대한본초학회지 (The Korea Journal of Herbology)

  • 제35권6호
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  • Pages.43-53
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  • 2020
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
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대황(大黃)과 황금(黃芩) 추출물 혼합물이 급성 역류성 식도염 흰쥐에 미치는 효과

Effect of a Mixture of Rhei Rhizoma and Scutellariae Radix Extract on Acute Reflux Esophagitis Rats

  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이상남 (대구한의대학교 기공학교실) ;
  • 박순애 (대구한의대학교 한방식품조리영양학부) ;
  • 박해진 (DHU 바이오융복합시험센터)
  • Lee, Jin A (Department of Herbology, Korean Medicine of College, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, Korean Medicine of College, Daegu Haany University) ;
  • Lee, Sang-Nam (Department of Qigong, Daegu Haany University) ;
  • Park, Soon-Ae (Department of Herbal Food Cuisine & Nutrition, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center)
  • 투고 : 2020.10.06
  • 심사 : 2020.11.25
  • 발행 : 2020.11.30
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초록

Objective : Reflux esophagitis is a disease caused by reflux of stomach contents, stomach acid, and pepsin into the esophagus, and is currently increasing worldwide. This study was conducted to evaluate the effect of a mixture of Rhei Rhizoma and Scutellariae Radix (RS) extract on acute reflux esophagitis in rats. Methods : Rats were divided into five groups for examination: Normal group (Nor, n=8), water-treated acute reflux esophagitis rats (Con, n=8), tocopherol 30 mg/kg body weight/day-treated acute reflux esophagitis rats (Toco, n=8), RS 100 mg/kg body weight/day-treated acute reflux esophagitis rats (RS100, n=8), RS 200 mg/kg body weight/day-treated acute reflux esophagitis rats (RS200, n=8). All rats fasted for 18 h and then were derived by linking the metastatic junction between pylorus and forestomach and corpus. And rats were sacrificed 5 h after surgery. We analyzed the expression of NADPH, MAPK, inflammatory, anti-inflammatory, and tight junction related proteins by western blot in esophageal tissue and observed the level of reactive oxygen species (ROS), alanine aminotransferanse (ALT), and aspartate aminotransferase (AST) in serum. Results : RS administration significantly protected the esophageal mucosal damage of reflux esophagitis, and ROS, AST, and ALT levels were significantly reduced in RS administration compared to Con group. In addition, RS administration effectively suppressed MAPK and NF-κB pathways and upregulated protein expressions of tight junction protein. Conclusions : These results suggest that RS protected the esophageal mucosa by inhibiting the MAPK and NF-κB pathways and upregulating tight junctions.

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참고문헌

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