietic effects of paeoniflorin and albiflorin on radiotherapy and chemotherapy-induced anemia mice. Planta Med 81: 928
  38. Wang J, Wan Y, Zheng N (2020) Albiflorin ameliorates depressive-like behaviors in mice induced by chronic unpredictable mild stress. Curr Topics Nutraceut Res 18: 102-107