CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
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Development of Standard Operating Procedures (SOPs), Standardization, TLC and HPTLC Fingerprinting of a Polyherbal Unani Formulation

  • Naaz, Arjumand (P.G Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders) ;
  • Viquar, Uzma (Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders) ;
  • Naikodi, Mohammad Abdul Rasheed (Drug Standardization Research Unit, National Research Institute of Unani Medicine for Skin Disorders) ;
  • Siddiqui, Javed Inam (Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders) ;
  • Zakir, Mohammad (Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders) ;
  • Kazmi, Munawwar Husain (Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders) ;
  • Minhajuddin, Ahmed (National Research Institute of Unani Medicine for Skin Disorders)
  • Received : 2021.11.04
  • Accepted : 2021.11.24
  • Published : 2021.11.30
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Abstract

Background: Unani System of Medicine (USM) has its origin to Greece. To ensure and develop the quality, authenticity of Unani drugs, standardization on modern analytical parameter is essential requirement for drugs. Objectives: The aimed of the present study was to develop a standard profile of "Qurṣ-e-Mafasil" by systematic study through authenticated ingredients, pharmacognostic identification followed by physicochemical, TLC, HPTLC fingerprinting analysis as per standard protocol. Material and Methods: In this study three batches of "Qurṣ-e-Mafasil" QM were prepared by standard method as per UPI had been followed by organoleptic properties of formulation such as appearance, color, odor, taste. Powder Microscopy and physicochemical studies were carried out such as Uniformity of weight, Friability, Disintegration time, hardness, LOD, ash vales and extractive values in like aqueous, alcohol & hexane. Further qualitative tests such as Thin-Layer Chromatography (TLC), and High-Performance Thin Layer Chromatography (HPTLC) studies were also carried out to develop fingerprint pattern of the alcoholic solvent extract of QM. Phytochemical screening was carried out in different solvent extracts such as alcoholic, aqueous and chloroform extracts to detect the presence phytoconstituents in the formulation QM. Heavy metals, Microbial Load Contamination and pesticidal residues were also determined. Results: Qurṣ-e-Mafasil showed tablet-like appearance, light brown colour, mild pungent odour and acrid taste. Uniformity of weight (mg), friability (rpm), and hardness (kg/cm) and disintegration time was ranged between (500 to 503), (0.0340 to 0.038), (8.40 to 8.67) and (4-5 minutes) respectively for the three batches. Loss in weight on drying at 105℃ was ranged between (8.3425 to 8.7346). Extracted values were calculated in distilled water ranged between (30.9091 to 31.4358), hexane (1.1419 to 1.4281), and alcohol (3.3352 to 3.3962). The ash values recorded were ranged between (3.7336 to 3.8378), and acid insoluble ash (0.5859 to 0.6112).

Keywords

Acknowledgement

The author would like to record their gratitude to Director General, Central Council for research in Unani Medicine (CCRUM), New Delhi, India, for providing an excellent research environment to carry out the work.

References

  1. Ali HSS. Unani Advia Murrakaba, (New Delhi: Aejaz Publishing House), 2010, pp. 10.
  2. Anonymous. National Formulary of Unani Medicine, Part-II, Vol-I, 1st Ed. (New Delhi: Ministry of Health and Family welfare, Govt. of India, Dept. of AYUSH), 2001, pp. 17.
  3. Anonymous. WHO, Guidelines for assessing quality of herbal medicine with reference to contamination and residue, (India: WHO), 2007.
  4. Anonymous. Unani Medicine Kit developed by Central Council for Research in Unani Medicine, (New Delhi: Ministry of Health and Family Welfare, Govt. of India), pp.11.
  5. Anonymous. The Unani Pharmacopoeia of India, part-II, Vol-I, Ed.-I, (New Delhi: Ministry of Health & family Welfare, Dept Anonymous. of AYUSH), 2009, pp.142, 145-147, 150, 164-184, 195, 196, 278.
  6. Anonymous. The Unani Pharmacopoeia of India, part-II, Vol-II, Ed.-I, (New Delhi: Ministry of Health & family Welfare, Dept Anonymous. of AYUSH), 2010, pp. 278-280.
  7. Anonymous. Standard Unani Medical Terminology. Introduction (xv), Central Council for Research in Unani Medicine, (New Delhi: Ministry of AYUSH, Govt. of India), 2012, pp. 123, 290
  8. Anonymous. Unani Medicine Kit developed by Central Council for Research in Unani Medicine, (New Delhi: Ministry of Health and Family Welfare, Govt. of India), YNM, pp.11.
  9. Anonymous, The Unani Pharmacopoeia of India, Part-II, Vol-III, (New Delhi: Ministry of Health and family welfare, Govt. of India, Dept. of AYUSH), 2016, pp. 55-6.
  10. Anonymous. UNANI SYSTEM OF MEDICINE (The Science of Health and Healing); (New Delhi: Ministry of AYUSH, Govt. of India), 2016, pp. 1-14.
  11. Alam A, Siddiqui JI, Kazmi MH. Standardization of Unani Drugs on Modern Analytical Parameter: A Necessary step, JDDT, 2019; 9(4-s): 648-652.
  12. Bodeker G. and Burford G. Traditional, Complementary and Alternative Medicine Policy and Public Health Perspectives, (London: Imperial College Press), 2007.
  13. Hakeem Zillur Rahman, Jadeed Unani Dawasazi, Idara Kitabul-Shifa, Kucha Chelan, Darya Ganj, New delhi; June 2002, p.29.
  14. Iyengar MA. Pharmacognosy of Powder Drugs, (Manipal: Manipal Power Press), 1980, pp. 9-43.
  15. Kabeeruddin HM. Al-Qarabadeen, (New Delhi: Central Council for Research in Unani Medicine), 2006, pp.184, 186, 528, 1119, 1212.
  16. Khan MA, Ramooz-I Aazam Bazabanfarsi, vol.2, (New Delhi: Centeral Council for Research in Unani Medicine. Ministry of AYUSH, Govt. of India), 2006, pp. 283, 284,286.
  17. Kokate CK, Purohit AP, Gokhale SB, Pharmacognosy, (Pune: Nirali Parkashan), 2012, pp.107-110.
  18. Lachman L, Lieberman HA, Kanig JL. The Theory and Practice of Industrial Pharmacy. 3rd ed. (Mumbai: Varghese Publishing House), 1987, pp. 88, 297-301.
  19. Madhav NVS, Upadhyaya K, Bisht A. Phytochemical screening and standardization of polyherbal formulation for dyslipidemia, Int j pharm sci, 2011;3: 235-238.
  20. Tabri ASR. Firdous-ul-Hikmat, (New Delhi: Idara-e-kitab-us-shifa), 2010, pp. 291-293.
  21. William C Evans. Technique in microscopy, Pharmacognosy, 15th Ed., (Netherlands: Elsevier), 1983, pp. 538-547.
  22. WHO, Quality control methods for herbal material, (Geneva: World Health Organization), 2011.