Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Impact of Environmental Factors on Phosphorus Removal of Bacillus licheniformis Isolated from Domestic Sewage

생활하수에서 분리된 Bacillus licheniformis의 인 제거에 대한 환경적인 인자의 영향

  • Han, Seok-Soon (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Park, Sang-Wook (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kim, Deok-Won (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Park, Ji-Su (Field Quality Control Gimcheon part, Doosan Corporation Electro-Materials) ;
  • Oh, Eun-Ji (Water and Land Research Group/Division for Natural Environment, Korea Environment Institute) ;
  • Yoo, Jin (Indoor Environment Division, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Kim, Deok-Hyeon (National Institute of Environmental Research) ;
  • Chung, Keun-Yook (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • 한석순 (충북대학교 환경생명화학과) ;
  • 박상욱 (충북대학교 환경생명화학과) ;
  • 김덕원 (충북대학교 환경생명화학과) ;
  • 박지수 (두산전자 Field Quality Control 김천 part) ;
  • 오은지 (한국환경정책평가연구원 자연환경연구실) ;
  • 유진 (인천광역시보건환경연구원 생활환경과) ;
  • 김덕현 (국립환경과학원 토양지하수과) ;
  • 정근욱 (충북대학교 환경생명화학과)
  • Received : 2020.12.15
  • Accepted : 2021.01.26
  • Published : 2021.02.28
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Abstract

This study was initiated to isolate the microorganisms removing phosphorus (P) from domestic sewage and to investigate the effects of environmental factors on the growth and P removal of the isolated bacteria. Microorganisms isolated from the sewage were identified as Chryseobacterium sp., Stenotrophomonas maltophilia, and Bacillus licheniformis. Among them, Bacillus licheniformis was selected as the P removal microorganism. The environmental factors considered in this study included initial phosphorus concentration, temperature, pH, and carbon source. At initial P concentrations of 10, 20, and 30 mg/L, the P removal efficiencies were 100.0%, 84.0%, and 16.5%, respectively. At 20℃, 30℃, and 40℃, the P removal efficiencies were 0%, 75.8%, and 60.6%, respectively. The removal efficiencies of phosphorus according to pH were 1.6%, 91.7%, and 51.1% at pH 5, pH 7, and pH 9, respectively. Using glucose, acetate, and glucose + acetate as carbon sources yielded P removal efficiencies of 80.9%, 33.6%, and 54.1%, respectively. Therefore, the results from the study demonstrated that the P removal efficiencies of Bacillus licheniformis were the highest when the initial P concentration, temperature, pH, and carbon source were 10 mg/L, 30℃, 7, and glucose, respectively.

Keywords

Acknowledgement

본 연구는 한국연구재단의 이공분야기초연구사업 연구비지원 (과제번호 2019R1F1A106325212)에 의해 수행되었습니다.

References

  1. Barnard, J. L., 1976, A Review of phosphorus removal in the activated sludge process, Water SA., 2, 136-144.
  2. Brannen, P. M., Kenney, D. S., 1997, Kodiak-a successful biologocal control product for suppression of soil-borne plant pathogens of cotton, J. Indust. Microbiol. Biotechnol., 19, 169-171. https://doi.org/10.1038/sj/jim/2900439
  3. Brdjanovic, D., Hooijmans, C. M., van Loosdrecht, M. C. M., Alaerts, G. J., Heijnen, J. J., 1996, The dynamic effects of potassium limitation on biological phosphorus removal, Water Res., 30, 303-313.
  4. Cech, J. S., Hartman, P., 1993, Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal systems, Water Research., 27, 1219-1225. https://doi.org/10.1016/0043-1354(93)90014-9
  5. Cho, W. S., Yoon, S. J., La, C. S., 2003, Recovery of N and Presources from animal wastewater by struvite crystallization, Journal of Animal Science and Technology, 45, 875-884. https://doi.org/10.5187/JAST.2003.45.5.875
  6. Choi, W. J., Park, K. M., Yoon, B. G., Kim, M. C., Oh, K. J., 2009, A Study on the recovery of phosphorus resources in sewage sludge using Struvite crystallization, Journal of Korean Society of Environmental Engineers, 31, 557-564.
  7. Converti, A., Rovatti, C., Borgh, D., 1995, Biological removal of phosphorus from wastewaters by alternating aerobic and anaerobic conditions, Water Res., 27, 791-798. https://doi.org/10.1016/0043-1354(93)90142-5
  8. de Graaff, D. R., van Loosdrecht, M. C., Pronk, M., 2020, Biological phosphorus removal in seawater-adapted aerobic granular sludge, Water Research, 172, 115531. https://doi.org/10.1016/j.watres.2020.115531
  9. Florentz, M., Caille, D., Bourdon, F. Sibony, J., 1987, Biological phosphorus removal in France, Water Sci. Technol., 19, 1171-1173. https://doi.org/10.2166/wst.1987.0003
  10. Gebremariam, S. Y., Beutel, M. W., Christian, D., Hess, T. F., 2012, Effects of glucose on the performance of enhanced biological phosphorus removal activated sludge enriched with acetate, Bioresource Technology, 121, 19-24. https://doi.org/10.1016/j.biortech.2012.06.086
  11. Grady, C. P. L., Daigger, G. T., Lim, H. C., 1999, Biological wastewater treatment, 2nd Ed. Marcel Dekker, New York.
  12. Hornbaek, T., Jakobsen, M., Dynesen, J., Nielsen, A. K., 2004, Global transcription profiles and intracellular pH regulation measured in Bacillus licheniformis upon external pH upshifts, Archives of microbiology, 182, 467-474. https://doi.org/10.1007/s00203-004-0729-6
  13. Kargi, F., Uygur, A., 2002, Nutrient removal performance of a sequencing batch reactor as a function of the sludge age, Enzyme Microb. Technol., 31, 842-847. https://doi.org/10.1016/S0141-0229(02)00209-0
  14. Khani, M., Bahrami, A., Chegeni, A., Ghafari, M. D., Zadeh, A. M., 2016, Optimization of carbon and nitrogen sources for extracellular polymeric substances production by Chryseobacterium indologenes MUT. 2., Iranian Journal of Biotechnology, 14, 13. https://doi.org/10.15171/ijb.1266
  15. Kim, J. H., Jung, J. T., Kim, H. K., 2006, Removal of nitrogen and phosphorus in anaerobic fermentation supernatant by struvite crystallization, Korean Geo-Environmental Society, 7, 5-12.
  16. Krishnaswamy, U., Muthusamy, M., Perumalsamy, L., 2009, Studies on the efficiency of the removal of phosphate using bacterial consortium for the biotreatment of phosphate wastewater, Eur. J. Appl. Sci., 1, 6-15.
  17. Krzywonos, M., Cibis, E., Miskiewicz, T., Kent, C. A., 2008, Effect of temperature on the efficiency of the thermo-and mesophilic aerobic batch biodegradation of high-strength distillery wastewater (potato stillage), Bioresource Technology, 99, 7816-7824. https://doi.org/10.1016/j.biortech.2008.01.063
  18. Levin, G. V., Sharpiro, J., 1965, Metabolic uptake of phosphorus by wastewater organics, J. Wat. Pollut. ControlFed., 37, 800.
  19. McClintock, S. A., Randall, C. W., Pattarkine, V. M., 1993, Effect of temperature and mean cell residence time on biological nutrient removal processes, Water Environ. Res., 65, 110-118. https://doi.org/10.2175/WER.65.2.3
  20. Mino, T., van Loosdrecht, M. C., Heijnen, J. J., 1998, Microbiology and biochemistry of the enhanced biological phosphorus removal process, Wat. Res., 32, 3193-3207. https://doi.org/10.1016/S0043-1354(98)00129-8
  21. Murthy, S. N., Novak, J. T., 1998, Effects of potassium Ion on sludge settling, Dewatering and effluent properties, Water Sci. Technol., 37, 317-324. https://doi.org/10.2166/wst.1998.0655
  22. Norbert, J., P pel, H. J., 1994, Phosphate release of sludge from enhanced biological P-removal during digestion, Proceedings of IAWQ 17th Biennial International Conference, 1, 167-174.
  23. Panswad, T., Doungchai, A., Anotai, J., 2003, Temperature effect on microbial community of enhanced biological phosphorus removal system, Water Res., .37, 409-415. https://doi.org/10.1016/S0043-1354(02)00286-5
  24. Richard, I. S., 1989, Principles and practice of phosphorus and nitrogen removal from municipal wastewater, The Soap & Detergent Association, New York, 123-139.
  25. Ryznar-Luty, A., Krzywonos, M., Cibis, E., Miskiewicz, T., 2008, Aerobic biodegradation of vinasse by a mixed culture of bacteria of the genus bacillus: Optimization of temperature, pH and oxygenation state, Polish Journal of Environmental Studies, 17, 101-112.
  26. Sedlak, R. I., 1991, Phosphorus and nitrogen removal from municipal wastewater, 2nd Ed., The soap and detergent association, Lewis Publishers, New York, 141-163.
  27. Song, M. Y., Jeon, M. S., Lee, H. D., Jeong, B. S., 2015, A Study on the traveling route and control method of eutrophication sources in Han River basin, Gyeonggi Research Institute, Korea, 1-111.
  28. Stephens, H. L., Stensel, H. D., 1998, Effect of operating conditions on biological phosphrous removal, Water Envir. Res., 70, 360-369.
  29. Tchobanoglus, G., Burton, F. L., Stensel, H. D., 2003, Waste Engineering, Mcgraw-Hill Inc., New York.
  30. Tracy, K. D., Flammino, A., 1985, Kinetics of biological phosphorous removal, Presented at the 58th annual waste pollution control federation conference, Kansas City, Missouri, 12, 102.
  31. Wiegert, T., Homuth, G., Versteeg, S., Schumann, W., 2001, Alkaline shock induces the bacillus subtilisσW regulon, Molecular microbiology, 41, 59-71. https://doi.org/10.1046/j.1365-2958.2001.02489.x
  32. Won, S. Y., Park, S. G., Lee, S. I., 2000, Removal of nitrogen and phosphorus using struvite crystallization, Journal of Korean Society of Environmental Engineers, 22, 599-607.
  33. Xiao, C. Q., Chi, R. A., He, H., Zhang, W. X., 2009, Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines, Journal of Central South University of Technology, 16, 581-587. https://doi.org/10.1007/s11771-009-0097-0
  34. Yeo, S. M., Lee, Y. O., 2006, Changes of the bacterial community structure depending on carbon source in biological phosphate removing process, Korean Society Of Environmental Engineers, 28, 165-172.
  35. Zafiri, C., Kornaros, M., Lyberatos, G., 1999, Kinetic modeling of biological phosphorus removal with a pure culture of Acinetobacter sp. under aerobic, anaerobic, and transient operating conditions, Water Res., 33, 2769-2788. https://doi.org/10.1016/S0043-1354(98)00522-3