• Title/Summary/Keyword: TLC

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Simple and Quantitative Analysis Method for Lactic Acid by TLC (젖산의 빠른 정량적 분석을 위한 TLC 최적 조건)

  • 최미화;조갑수;강희경;윤종선;서은성;류화원;장세효;윤승헌;김도만
    • KSBB Journal
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    • v.18 no.1
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    • pp.70-73
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    • 2003
  • TLC condition was developed for its simple separation and quantitative analysis of lactic acid. Rapid and clear separation of lactic acid by silica gel TLC plate was obtained by using nitromethane : 1-propanol : $H_2O$ (2 : 5 : 1.5, v/v/v) and a suitable dipping solution of 40 mg bromocresol purple in 100 mL 5% ethanol (pH 10.0). The lactic acid was shown as a bright yellow spot on a light cinnabar background. The quantitatively detectable concentration range of lactic acid was between 0.5 and 4% with 99.4%, confidence. Quantitative TLC analysis result was confirmed with HPLC and with enzymatic Quantitative analysis methods (by using lactate dehydrogenase).

Comparative Studies on the Assay Methods of Stevia Sweeteners (스테비아 감미성분의 정량법에 관한비교)

  • Kim, Nam-Soo;Oh, Sang-Lyong;Nam, Young-Jung;Min, Byong-Yong;Suh, Kee-Bong
    • Korean Journal of Food Science and Technology
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    • v.15 no.3
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    • pp.209-214
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    • 1983
  • Analytical methods on Stevia sweeteners are compared for their reproducibilities and recoveries. It is possible to separate stevioside, rebaudioside A, rebaudioside C, and dulcoside A through HPLC analysis. Steviolbioside, in addition to above 4 Stevia sweeteners, is detected through TLC scanner and TLC-FID assays. C.V.s on stevioside and rebaudioside A in HPLC analysis are 1.39 and 4.89%, which shows outstanding reproducibilities of this method. The recoveries of stevioside in HPLC, TLC scanner, and TLC-FID analyses are 97.7 89.4, and 97.3%. The recoveries of rebaudioside A in HPLC, TLC scanner, and TLC-FID assays are 90.8, 90.1, and 75.8%. Total content of Stevia sweeteners in 8 strains tested, ranges from 5 to 17% as dry weight basis.

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Improvement in Thin-layer Chromatography in a Quantitative Assay of Glycerol in Biodiesel (개선된 thin-layer chromatography를 이용한 바이오디젤 중의 글리세롤 정량분석)

  • Lee, Sang-Eun;Choi, Woo-Seok;Kang, Do-Hyung;Lee, Hyeon-Yong;Jung, Kyung-Hwan
    • Journal of Life Science
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    • v.23 no.4
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    • pp.537-541
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    • 2013
  • We analyzed glycerol using thin-layer chromatography (TLC) and compared the separation resolution of some mobile phases. When acetonitrile:distilled water (85:15 v/v) was used as a mobile phase, the band of glycerol on the TLC was more distinctly and rapidly separated. Using TLC analysis, we prepared a calibration curve for the glycerol concentration vs. the area of the glycerol band in which the glycerol concentration of the x-axis was converted into a log-scale ranging from 3.0 to 0.0625 (%, w/v). Based on this calibration curve, the residual glycerol concentration (0.2 [%, w/v]) in biodiesel was determined successfully using TLC analysis. When the results of the TLC analysis were compared with those of a chemical and enzymatic assay, the results were fairly similar. We conclude that TLC without additional analytical instruments can be used as an alternative method for the quantitative analysis of the concentration of glycerol in biodiesel.

TLC NAND-type Flash Memory Built-in Self Test (TLC NAND-형 플래시 메모리 내장 자체테스트)

  • Kim, Jin-Wan;Chang, Hoon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.12
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    • pp.72-82
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    • 2014
  • Recently, the size of semiconductor industry market is constantly growing, due to the increase in diffusion of smart-phone, tablet PC and SSD(Solid State Drive). Also, it is expected that the demand for TLC NAND-type flash memory would gradually increase, with the recent release of TLC NAND-type flash memory in the SSD market. There have been a lot of studies on SLC NAND flash memory, but no research on TLC NAND flash memory has been conducted, yet. Also, a test of NAND-type flash memory is depending on a high-priced external equipment. Therefore, this study aims to suggest a structure for an autonomous test with no high-priced external test device by modifying the existing SLC NAND flash memory and MLC NAND flash memory test algorithms and patterns and applying them to TLC NAND flash memory.

Effect of Injin Butanol Fraction with Thin Layer Chromatography on Fas-mediated Apoptosis (인진butanol 분획의 TLC추출성분이 Fas-mediated Apoptosis에 미치는 영향)

  • 박용진;김영철;이장훈;우흥정
    • The Journal of Korean Medicine
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    • v.23 no.2
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    • pp.57-69
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    • 2002
  • Objective: The aim of this study is to investigate the effect of Injin butanol fractions with Thin Layer Chromatography on Fas-mediated Apoptosis. Method: Injin-butanol fraction separated by TLC. MIT assay, cell cycle analysis, Caspase-3 protease assay, DNA fragmentation assay and quantitative RT-PCR were performed to evaluate the effects of TLC extraction of lnjin-butanol fraction on cell viability, cell cycle progression and apoptosis. Results: Scopoletin, luteolin, apigenin and unknown powder was isolated by TLC. Fas-mediated apoptosis analysis shows that scopoletin has inhibiting function on apoptosis. Caspase- 3 protease assay analysis shows that scopoletin inhibits activity of caspase-3. Quantitative RT-PCR analysis shows that no activity on caspase-3, but apoptosis inhibition cytokine -Bcl-2- is activated, and apoptosis activating cytokine -Bax- is unactivated. Conclusion: These results show that each fraction of Injin-butanol TLC extraction, especially scopoletin, acts as a protective function on liver cell viability, and inhibitory function on apoptosis. (J Korean Oriental Moo 2002;23(2):57-69)

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SLC Buffer Performance Improvement using Page Overwriting Method in TLC NAND Flash-based Storage Devices (TLC 낸드 플래시기반 저장 장치에서 페이지 중복쓰기 기법을 이용한 SLC 버퍼 성능향상 연구)

  • Won, Samkyu;Chung, Eui-Young
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.1
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    • pp.36-42
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    • 2016
  • In multi-level-cell based storage devices, TLC NAND has been employed solid state drive due to cost effectiveness. Since TLC has slow performance and low endurance compared with MLC, TLC based storage has adopted SLC buffer scheme to improve performance. To improve SLC buffer scheme, this paper proposes page overwriting method in SLC block. This method provides data updates without erase operation within a limited number. When SLC buffer area is filled up, FTL should execute copying valid pages and erasing it. The proposed method reduces erase counts by 50% or more compared with previous SLC buffer scheme. Simulation results show that the proposed SLC buffer overwrite method achieves 2 times write performance improvement.

Comparison of TLC and GC-MS Method in the Analysis of Dye Extracted from Madder Plant (꼭두서니 추출염료에 대한 TLC와 GC-MS 분석법의 비교 연구)

  • ;S. Kay Obendorf
    • The Research Journal of the Costume Culture
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    • v.12 no.4
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    • pp.579-590
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    • 2004
  • This research was aimed to investigate and compare the effectiveness of TLC and GC-MS methods in the analysis of chromophoric substances extracted from madder plant. Alizarin and purpurin 0.3% solution were used as comparative standards; madder extraction was prepared by heating the solution of powdered madder at 80℃, pH 1.5, for 90 min. Best elution solvent for TLC in silica gel plate was toluene:ethyl acetate=9:1, which resulted in red and yellow spots from madder extraction each of which showed R/sub f/ values 0.32-0.43 and 0.07-0.11. Although the red spot in particular exhibited similar characteristics as standard purpurin in color, shape, and R/sub f/ values, the result was inconsistent throughout different TLC trials. GC-MS analysis showed only small amount of alizarin and no purpurin in the madder extraction. Other chromophoric substance such as 2-furancarboxaldehyde, 5-(hydroxymethyl)-, anthralin, and danthron were also detected in small amounts. The result indicated that TLC was less sensitive to detecting and identifying the natural dyestuff which is generally constituted with a number of similar but chemically different chromophoric substances.

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The Effect of Sonicated Extracts of Treponema Denticola and Treponema Lecithinolyticum on the Cytokine Secretion and Matrix Metalloproteinase Activation of Gingival Fibroblast (Treponema denticola와 Treponema lecithinolyticum의 분쇄액이 치은섬유아세포의 Cytokine 분비 및 Matrix metalloproteinase 활성에 미치는 영향)

  • Suh, Hye-Yuhn;Choi, Bong-Kyu;Choi, Seong-Ho;Cho, Kyoo-Sung;Kim, Chong-Kwan;Chai, Jung-Kiu
    • Journal of Periodontal and Implant Science
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    • v.29 no.4
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    • pp.979-995
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    • 1999
  • This study was investigated to observe the effect of Treponema denticola cell sonicates(TDC) and Treponema lecithinolyticum cell sonicates(TLC) on cytokine secretion and matix metalloproteinase-2(MMP-2) activation of cultured human gingival fibroblast. Several experiments were performed including $IL-1{\beta}$, IL-6 ELISA for the effect on the $IL-1{\beta}$, IL-6 secretion of human gingival fibroblast. Also gelatinase zymography and gelatin dissolubility test for the activation of MMP-2 secreted by gingival fibroblast. The results were as follows. 1. The effect of TDC and TLC on IL-6 secretion of human gingival fibroblast showed statistically significant increase of IL-6 secretion in the TDC and TLC treated group compared to no treatment group(p<0.05) . 2. The amount of $IL-1{\beta}$ secretion was below the lower limit and there was no difference in the $IL-1{\beta}$ secretion of gingival fibroblast between TDC, TLC treated group and no treatment group. 3. The active form of pro MMP-2 with 72 kDa molecular weight was activated in both TDC and TLC treated group and clear band was appeared at 62kDa site on the zymography. 4. Gelatin dissolubility of MMP-2 secreted by gingival fibroblast was higher in TDC and TLC treated group compared to no treatment group(p<0.05). 5. In the TDC treated group, serine protease of T. denticola affect gelatin dissolubility. But in the TLC treated group gelatin was degraded by only MMP secreted by gingival fibroblast. Regarding to the above results, TDC and TLC have an effect on the IL-6 secretion increase of human gingival fibroblast and appears to activate pro MMP-2 which degrades collagen.

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Screening method for amines by derivatization reaction on TLC (TLC 상 유도체화 반응을 이용한 아민 계 화합물의 Screening 방법)

  • Choi, Sung-Woon;Lee, Hye-In;Sung, Nack-Do
    • Analytical Science and Technology
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    • v.26 no.4
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    • pp.228-234
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    • 2013
  • Methamphetamine is an amine-containing illegal drug and is distributed unlawfully in South Korea. Finding a rapid, convenient and semi-quantitative determination method for methamphetamine is a very important issue in the area of forensic drug testing. As an effort to develop new screening method, the reactions between three organic compounds which are structurally similar to methamphetamine and N-(9-fluorenylmethoxycarbonyloxy) succinimide (FMOC-NHS) were performed on silica gel ($SiO_2$) TLC plates. Three reference compounds were synthesized and used for the identification, comparison and study of the limit of detection (LOD) of the products obtained from a direct reaction on a TLC plate. As a result, FMOC-NHS as a derivatization reagent generated compounds containing highly UV-active functional groups on the TLC plate after reacting with primary- and secondary amines. In the experiment 2D the LOD of amines was in the range of 0.045 and 0.01 mg/mL ($2{\mu}L/spot$), and in 1D the LOD was in the range of 0.002 and 0.007 mg/mL ($2{\mu}L/spot$). The LODs of the compounds tested were dependent on the concentration of the derivatizing reagent.

Studies on the Analysis of Constituents of Deer Horn(I) -Assay of Trace Elements and TLC Pattern Analysis of Gangliosides- (산지별 녹용(鹿茸)류의 성분분석 연구(I) -미량금속의 정량 및 Gangliosides의 TLC Pattern 분석-)

  • Hong, Nam-Doo;Won, Do-Hee;Kim, Nam-Jae;Chang, Seung-Youb;Youn, Whang-Geum;Kim, Hae-Soo
    • Korean Journal of Pharmacognosy
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    • v.22 no.3
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    • pp.171-182
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    • 1991
  • Several experiments were carried for the purpose of establishing the basis for the quality evaluation of deer horn. Deer horn originated from China, New Zealand, Soviet and Alaska were used as objectives and some constituents involved in deer horn were assayed and compared with one another. Five kinds of trace elements including Ca and Fe were determined by atomic absorbance spectroscopy. The more the region came down from the top of the deer horn, the more the ratio of Ca to Fe content (Ca/Fe) was increased. The same increasing tendancy was observed in the case of ash content. Gangliosides were isolated from deer horn by alumina column chromatography and identified by TLC using gangliosides Type II obtained from bovine brain as the standard material. The TLC chromatograms were scanned by dual wavelength TLC scanner, then those TLC profiles were compared. Deer horn from China and New Zealand were analogous but those from Soviet and Alaska were each distinguished in their TLC profiles.

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