International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Antioxidant capacity of silkworm pupa according to extraction condition, variety, pupation time, and sex

  • Lee, Ji Hae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jo, You-Young (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Seong-Wan (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kweon, HaeYong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2021.08.18
  • Accepted : 2021.11.24
  • Published : 2021.12.31
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Abstract

Silkworm pupa has been used as an edible insect with the high quality of protein and unsaturated fatty acids. In this study, antioxidant activities of pupa according to variety, pupation day, sex, and extraction solvent were analyzed. The 30% ethanol extract showed highest radical scavenging activity compared with the DW, hexane, and 70-100% ethanol extracts. In the DPPH and ABTS radical scavenging assay according to the type of pupa, the antioxidant effect was increased in female with the early stage of pupation day. In cell-based assay, reactive oxygen species (ROS) level was decreased in pupa groups by -30 to -50% followed by tert-butyl hydroperoxide (t-BHP) treatment. The ROS levels were significantly reduced in 7th day in each variety. In conclusion, the free radical and ROS scavenging effects were increased in female pupa with the early pupation day. The result could be used for development of bioactive materials using silkworm pupa.

Keywords

Acknowledgement

This study was supported by the 'Research Program for Agricultural Science & Technology Development' (Grant No. PJ01502202), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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