한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제49권4호
  • /
  • Pages.543-551
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  • 2021
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Anti-Inflammatory and Anti-Fibrotic Activities of Nocardiopsis sp. 13G027 in Lipopolysaccharides-Induced RAW 264.7 Macrophages and Transforming Growth Factor Beta-1-Stimulated Nasal Polyp-Derived Fibroblasts

  • Choi, Grace (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea) ;
  • Kim, Geum Jin (College of Pharmacy, Yeungnam University) ;
  • Choi, Hyukjae (College of Pharmacy, Yeungnam University) ;
  • Choi, Il-Whan (Department of Microbiology and Immunology, Inje University College of Medicine) ;
  • Lee, Dae-Sung (Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea)
  • 투고 : 2021.09.29
  • 심사 : 2021.11.16
  • 발행 : 2021.12.28
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초록

Nocardiopsis species produce bioactive compounds, such as antimicrobial and anti-cancer agents and toxins. However, no reports have described their anti-inflammatory and anti-fibrotic effects during nasal polyp (NP) formation. In this study, we investigated whether marine-derived bacterial Nocardiopsis sp. 13G027 exerts anti-inflammatory and anti-fibrotic effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and transforming growth factor (TGF)-β1-induced NP-derived fibroblasts (NPDFs). Nitric oxide (NO) and prostaglandin E2 (PGE2) levels were analyzed. Extract from Nocardiopsis sp. 13G027 significantly inhibited the upregulation of NO and PGE2 in LPS-activated RAW 264.7 macrophages. The expression of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt/PKB) in LPS-induced RAW 264.7 macrophages was evaluated; smooth muscle alpha-actin (α-SMA), collagen type I (Col-1), and fibronectin also phosphorylated small mothers against decapentaplegic (SMAD) 2 and 3 in TGF-β1-stimulated NPDFs. The Nocardiopsis sp. 13G027 extract suppressed the phosphorylation of MAPKs and Akt and the DNA-binding activity of activator protein 1 (AP-1). The expression of pro-fibrotic components such as α-SMA, Col-1, fibronectin, and SMAD2/3 was inhibited in TGF-β1-exposed NPDFs. These findings suggest that Nocardiopsis sp. 13G027 has the potential to treat inflammatory disorders, such as NP formation.

키워드

과제정보

This study was supported by grant (2021M00500) from the National Marine Biodiversity Institute of Korea. We would also like to thank researcher, Yun Gyeong Park who helped with the ABTS assay.

참고문헌

  1. Cho JS, Kang JH, Shin JM, Park IH, Lee HM. 2015. Inhibitory effect of delphinidin on extracellular matrix production via the MAPK/NF-kB pathway in nasal polyp-derived fibroblasts. Allergy Asthma Immunol. Res. 7: 276-282. https://doi.org/10.4168/aair.2015.7.3.276
  2. Pawankar R. 2003. Nasal polyposis: an update: editorial review. Curr. Opin. Allergy Clin. Immunol. 3: 1-6. https://doi.org/10.1097/00130832-200302000-00001
  3. Jung JW, Park IH, Cho JS, Lee HM. 2013. Naringenin inhibits extracellular matrix production via extracellular signal-regulated kinase pathways in nasal polyp-derived fibroblasts. Phytother. Res. 27: 463-467. https://doi.org/10.1002/ptr.4735
  4. Cho JS, Moon YM, Um JY, Moon JH, Park IH, Lee HM. 2012. Inhibitory effect of ginsenoside Rg1 on extracellular matrix production via extracellular signal-regulated protein kinase/activator protein 1 pathway in nasal polyp-derived fibroblasts. Exp. Biol. Med. (Maywood) 237: 663-669. https://doi.org/10.1258/ebm.2012.011342
  5. Bennur T, Kumar AR, Zinjarde S, Javdekar V. 2015. Nocardiopsis species: incidence, ecological roles and adaptations. Microbiol. Res. 174: 33-47. https://doi.org/10.1016/j.micres.2015.03.010
  6. Sun HH, White CB, Dedinas J, Cooper R, Sedlock DM. 1991. Methylpendolmycin an indolelactam from a Nocardiopsis sp. J. Nat. Prod. 54: 1440-1443. https://doi.org/10.1021/np50077a040
  7. Kim JW, Adachi H, Shin-ya K, Hayakawa Y, Seto H. 1997. Apoptolidin, a new apoptosis inducer in transformed cells from Nocardiopsis sp. J. Antibiot. 50: 628-630. https://doi.org/10.7164/antibiotics.50.628
  8. Li YQ, Li MG, Li W, Zhao JY, Ding ZG, Cui XL, et al. 2007. Griseusin D, a new pyranonaphthoquinone derivative from a alkaphilic Nocardiopsis sp. J. Antibiot. 60: 757-761. https://doi.org/10.1038/ja.2007.100
  9. Gandhimathi R, Kiran GS, Hema TA, Selvin J, Raviji TR, Shanmughapriya S. 2009. Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10. Bioprocess Biosyst. Eng. 32: 825-835. https://doi.org/10.1007/s00449-009-0309-x
  10. Engelhardt K, Degnes KF, Kemmler M, Bredholt H, Fjaervik E, Klinkenberg G, et al. 2010. Production of a new thiopeptide antibiotic, TP-1161, by a marine Nocardiopsis species. Appl. Environ. Micobiol. 76: 4969-4976. https://doi.org/10.1128/AEM.00741-10
  11. Ding ZG, Li MG, Zhao JY, Ren J, Huang R, Xie MJ, et al. 2010. Naphthospironone A: an unprecedented and highly functionalized polycyclic metabolite from an alkalinemine waste extremophile. Chemistry 16: 3902-3905. https://doi.org/10.1002/chem.200903198
  12. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
  13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  14. Schmolz L, Wallert M, Lorkowski S. 2017. Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay. J. Immunol. Methods 449: 68-70. https://doi.org/10.1016/j.jim.2017.06.012
  15. Lee DS, Lee CM, Park SK, Yim MJ, Lee JM, Choi G, et al. 2017. Anti-inhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts. Int. J. Mol. Med. 40: 1950-1956.
  16. Sharma JN, Al-Omran A, Parvathy SS. 2007. Role of nitric oxide in inflammatory diseases. Inflammophamacology 15: 252-259. https://doi.org/10.1007/s10787-007-0013-x
  17. Ruhee RT, Ma S, Suzuki K. 2019. Sulforaphane protects cells against lipopolysaccharide-stimulated inflammation in murine macrophages. Antioxidants 8: 577. https://doi.org/10.3390/antiox8120577
  18. Kwon DH, Cha HJ, Choi EO, Leem SH, Kim GY, Moon SK, et al. 2018. Schisandrin A suppresses lipopolysaccharide-induced inflammation and oxidative stress in RAW 264.7 macrophages by suppressing the NF-kB, MAPKs and PI3K/Akt pathways and activating Nrf2/HO-1 signaling. Int. J. Mol. Med. 41: 264-274. https://doi.org/10.3892/ijmm.2017.3209
  19. Zong Y, Sun L, Liu B, Deng YS, Zhan D, Chen YL, et al. 2012. Resveratrol inhibits LPS-induced MAPKs activation via activation of the phosphatidylinositol 3-kinase pathway in murine RAW 264.7 macrophage cells. PLoS One 7: e44107. https://doi.org/10.1371/journal.pone.0044107
  20. Guha M, Mackman N. 2001. LPS induction of gene expression in human monocytes. Cell. Signal. 13: 85-94. https://doi.org/10.1016/S0898-6568(00)00149-2
  21. Hu W, Wang X, Wu L, Shen T, Ji L, Zhao X, et al. 2016. Apigenin-7-O-β-D-glucuronide inhibits LPS-induced inflammation through the inactivation of AP-1 and MAPK signaling pathways in RAW 264.7 macrophages and protects mice against endotoxin shock. Food Funct. 7: 1002-1013. https://doi.org/10.1039/C5FO01212K
  22. Li X, Shen J, Jiang Y, Shen T, You L, Sun X, et al. 2016. Anti-inflammatory effects of chloranthalactone B in LPS-stimulated RAW 264.7 cells. Int. J. Mol. Sci. 17: 1938. https://doi.org/10.3390/ijms17111938
  23. Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, et al. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules 21: 1374. https://doi.org/10.3390/molecules21101374
  24. Xu X, Li H, Hou X, Li D, He S, Wan C, et al. 2015. Punicalagin induces Nrf2/HO-1 expression via upregulation of PI3K/AKT pathway and inhibits LPS-induced oxidative stress in RAW 264.7 macrophages. Mediat. Inflamm. 2015: 380218. https://doi.org/10.1155/2015/380218
  25. Kaminska B. 2005. MAPK signaling pathways as molecular targets for anti-inflammatory therapy-from molecular mechanisms to therapeutic benefits. Biochem. Biophys. Acta 1754: 253-262.
  26. Williams D, Li C, Ha T, Ozment-Skelton T, Kalbfleisch JH, Preiszner J, et al. 2004. Modulation of the phosphoinositide 3-kinase pathway alters innate resistance to polymicrobial sepsis. J. Immunol. 172: 449-456. https://doi.org/10.4049/jimmunol.172.1.449
  27. Salaria N, Sharma N, Garg U, Saluja SK, Agarwal R. 2015. Inflammatory septal nasal polyp. Iran J. Otorhinolaryngol. 27: 319-323.
  28. Wick G, Backovic A, Rabensteiner E, Plank N, Schwentner C, Sgonc R. 2010. The immunology of fibrosis: Innate and adaptive responses. Trends Immunol. 31: 110-119. https://doi.org/10.1016/j.it.2009.12.001
  29. Park SK, Jin YD, Park YK, Yeon SH, Xu J, Han RN, et al. 2017. IL-25-induced activation of nasal fibroblast and its association with the remodeling of chronic rhinosinusitis with nasal polyposis. PLoS One 12: e0181806. https://doi.org/10.1371/journal.pone.0181806
  30. Shoulders MD, Raines RT. 2009. Collagen structure and stability. Annu. Rev. Biochem. 78: 929-958. https://doi.org/10.1146/annurev.biochem.77.032207.120833
  31. Ryu NH, Shin JM, Um JY, Park IH, Lee HM. 2016. Wogonin inhibits transforming growth factor beta1-induced extracellular matrix production via the p38/activator protein 1 signaling pathway in nasal polyp-derived fibroblasts. Am. J. Rhinol. Allergy 30: 128-133. https://doi.org/10.2500/ajra.2016.30.4329
  32. Little SC, Early SB, Woodard CR, Shonka Jr. DC, Han JK, Borish L, et al. 2008. Dual action of TGF-beta1 on nasal-polyp derived fibroblasts. Laryngoscope 118: 320-324. https://doi.org/10.1097/MLG.0b013e318159cc0b
  33. Park IH, Park SJ, Cho JS, Moon YM, Kim TH, Lee SH, et al. 2012. Role of reactive oxygen species in transforming growth factor beta1-induced alpha smooth-muscle actin and collagen production in nasal polyp-derived fibroblasts. Int. Arch. Allergy Immunol. 159: 278-286. https://doi.org/10.1159/000337460