Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Natural Enemies of the Asian Gypsy Moth, Lymantria dispar asiatica (Lepidoptera: Erebidae) and the Genetic Variation Analysis of L. dispar Multiple Nucleopolyhedrovirus

국내 매미나방(나비목: 태극나방과) 천적 및 매미나방 핵다각체병바이러스의 유전적 다양성 조사

  • Hwang, Hwal-Su (College of Agriculture and Life sciences, Kyungpook National University) ;
  • Lee, Young Su (Gyeonggi Agricultural Research and Extension Services) ;
  • Lee, Hee A (Gyeonggi Agricultural Research and Extension Services) ;
  • Choi, Duck Soo (Jeonnam Agricultural Research and Extension Services) ;
  • Lee, Kyeong-Yeoll (College of Agriculture and Life sciences, Kyungpook National University)
  • 황활수 (경북대학교 농업생명과학대학) ;
  • 이영수 (경기도농업기술원) ;
  • 이희아 (경기도농업기술원) ;
  • 최덕수 (전남농업기술원 친환경농업연구소) ;
  • 이경열 (경북대학교 농업생명과학대학)
  • Received : 2021.10.12
  • Accepted : 2021.11.05
  • Published : 2021.12.01
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Abstract

Asian gypsy moth, Lymantria dispar asiatica (Lepidoptera: Erebidae), which is an indigenous pest in Korea, a large outbreak has been reported in Gyeonggi-do, Chungcheong-do, and northern Gyeongsangbuk-do from 2019 in forest and nearby downtown areas, causing emotional damage to forests and city dwellers. During the indoor culture of gypsy moth eggs collected in Yecheon, Gyeongbuk in 2021 we found that 79.65% (321/403 of first instar larvae) were died due to Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) infection. Sequence analyses of 36 gypsy moths collected from 12 regions in Korea using LdMNPV late expression factor-8 (lef-8) and polyhedrin (polh) genes showed a genetic variation of 0.80% and 0.86%, respectively. Comparison to GenBank data showed that the Korean samples were most similar to LdMNPV in Japan, whereas most different to those of Turkey. These results showed a high infection rate of LdMNPV in Korea and LdMNPV is one of the important population regulators of the gypsy moth.

아시아계통의 매미나방(Lymantria dispar asiatica) (나비목: 태극나방과)은 국내 토착해충으로서 지역에 따라 돌발적으로 대발생한 사례가 있으며, 다양한 수목 및 농작물에 피해를 끼치는 광식성 해충이다. 특히 2019년 이후로 경기도, 충청도, 경북 북부지역에서 대발생하여 산림 및 인근지역 도심에 발생하여 산림 및 도시민들의 정서적 피해를 끼치기도 했다. 본 연구에서는 2020-2021년 경북 예천지역에서 알집을 채집하여 사육한 결과, 매미나방 핵다각체병바이러스(LdMNPV) 감염에 의해 79.65% (321/403마리)는 사육중 사망하였다. 염기서열 분석은 2021년 국내 12 지역에서 매미나방 유충을 36마리를 조사한 결과, LdMNPV의 late expression factor-8 (lef-8), polyhedrin (polh) 유전자의 종내변이율이 0.80%, 0.86%로 확인됐다. NCBI database 자료와 비교 분석한 결과 일본의 LdMNPV와 가장 유사했으며, 터키의 LdMNPV와 가장 큰 차이를 나타냈다. 본 조사를 통하여 LdMNPV는 높은 감염율을 나타냈고 매미나방 중요한 개체군 조절인자중 한가지로 작용할 것으로 판단된다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 공동연구사업(PJ015670)의 지원으로 수행되었습니다.

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