Advances in nano research

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Medicinal aspects of Murraya koenigii mediated silver nanoparticles

  • Mumtaz, Sumaira (Department of Chemistry, University of Agriculture, Agriculture University Road) ;
  • Nadeem, Raziya (Department of Chemistry, University of Agriculture, Agriculture University Road) ;
  • Sarfraz, Raja A. (Department of Chemistry, University of Agriculture, Agriculture University Road) ;
  • Shahid, Muhammad (Department of Biochemistry, University of Agriculture, Agriculture University Road)
  • Received : 2020.05.20
  • Accepted : 2021.10.15
  • Published : 2021.12.25
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Abstract

The present work aimed to explore green approach via aqueous leaves extract of Murraya koenigii (ALEMk) for the synthesis of silver nanoparticles (AgNPsMk) in single step. The synthesis process was visualized with a color change and monitored by employing UV/Visible spectroscopy and a clear peak attained at 420 nm confirming the synthesis of AgNPsMk. The possible functional groups present in the extract which participated in the synthesis of AgNPsMk were identified with the help of FTIR spectroscopy. Further characterization using TEM images revealed the spherical shape of AgNPsMk with average particle size of 20 nm displaying well dispersion throughout the solution. Pronounced antioxidant activities of AgNPsMk at increased concentrations observed which evidencing strong radical scavenging ability. Moreover, AgNPsMk exhibited strong antibacterial behavior when tested against bacterial strains of Escherichia coli and Bacillus subtilis. Moving ahead, in vitro cytotoxicity work revealed potent cell viability loss appearing in AU565 and HeLa cancer cell lines on exposure to AgNPsMk at increased concentration. Finally, in vivo assessment carried out inside male Wistar rats indicated non toxic effect on examined liver tissues besides biochemical analysis including bilirubin, alkaline phosphtase (ALP) and serum glutamate pyruvate transaminase (SGPT) which found within the normal range when compared with control. The prior research work profoundly apprises the potential of green synthesized AgNPsMk to play a significant role in biomedical applications and formulations.

Keywords

References

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