과제정보
This study was supported by National Research Foundation of Korea (NRF-2021R1A2C1013606), and Rural Development Administration (Project No. PJ015001032021), Republic of Korea.
참고문헌
- Jansen R, van Embden JDA, Gaastra W, Schouls LM. 2002. Identification of genes that are associated with DNA repeats in prokaryotes. Mol. Microbiol. 43: 1565-1575. https://doi.org/10.1046/j.1365-2958.2002.02839.x
- Horvath P, Barrangou R. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science 327: 167-170. https://doi.org/10.1126/science.1179555
- Lee HJ, Lee SJ. 2021. Advances in accurate microbial genome-editing CRISPR technologies. J. Microbiol. Biotechnol. 31: 903-911. https://doi.org/10.4014/jmb.2106.06056
- Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, et al. 2014. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 343: 1247997. https://doi.org/10.1126/science.1247997
- Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337: 816-821. https://doi.org/10.1126/science.1225829
- Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol. 31: 233-239. https://doi.org/10.1038/nbt.2508
- Cong L, Ran FA, Cox D, Lin SL, Barretto R, Habib N, et al. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science 339: 819-823. https://doi.org/10.1126/science.1231143
- Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, et al. 2018. Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science 361: 1259-1262. https://doi.org/10.1126/science.aas9129
- Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA. 2013. Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 41: 7429-7437. https://doi.org/10.1093/nar/gkt520
- Larson MH, Gilbert LA, Wang X, Lim WA, Weissman JS, Qi LS. 2013. CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nat. Protoc. 8: 2180-2196. https://doi.org/10.1038/nprot.2013.132
- Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, et al. 2013. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell 152: 1173-1183. https://doi.org/10.1016/j.cell.2013.02.022
- Fu YF, Sander JD, Reyon D, Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat. Biotechnol. 32: 279-284. https://doi.org/10.1038/nbt.2808
- Lee HJ, Kim HJ, Lee SJ. 2021. Mismatch intolerance of 5'-truncated sgRNAs in CRISPR/Cas9 enables efficient microbial single-base genome editing. Int. J. Mol. Sci. 22: 6457. https://doi.org/10.3390/ijms22126457
- Moghadam F, LeGraw R, Velazquez JJ, Yeo NC, Xu C, Park J, et al. 2020. Synthetic immunomodulation with a CRISPR super-repressor in vivo. Nat. Cell Biol. 22: 1143-1154. https://doi.org/10.1038/s41556-020-0563-3
- Kim B, Kim HJ, Lee SJ. 2020. Effective blocking of microbial transcriptional initiation by dCas9-NG-mediated CRISPR interference. J. Microbiol. Biotechnol. 30: 1919-1926. https://doi.org/10.4014/jmb.2008.08058
- Lee HJ, Kim HJ, Lee SJ. 2020. CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs. Genome Res. 30: 768-775. https://doi.org/10.1101/gr.257493.119
- Kim B, Kim HJ, Lee SJ. 2020. Regulation of microbial metabolic rates using CRISPR interference with expanded PAM sequences. Front. Microbiol. 11: 282. https://doi.org/10.3389/fmicb.2020.00282
- Khakimzhan A, Garenne D, Tickman B, Fontana J, Carothers J, Noireaux V. 2021. Complex dependence of CRISPR-Cas9 binding strength on guide RNA spacer lengths. Phys. Biol. 18: 056003. https://doi.org/10.1088/1478-3975/ac091e