The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Podosphaera xanthii as the causal agent of powdery mildew disease affecting Echinacea purpurea in Korea

에키나시아 흰가루병을 일으키는 Podosphaera xanthii 동정

  • Choi, In-Young (Department of Agricultural Biology, Jeonbuk National University) ;
  • Hong, Sun-Hee (Department of Plant and Environment Science, Hankyong National University) ;
  • Lee, Yong-Ho (Department of Plant and Environment Science, Hankyong National University) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 최인영 (전북대학교 농생물학과) ;
  • 홍선희 (한경대학교 식물생명환경과학과) ;
  • 이용호 (한경대학교 식물생명환경과학과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Received : 2020.04.29
  • Accepted : 2020.08.27
  • Published : 2020.09.30
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Abstract

During the extensive forays for discovering the biodiversity of phytopathogenic fungi in Korea, powdery mildew-affected purple coneflowers (Echinacea purpurea) were frequently found. Since 2004, nine samples have been deposited in the Korea University Herbarium. The initial symptoms include white, evanescent mycelia and irregular patches on mature leaves. Mild symptoms in the form of discolored lesions are also noticed on the stem and petal of the affected plants. Based on the morphological characteristics of the anamorph and the phylogenetic analysis of the internal transcribed spacers (ITS) and large subunit (LSU) rDNA sequences, the causal fungus was identified as Podosphaera xanthii. This is the first report that identifies the causal agent of powdery mildew disease affecting purple coneflower in Korea.

한국에서 식물병원성 균류의 생물다양성을 파악하기 위한 균류 탐사를 통하여 에키나시아 흰가루병이 지속적으로 관찰되었다. 총 9점의 시료를 고려대학교 식물표본보관소에 보존하였다. 병든 식물에서는 주로 성엽에서 흰색의 얇은 균사층이 전면으로 또는 부분적으로 나타났다. 흰가루병균의 감염으로 줄기와 꽃잎에서는 변색 부위가 나타나는 특징을 보였다. 이 흰가루병균 무성세대의 형태적 특징과 더불어 ITS 및 LSU 염기서열을 계통분석한 결과, 이 흰가루병균은 Podosphaera xanthii로 동정되었다. 이는 한국에서 에키나시아 흰가루병에 관한 최초의 보고이다.

Keywords

References

  1. CABI. Echinacea purpurea [Internet]. Wallingford, : CAB International; 2020 [cited 2019 Nov 19]. Available from www.cabi.org/isc/datasheet/48033.
  2. Kumar KM, Ramaiah S. Pharmacological importance of Echinacea purpurea. Intl J Pharm Biosci 2011;2:304-14.
  3. Gu Y, Maenaka T, Saito K, Yamashita T, Choi IS, Terai K, Ahn KS. Antioxidant and immunoenhancing effects of Echinacea purpurea (American herb) in vivo. Orient Pharm Exp Med. 2005;5:48-56. https://doi.org/10.3742/OPEM.2005.5.1.048
  4. Sheshbahreh MJ, Dehnavi MM, Salehi A, Bahreininejad B. Physiological and yield responses of purple coneflower (Echinacea purpurea) to nitrogen sources at different level of irrigation. Physiol Mol Biol Plants. 2019;25:177-87. https://doi.org/10.1007/s12298-018-0611-7
  5. Farr DF, Rossman AY. Fungal databases, U.S. National Fungus Collections [Internet]. Beltsville: Systematic Mycology and Microbiology Laboratory; 2020 [cited 2020 Sep 1]. Available from: http://nt.ars-grin.gov/fungaldatabases.
  6. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  7. Braun U, Cook RT. Taxonomic manual of the Erysiphales (powdery mildews). Utrecht: CBSKNAW Fungal Biodiversity Centre; 2012.
  8. White TJ, Bruns T, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, ed. by Innis MA, Gelfand DH, Sninsky JJ, White TJ ediotrs. San Diego: Academic Press; 1990. P. 315-322.
  9. Takamatsu S, Kano Y. PCR primers useful for nucleotide sequencing of rDNA of the powdery mildew fungi. Mycoscience 2001;42:135-39. https://doi.org/10.1007/BF02463987
  10. Khodaparast SA, Takamatsu S, Hedjaroude GA. Phylogenetic analysis of Iranian powdery mildew fungi using nucleotide sequences of 28S ribosomal DNA. J Agric Sci Technol 2005;7:49-58.
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  12. Amano K. Host range and geographical distribution of the powdery mildew fungi. Tokyo: Japan Sci. Soc. Press; 1986.
  13. Kiss L, Vaghefi N, Bransgrove K, Dearnaley JDW, Takamatsu S, Tan YP, Marston C, Liu SY, Jin DN, Adorada DL, et al. Australia: A continent without native powdery mildews? The first comprehensive catalog indicates recent introductions and multiple host range expansion events, and leads to the re-discovery of Salmonomyces as a new lineage of the Erysiphales. Front Microbiol 2020;11:1571. https://doi.org/10.3389/fmicb.2020.01571
  14. Sholberg PL, Ginns JH, Li TSC. First report of powdery mildew caused by Erysiphe cichoracearum on coneflowers. Plant Dis 1999;83:694. https://doi.org/10.1094/PDIS.1999.83.7.694B
  15. Garibaldi A, Bertetti S, Matic S, Guillino ML. Powdery mildew caused by Golovinomyces cichoracearum on Eastern purple coneflower (Echinacea purpurea ) in Italy. Plant Dis 2018;102:1028. https://doi.org/10.1094/PDIS-08-17-1220-PDN