Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Status of Molecular Biotechnology Research Based on Tissue Culture of Soybean

콩 조직배양 기술에 기반한 생명공학 연구 동향

  • Seo, Mi-Suk (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Cho, Chuloh (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Choi, Man-Soo (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Chun, JaeBuhm (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Jin, Mina (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Kim, Dool-Yi (Crop Foundation Research Division, National Institute of Crop Science)
  • 서미숙 (국립식량과학원 작물기초기반과) ;
  • 조철오 (국립식량과학원 작물기초기반과) ;
  • 최만수 (국립식량과학원 작물기초기반과) ;
  • 전재범 (국립식량과학원 작물기초기반과) ;
  • 진민아 (국립식량과학원 작물기초기반과) ;
  • 김둘이 (국립식량과학원 작물기초기반과)
  • Received : 2020.06.04
  • Accepted : 2020.09.28
  • Published : 2020.10.01
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Abstract

Soybean (Glycine max (L.) Merrill) is one of the most important crops of the world. With the completion of the soybean genome sequence, the Korean soybean core collection consisted of 430 accessions with genetic and phenotypic diversity was constructed in recent year. The availability of genome sequences and core collection will result in the crop improvement by molecular breeding using the various accessions and genome editing approaches. Efficient tissue culture techniques, such as haploid production, protoplast culture and plant regeneration from various organs are essential for the successful molecular biological approach and crop improvement. However, soybean is still considered to be recalcitrant in tissue culture because of the low frequency of regeneration and limitation of available responsive cultivars. In this study, we discuss the recent studies of tissue culture technology and methodology for efficient tissue culture to genetic improvement and application of molecular biotechnology in soybean.

콩은 전세계적으로 재배되는 중요한 작물 중에 하나로 최근, 표준유전체 해독과 함께 유전적, 표현형적으로 다양성을 가진 한국핵심집단이 구축됨에 따라 유전체 기반 분자 육종 연구, 유전자 교정 기술을 활용한 새로운 육종 소재 개발 연구가 가속화될 것으로 예상된다. 유전체 정보 기반 작물의 분자 육종 및 생명공학 연구를 통한 성공적인 작물의 개량을 위해서는 식물의 효율적인 조직배양 기술이 수반되어야 할 것이다. 그러나 반수체 생산, 원형질체 배양 및 형질전환 기술과 같은 콩의 조직배양 효율은 아직까지 높지 않고 일부 계통에 한정되어 이루어지고 있다. 본 논문에서는 콩의 분자육종 및 생명공학 기술의 적용을 위하여 다양한 콩 조직배양 기술에 관한 연구 동향을 분석하고 조직배양 효율에 영향을 미치는 요인들에 대한 정보를 제공하고자 하였다.

Keywords

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