Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Comparison of sample preparation methods for quantification of febantel, an anthelmintic agent, and its metabolites in rockfish (Sebastes schlegeli) muscle using liquid chromatography-tandem mass spectrometry

LC-MS/MS를 이용한 조피볼락 근육에서 구충제 febantel 및 그 대사체들의 정량분석을 위한 시료 전처리 방법의 비교 분석

  • Lim, Jae-Woong (Department of Aqualife Medicine, Chonnam National University) ;
  • Kwon, Inyeong (Smart Aquaculture Research Center, Chonnam National University) ;
  • Kim, Taeho (Department of Marine Production Management, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Kang, So Young (Department of Aqualife Medicine, Chonnam National University)
  • 임재웅 (전남대학교 수산생명의학과) ;
  • 권인영 (전남대학교 스마트수산양식연구센터) ;
  • 김태호 (전남대학교 해양생산관리학과) ;
  • 김위식 (전남대학교 수산생명의학과) ;
  • 강소영 (전남대학교 수산생명의학과)
  • Received : 2021.11.24
  • Accepted : 2021.12.06
  • Published : 2021.12.31
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Abstract

This study presents the evaluation of sample extraction and purification procedure for the determination of residues of febantel and its metabolites, fenbendazole, oxfendazole and oxfendazole sulfone in rockfish (Sebastes schlegeli) muscle using liquid chromatography-tandem mass spectrometry. Residues of febantel and its metabolites in rockfish muscle were analyzed using each different sample preparation method from Korean Food Standards Codex (KFSC), Food Safety and Inspection Service (FSIS, USA), and the modified FSIS method using QuEChERS kit (FSIS-Q), respectively. Each method was compared for mean recoveries and repeatabilities. Since FSIS-Q showed higher repeatabilities (coefficient of variation, CV of 2.4%~10.9%) than those of FSIS method (CV of 4.6%~17.5%), recoveries from FSIS-Q were compared with those from KFSC method. FSIS-Q showed significantly higher recoveries of 83.1%~110.1% (P < 0.05) than those from KFSC method of 64.7%~107.4%. In addition, FSIS-Q showed a good linearity over the range of 2.5~200 ㎍/kg, and excellent sensitivities with limit of detection of 0.46~0.71 ㎍/kg and limit of quantification of 1.08~2.15 ㎍/kg. Although all the sample preparation methods turned out to be able to meet CODEX guideline for all the compounds, FSIS method and FSIS-Q validated in this study could be applied to screening and quantification for residues of febantel and its metabolites in rockfish muscle with efficient preparation procedures.

Keywords

Acknowledgement

이 논문은 2021년 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구임(스마트수산양식연구센터).

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