한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제34권1호
  • /
  • Pages.107-113
  • /
  • 2021
  • /
  • 1225-4339(pISSN)
  • /
  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

증숙 더덕 에탄올 추출물에 대한 항산화·항균 활성

Antioxidative and Antimicrobial Effects of Ethanol Extract of Codonopsis lanceolata by Steaming Times

  • 이희경 (농업회사법인(유)청솔식품 기업부설연구소) ;
  • 최온유 (농업회사법인(유)청솔식품 기업부설연구소) ;
  • 최두복 (조선대학교 신산업융합학부) ;
  • 최현숙 (충청대학교 식품영양외식학부)
  • Lee, Hee-Kyung (Research Institute, Cheongsol Food R & D, Agricultural Company Limited) ;
  • Choi, On-Yu (Research Institute, Cheongsol Food R & D, Agricultural Company Limited) ;
  • Choi, Du-Bok (Dept. of Advanced Industry Convergence, Chosun University) ;
  • Choi, Hyun-Suk (Dept. of Hotel Culinary Arts Patissier & Nutrition, Chungcheong University)
  • 투고 : 2021.01.19
  • 심사 : 2021.02.04
  • 발행 : 2021.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was investigated the contents of total polyphenol, flavonoids and antioxidant and antimicrobial activities of Codonopsis lanceolata extracts according to different steaming times. The contents of total polyphenol and flavonoid proportionally increased from 6.45 mgGAE/g to 18.26 mgGAE/g and 2.01 mgRE/g to 6.12 mgRE/g according to ethanol extracts at EDS7. DPPH radical scavenging activity was found to have been 15.26~65.2% and showed the highest level of antioxidant activity at EDS7 was 65.2%. The activity of ABTS radical scavenging and SOD-like activity were also the same result. DPPH and ABTS radical scavenging activity was related to the number of steaming, and the scavenging activity was increased up to 7 times of steaming. The antimicrobial activity of EDS7 had strong antioxidant activity. Antimicrobial activities were examined against 5 microorganisms related to pathogens and food poisoning. The antimicrobial Activity was different depending on the bacteria, but it was effective at the concentration of 300 mg/mL rather than 150 mg/mL. These results showed that Codonopsis lanceolata extracts with a different number of steaming would be conducted to confirm the possibility of developing antimicrobial and antioxidant. It will be helpful in the study of component analysis of Codonopsis lanceolata extracts processed products.

키워드

참고문헌

  1. Bice F, Andrea H. 2003. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) generation by silica in inflammation and fibrosis. Free Radical Biol Med 34:1507-1516 https://doi.org/10.1016/S0891-5849(03)00149-7
  2. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  3. Brannen AL. 1975. Toxicology and biochemistry of butylated hydoroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 52:59-63 https://doi.org/10.1007/BF02901825
  4. Byeon SE, Choi WS, Hong EK, Lee J, Rhee MH, Park HJ, Cho JY. 2009. Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses. Arch Pharmacal Res 32:813-822 https://doi.org/10.1007/s12272-009-1601-7
  5. Haarold JB. 1990. Microbiological Applications: A Laboratory Manual in General Microbiology. 5th ed. p.703. WCB Publishers
  6. Ham SS, Oh DH, Hong JK, Lee JH. 1997. Antimutagenic effects of juices from edible Korean wild herbs. J Food Sci Nutr 2:155-161
  7. Han EG, Sung IS, Moon HG, Cho SY. 1998. Effect of Codonopsis lanceolata water extract on the levels of lipid in rats fed high fat diet. J Korean Soc Food Sci Nutr 27:940-944
  8. Ho CT. 1992. Phenolic compounds in food: An overview. In Huang MT, Ho CT, Lee CY (Eds.), Phenolc Compounds in Food and Their Effects on Health II: Antioxidants and Cancer Prevention. pp.2-7. American Chemical Society
  9. Hong HD, Kim YC, Rho JH, Kim KT, Lee CY. 2007. Changes on physicochemical properties of Panax ginseng C. A. Meyer during repeated steaming process. J Ginseng Res 31:222-229 https://doi.org/10.5142/JGR.2007.31.4.222
  10. Hwang CR, Oh SH, Kim HY, Lee SH, Hwang IG, Shin YS, Lee JS, Jeong HS. 2011. Chemical composition and antioxidant activity of deoduk (Codonopsis lanceolata) and doragi (Platycodon grandiflorum) according to temperature. J Korean Soc Food Sci Nutr 40:798-803 https://doi.org/10.3746/JKFN.2011.40.6.798
  11. Ichikawa M, Ohta S, Komoto N, Ushijima M, Kodera Y, Hayama M, Shirota O, Sekita S, Kuroyanagi M. 2009. Simultaneous determination of seven saponins in the roots of Codonopsis lanceolata by liquid chromatography-mass spectrometry. J Nat Med 63:52-57 https://doi.org/10.1007/s11418-008-0294-4
  12. Jeon SM, Kim SY, Kim IH, Go JS, Kim HR, Jeong JY, Lee HY, Park DS. 2013. Antioxidant activities of processed deoduck (Codonopsis lanceolata) extract. J Korean Soc Food Sci Nutr 42:924-932 https://doi.org/10.3746/JKFN.2013.42.6.924
  13. Jeong HY. 1991. Aging free radical arteriosclerosis. Life Sci 1:2-14
  14. Jin TY, Quan WR, Wang MH. 2008. Changes of physicochemical and sensory characteristics in the Codonopsis lanceolata Saengsik, uncooked food by different drying methods. Korean J Food Sci Technol 40:721-725
  15. Jung LS, Yoon WB, Park SJ, Park DS, Ahn JH. 2012. Evaluation of physicochemical properties and biological activities of steamed and fermented Deodeok (Codonopsis lanceolata). Korean J Food Sci Technol 44:135-139 https://doi.org/10.9721/KJFST.2012.44.1.135
  16. Kang DH, Kim MY. 2015. Comparative phenolic composition and antioxidant properties of honey and honeycomb extracts. J Life Sci 25:1169-1175 https://doi.org/10.5352/JLS.2015.25.10.1169
  17. Kang MC, Lee JY, Ko RK, Kim HB, Hong SH, Kim GO. 2009. Melanin inhibitory effect and anti-inflammatory effects of Dictyota coriacea extracts derived from adjacent sea of the Jeju island. Korean J Biotechnol Bioeng 23:311-316
  18. Kang MK, Kim JS, Kim GC, Choi SY, Kim KM. 2015. Evaluation of physicochemical properties and enhancement of antioxidant activities of Dioscorea batatas by stepwise steaming process. J East Asian Soc Diet Life 25:1049-1057 https://doi.org/10.17495/easdl.2015.12.25.6.1049
  19. Kim HJ, Han CH, Kim NY, Lee EK, Lee KN, Cho HE, Choi YH, Chong MS. 2010c. Effect of garlic extracts with extraction conditions on antioxidant and anticancer. Korean J Orient Physiol Pathol 24:111-117
  20. Kim JG, Kang YM, Eum GS, Ko YM, Kim TY. 2003. Antioxidative activity and antimicrobial activity of extracts from medicinal plants (Akebia quinate Decaisn, Scirus fluviatilis A. Gray, Gardenia jasminoides for grandiflora Makino). J Agric Life Sci 37:69-75
  21. Kim NY, Chae HS, Lee IS, Kim DS, Seo KT, Park SJ. 2010b. Analysis of chemical composition and antioxidant activity of Codonopsis lanceolata skin. J Korean Soc Food Sci Nutr 39:1627-1633 https://doi.org/10.3746/JKFN.2010.39.11.1627
  22. Kim SS, Jeong MH, Seo YC, Kim JS, Kim NS, Woon WB, Ahn J, Hwang B, Park DS, Park SJ, Lee HY. 2010a. Comparison of antioxidant activity by high pressure extraction of Codonopsis lanceolata from different production areas. Korean J Med Crop Sci 18:248-254
  23. Kitani K, Minami C, Yamamoto T, Kanai S, Ivy GO, Carrillo MC. 2002. Pharmacological interventions in aging and ageassociated disorders: Potentials of propargylamines for human use. Ann N Y Acad Sci 959:295-307 https://doi.org/10.1111/j.1749-6632.2002.tb02101.x
  24. Klug D, Rabani J, Fridovich I. 1972. A direct demonstration of the catalytic action of superoxide dismutase through the use of pulse radiolysis. J Biol Chem 247:4839-4842 https://doi.org/10.1016/S0021-9258(19)44987-9
  25. Larson RA. 1988. The antioxidants of higher plants. Phytochemistry 27:969-978 https://doi.org/10.1016/0031-9422(88)80254-1
  26. Lee HG, Choi OY, Choi DB, Choi HS. 2020. Effect of Deodeok extract on the skin function improvement. Korean J Food Nutr 33:505-511 https://doi.org/10.9799/KSFAN.2020.33.5.505
  27. Lee JH. 2002. Immunostimulative effect of hot-water extract from Codonopsis lanceolata on lymphocyte and clonal macrophage. Korean J Food Sci Technol 34:732-736
  28. Lee OH, Lee HB, Son JY. 2004. Antimicrobial activities and nitrite-scavenging ability of olive leaf fractions. Korean J Soc Food Cookery Sci 20:204-221
  29. Link A, Balaguer F, Goel A. 2010. Cancer chemoprevention by dietary polyphenols: Promising role for epigenetics. Biochem Pharmacol 80:1771-1792 https://doi.org/10.1016/j.bcp.2010.06.036
  30. Maeng YS, Park HK. 1991. Antioxidant activity of ethanol extract from Dodok (Codonopsis lanceolata). Korean J Food Sci Technol 23:311-316
  31. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  32. Moon TC, Park JO, Chung KW, Son KH, Kim HP, Kang SS, Chang HW. 1999. Anti-inflammatory activity of the flavonoid components of Lonicera japonica. Yakhak Hoeji 43:117-123
  33. Oh JH, Kim EH, Kim JL, Moon YI, Kang YH, Kang JS. 2004. Study on antioxidant potency of green tea by DPPH method. J Korean Soc Food Sci Nutr 33:1079-1084 https://doi.org/10.3746/JKFN.2004.33.7.1079
  34. Park SJ, Seong DH, Park DS, Kim SS, Gou JY, Ahn JH, Yoon WB, Lee HY. 2009. Chemical compositions of fermented Codonopsis lanceolata. J Korean Soc Food Sci Nutr 38:396-400 https://doi.org/10.3746/JKFN.2009.38.3.396
  35. Perron NR, Brumaghim JL. 2009. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys 53:75-100 https://doi.org/10.1007/s12013-009-9043-x
  36. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  37. Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152-159 https://doi.org/10.1016/S1360-1385(97)01018-2
  38. Safer AM, al-Nughamish AJ. 1999. Hepatotoxicity induced by the antioxidant food additive, butylated hydroxytoluene (BHT), in rats: An electron microscopical study. Histol Histopathol 14:391-406
  39. Shim KS, Park GH, Choi CJ, Na CS. 2004. Decreased triglyceride and cholesterol levels in serum, liver and breast muscle in broiler by the supplementation of dietary Codonopsis lanceolata root. Asian Australas J Anim Sci 17:511-513 https://doi.org/10.5713/ajas.2004.511
  40. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol 299:152-178 https://doi.org/10.1016/S0076-6879(99)99017-1
  41. Song CH, Seo YC, Choi WY, Lee CG, Kim DU, Chung JY, Chung HC, Park DS, Ma CJ, Lee HY. 2012. Enhancement of antioxidative activity of Codonopsis lanceolata by stepwise steaming process. Korean J Med Crop Sci 20:238-244 https://doi.org/10.7783/KJMCS.2012.20.4.238
  42. Song HM, Seo MS, Kim HM, Ahn MS, Lee YT. 2002. Antioxidative activity of barley polyphenol extract (BPE) separated from pearling by-products. Korean J Food Sci Technol 34:889-892
  43. Taruna H, Parihar MS. 1998. Reactive oxygen species andoxi- dative DNA damage. Ind J Physiol Pharmacol 42:440-452
  44. Weon JB, Yun BR, Lee J, Eom MR, Ko HJ, Lee HY, Park DS, Chung HC, Chung JY, Ma CJ. 2014. Effect of steamed Codonopsis lanceolata on spatial learning and memory in mice. Korean J Pharmacogn 45:48-54
  45. Williams RJ, Spencer JPE, Rice-Evans C. 2004. Flavonoids: Antioxidants or signaling molecules? Free Radic Biol Med 36:838-849 https://doi.org/10.1016/j.freeradbiomed.2004.01.001
  46. Won BR, Song EH, Kang GH, Chang MW, Yoon YH. 2001. Inhibition effect of Lactobacillus heiviticus CU631 on urease and vacuolating toxin activity of Helicobacter pylori. J Anim Sci Technol 43:931-940
  47. Yoon SR, Lee MH, Park JH, Lee IS, Kwon JH, Lee GD. 2005. Changes in physicochemical compounds with heating treatment of ginseng. J Korean Soc Food Sci Nutr 34:1572-1578 https://doi.org/10.3746/JKFN.2005.34.10.1572