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Effect of extraction conditions on radical scavenging and cholesterol metabolism regulating capacity of silkworm larvae

  • Kim, Soo Hyun (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jo, You-Young (Research Policy Bureau, Rural Development Administration) ;
  • Kweon, HaeYong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Ji Hae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2021.08.31
  • Accepted : 2021.10.01
  • Published : 2021.12.31

Abstract

High blood cholesterol levels and oxidized cholesterol are risk factors for cardiovascular disease, which displays high annual incidence. Although studies on sericulture products, including pupae, silk protein, and blood lymph, as hypocholesterolemic substances have been reported, insufficient research in this field has been focused on silkworm larvae. Six larval extracts (Low temperature distilled water, LW; hot temperature distilled water, HW; and 30-100% ethanol, E30-E100) were prepared, and their effects on cholesterol metabolism were examined. LW most potently reduced the risk of cholesterol-related disorders. Polyphenols were highly represented in LW, corresponding with its increased antioxidant potency. The cholesterol biosynthesis enzyme, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) was strongly inhibited by LW. Hepatocytes over-expressed LDL receptor (LDLR) after LW stimulation, promoting cholesterol elimination from plasma. LW also increased ATP binding cassette transporter 1 (ABCA1) gene expression, upregulating HDL biogenesis. In conclusion, LW exhibited strong antioxidant activity, suppressed cholesterol biosynthesis, improved LDL uptake from plasma, and upregulated HDL biosynthesis. In aggregate, these activities could reduce blood cholesterol levels and prevent cardiovascular disease.

Keywords

Acknowledgement

This study was supported by the 'Research Program for Agricultural Science & Technology Development' (Grant No. PJ01510702), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

References

  1. Ademosun AO, Oboh G, Passamonti S, Tramer F, Ziberna L., Boligon AA, et al. (2015) Phenolics from grapefruit peels inhibit HMG-CoA reductase and angiotensin-I converting enzyme and show antioxidative properties in endothelial EA. Hy 926 cells. Food Sci Hum Wellness 4, 80-85. https://doi.org/10.1016/j.fshw.2015.05.002
  2. Bae SM, Jo YY, Lee KG, Kim HB, Kweon H (2016) Antioxidant activity of silkworm powder treated with protease. Int J Indust Entomol 33, 78-84. https://doi.org/10.7852/IJIE.2016.33.2.78
  3. Cho SH, Park YY, Yoon JY, Choi SW, Ha TY (2006) The effect of polyphenols from safflower seed on HMG-CoA reductase (HMGR) activity, LDL oxidation and apo A1 secretion. Korean J Food Sci Technol 38, 279-283.
  4. Dayton S, Chapman JM, Pearce ML, Popjak GJ (1970) Cholesterol, atherosclerosis, ischemic heart disease, and stroke. Ann Intern Med 72, 97-109. https://doi.org/10.7326/0003-4819-72-1-97
  5. Drotningsvik A, Vikoren LA, Mjos SA, Oterhals A, Pampanin D, Flesland O, et al. (2018) Water-soluble fish protein intake led to lower serum and liver cholesterol concentrations in obese zucker fa/fa rats. Mar Drugs 16, 149. https://doi.org/10.3390/md16050149
  6. Gil-Ramirez A, Caz V, Smiderle FR, Martin-Hernandez R, Largo C, Tabernero M, et al. (2016) Water-soluble compounds from Lentinula edodes influencing the HMG-CoA reductase activity and the expression of genes involved in the cholesterol metabolism. J Agric Food Chem 64, 1910-1920. https://doi.org/10.1021/acs.jafc.5b05571
  7. Hassanpour S, MaheriSis N, Eshratkhah B (2011) Plants and secondary metabolites (Tannins): A Review. Int J For Soil Eros 1, 47-53.
  8. Hu J, Zhang Z, Shen WJ, Azhar S (2010) Cellular cholesterol delivery, intracellular processing and utilization for biosynthesis of steroid hormones. Nutr Metab 7, 1-25. https://doi.org/10.1186/1743-7075-7-1
  9. Jo YY, Kim SB, Eom TD, Park SW, Kim SR, Kim SW, et al. (2019) Sterilization effect and fatty acid composition of silkworm powder (Bombyx mori L.) by heat treatment. Int J Indust Entomol 39, 39-44. https://doi.org/10.7852/ijie.2019.39.1.39
  10. Jo YY, Kim S, Lee JH, Kweon H, Ju WT, Kim HB, et al. (2020) Characteristics of the 3 rd day of 5 th instar silkworm powder: effect of preparation method. Int J Indust Entomol 40, 22-27. https://doi.org/10.7852/ijie.2020.40.1.22
  11. Ju WT, Kim HB, Kim KY, Sung GB, Kim YS (2015a) Screening of 1-deoxynojirimycin (DNJ) producing bacteria using mulberry leaf. Int J Indust Entomol, 31, 48-55. https://doi.org/10.7852/IJIE.2015.31.2.48
  12. Ju WT, Kim KY, Sung GB, Kim YS (2015b) Quantitative analysis of rutin content using silkworm genetic resources. Int J Indust Entomol 31, 56-61. https://doi.org/10.7852/IJIE.2015.31.2.56
  13. Kim HB, Kim JB, Ju WT, Kim SL, Lim JD (2017) Development of powder with increased rutin content from mulberry leaves for the application of food materials. Int J Indust Entomol 35, 77-82. https://doi.org/10.7852/IJIE.2017.35.2.77
  14. Lee JH, Lee SY, Kim B, Seo WD, Jia Y, Wu C, et al. (2015) Barley sprout extract containing policosanols and polyphenols regulate AMPK, SREBP2 and ACAT2 activity and cholesterol and glucose metabolism in vitro and in vivo. Food Res Int 72, 174-183. https://doi.org/10.1016/j.foodres.2015.03.041
  15. Lee S, Koo B, Ju WT, Kim HB, Kweon H, Lee JH (2021) Effect of extraction conditions on chemical composition and antioxidant properties of mulberry fruit. Int J Indust Entomol, 42, 25-32. https://doi.org/10.7852/IJIE.2021.42.2.25
  16. Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4, 118. https://doi.org/10.4103/0973-7847.70902
  17. Luo Y, Wang L, Lv Y, Wu X, Hou C, Li J (2020) Regulation mechanism of silkworm pupa oil PUFAs on cholesterol metabolism in hepatic cell L-02. J Sci Food Agric 100, 1418-1425. https://doi.org/10.1002/jsfa.10115
  18. Madison BB (2016) Srebp2: A master regulator of sterol and fatty acid synthesis1. J Lipid Res 57, 333-335. https://doi.org/10.1194/jlr.C066712
  19. Marques LR, Diniz TA, Antunes BM, Rossi FE, Caperuto EC, Lira FS, et al. (2018) Reverse cholesterol transport: molecular mechanisms and the non-medical approach to enhance HDL cholesterol. Front Physiol 9, 526. https://doi.org/10.3389/fphys.2018.00526
  20. Nam YR, Ko YE, Ly SY (2018) Anti-obesity and LDL-cholesterol lowering effects of silkworm hemolymph in C57BL/6N mice fed high fat diet. J Nutr Health 51, 201-207. https://doi.org/10.4163/jnh.2018.51.3.201
  21. Nino MC, Reddivari L, Osorio C, Kaplan I, Liceaga AM (2021) Insects as a source of phenolic compounds and potential health benefits. J Insect Food Feed 1-12.
  22. Park YM, Kim KA, Kang MU, Park KH, Nho SK (2014) Screening of silkworm strains for efficient recombinant protein production by Autographa californica nucleopolyhedrosis virus (AcNPV). Int J Indust Entomol 28, 10-18. https://doi.org/10.7852/IJIE.2014.28.1.10
  23. Poljsak B, Dahmane R (2012) Free radicals and extrinsic skin aging. Dermatol Res pract 2012.
  24. Ryu KS, Lee HS, Kim KY, Kim MJ, Sung GB, Ji SD, et al. (2013) 1-Deoxynojirimycin content and blood glucose-lowering effect of silkworm (Bombyx mori) extract powder. Int J Indust Entomol 27, 237-242. https://doi.org/10.7852/IJIE.2013.27.2.237
  25. Shahidi F, Ambigaipalan P (2015) Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects-A review. J Funct Foods 18, 820-897. https://doi.org/10.1016/j.jff.2015.06.018
  26. Sharpe LJ, Brown AJ (2013) Controlling cholesterol synthesis beyond 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). J Biol Chem 288, 18707-18715. https://doi.org/10.1074/jbc.R113.479808
  27. Son KH, Lee JY, Lee JS, Kang SS, Sohn HY, Kwon CS (2017) Screening of phenolic compounds with inhibitory activities against HMG-CoA reductase. J Life Sci 27, 325-333. https://doi.org/10.5352/JLS.2017.27.3.325
  28. Valenzuela A, Sanhueza J, Nieto S (2003) Cholesterol oxidation: health hazard and the role of antioxidants in prevention. Biol Res 36, 291-302.
  29. Wang MX, Lu Y, Cai ZZ, Liang S, Niu YS, Miao YG (2013) Phenol oxidase is a necessary enzyme for the silkworm molting which is regulated by molting hormone. Mol Biol Rep 40, 3549-3555. https://doi.org/10.1007/s11033-012-2428-8