Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Development of microsatellite markers for Hosta capitata (Asparagaceae) and amplification in related taxa

  • CHOI, Mi-Jung (Department of Biology and Chemistry, Changwon National University) ;
  • LEE, Jung-Hyun (Department of Biology Education, Chonnam National University) ;
  • CHO, Won-Bum (Department of Biology Education, Chonnam National University) ;
  • HAN, Eun-Kyeong (Department of Biology Education, Chonnam National University) ;
  • CHOI, Hyeok-Jae (Department of Biology and Chemistry, Changwon National University)
  • Received : 2020.06.22
  • Accepted : 2020.09.14
  • Published : 2020.09.30
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Abstract

Microsatellite markers were developed as a tool for phylogeographic studies of Hosta capitata. We also assessed cross-amplification in species closely related to Hosta capitata. We produced 28 polymorphic microsatellite markers by mapping 300 bp paired-end reads obtained from Illumina MiSeq data of H. capitata. In H. capitata, the number of alleles per locus ranged from 1 to 13. Observed and expected heterozygosity ranged from 0.000 to 0.844 and 0.000 to 0.832, respectively. Additionally, 13 loci were successfully transferable to the related species of H. minor and H. venusta. These markers will provide a powerful genetic tool not only for elucidating the phylogeographic patterns of H. capitata populations but also for studying the genetic delimitation of H. capitata from its related species.

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References

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