Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of Physical Properties and Biocompatibility of HA-Dex Fusion Hydrogel Patch for Atopic Healing Ability

HA-Dex 융복합 하이드로겔 패치의 아토피 치유 능력에 대한 물리적 특성 및 생체 적합성 평가

  • Hong, Gyeong Sik (Department of Bio-fibers and Materials Science, Kyungpook National University) ;
  • Choi, Jeong Yeon (Safety System R&D Group, Korea Institute of Industrial Technology (KITECH)) ;
  • Choi, Jin Hyun (Department of Bio-fibers and Materials Science, Kyungpook National University)
  • 홍경식 (경북대학교 바이오섬유소재학과) ;
  • 최정연 (한국생산기술연구원 안전시스템연구그룹) ;
  • 최진현 (경북대학교 바이오섬유소재학과)
  • Received : 2020.06.10
  • Accepted : 2020.09.15
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, since atopic dermatitis is sensitive to skin irritation, it has been suggested that the development of a patch that can effectively exhibit adhesion and absorption to a specific local area while minimizing skin irritation, and capable of appropriate drug release should be given priority. In this study, we tried to develop a hydrogel patch that minimizes skin irritation, adheres effectively to a specific area, and promotes absorption. The atopic patch was formulated into a super-absorbent hydrogel sheet using a freeze drying method. Cell viability assay was carried out using keratinocytes (HaCaT cell) and fibroblasts (L929 cells). In order to investigate the physical properties, FT-IR, FE-SEM, porosity analysis and swelling behavior were investigated. As a result, the newly prepared HA-Dex hydrogel patch was verified by biocompatibility and physical evaluation. In addition, the manufactured hydrogel patch has sufficient moisture absorption capacity and can relieve itching of atopic skin, and is expected to be applied to various drug delivery products for the treatment of atopic dermatitis in the future.

최근, 아토피 피부염은 피부 자극에 민감하므로 피부 자극을 최소화하면서도, 특정한 국소 부위에 대한 점착력과 흡수력을 효과적으로 발휘할 수 있으며 적절한 약물 방출을 거동할 수 있는 패치 개발이 우선시 되어야 한다고 제안되기도 하였다. 따라서 본 연구는 피부 자극을 최소화하고 특정 부위에 효과적으로 접착 및 흡수 할 수 있는 하이드로겔 패치를 개발코자 하였다. 아토피 패치는 동결 건조법을 이용하여 고흡수성 하이드로겔 시트를 제형화 하였다. 인간각질세포(HaCaT cells) 및 섬유아세포(L929 cells) 사용하여 세포 안정성을 수행하였다. 물리적 성질을 조사하고자 FT-IR, FE-SEM, 다공성 분석, 팽윤성 거동을 조사 하였다. 그 결과, 새롭게 제조된 HA-Dex 하이드로겔 패치는 생체 적합성 및 물리적 평가에 의해 입증하였다. 또한 제조된 하이드로겔 패치는 충분한 수분 흡수력과 아토피성 피부의 가려움증을 완화시킬 수 있으며, 향후 아토피 성 피부염 치료에 다양한 약물 전달 제품에 적용될 수 있을 것으로 기대된다.

Keywords

References

  1. A . P. Grammatikos, The genetic and environmental basis of atopic diseases, Ann Med., 40(7), 482 (2008). https://doi.org/10.1080/07853890802082096
  2. S. Weidinger and N. Novak, Atopic dermatitis, Lancet, 387(10023), 1109 (2016). https://doi.org/10.1016/S0140-6736(15)00149-X
  3. Y. M. Park, Advances in the pathophysiology of atopic dermatitis, Allergy Asthma & Respir Dis., 16(3), 189 (2006).
  4. Ye. S, Mo. X, Liu. J, Yan. F, and Chen. D, Factors influencing atopic dermatitis incidence in offspring, Iran J Allergy Asthma Immunol., 18(4), 347 (2019).
  5. R. J. Milieu. Toncic, I. Jakasa, S.L. Hadzavdic, S. M. Goorden, K.J.G. Vlugt, F.S. Stet, A. Balic, M. Petkovic, B. Pavicic, K. Zuzul, B. Marinovic, and S. Kezic, Altered levels of sphingosine, sphinganine and their ceramides in atopic dermatitis are related to skin barrier function, disease severity and local cytokine, Int J Mol Sci., 21(6), 1958 (2020). https://doi.org/10.3390/ijms21061958
  6. J. F. Scott, R. R. Z. Conic, I. Kim, D. Y. Rowland, and S. T. Nedorost, Atopy and sensitization to allergens known to cause systemic contact dermatitis, Dermatitis, 30(1), 62 (2019). https://doi.org/10.1097/DER.0000000000000436
  7. J. Chen, S. Jo, and K. Park, Polysaccharide hydrogels for protein drug delivery, Carbohydrate Polymers, 28(1), 69 (1995). https://doi.org/10.1016/0144-8617(95)00080-1
  8. G. Sun, X . Zhang, Y. I . Shen, R . S ebastian, L . E . Dickinson, K. Fox-Talbot, M. Reinblatt, C. Steenbergen, J. W. Harmon, a nd S. Gerecht, Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing, Proc Natl Acad Sci U S A., 108(52), 20976 (2011). https://doi.org/10.1073/pnas.1115973108
  9. G. D. Prestwich, D. M. Marecak, J. F. Marecek, K. P. Vercruysse, and M. R. Ziebell, Controlled chemical modification of hyaluronic acid: Synthesis, applications, and biodegradation of hydrazide derivates, Journal of Controlled Release, 53(1-3), 93 (1998). https://doi.org/10.1016/S0168-3659(97)00242-3
  10. F. Gao, Y. Liu, Y. He, C . Yang, Y . Wang, X . Shi, and G. Wei, Hyaluronan oligosaccharide promote excisional wound healing through enhanced angiogenesis, Matrix Biology, 29(2), 107 (2010). https://doi.org/10.1016/j.matbio.2009.11.002
  11. K. S. Kim, Y. J. Lee, W. S. Lyoo, and S . K. Noh, Preparation of high molecular weight atactic poly(vinyl alcohol) hydrogel by electron beam irradiation technique, Polymer Korea, 32(6), 587 (2008).
  12. M. Slevin, J. Krupinski, and J. Gaffney, S. Matou, D. West, H. Delisser, R. C. Savani, and S. Kumar, Hyaluronan-mediated angiogenesis in vascular disease: Uncovering RHAMM and CD44 receptor signaling pathways, Matrix Biology, 26(1), 58 (2007). https://doi.org/10.1016/j.matbio.2006.08.261