International Journal of Advanced Culture Technology

  • 제8권3호
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  • Pages.231-240
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  • 2020
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  • 2288-7202(pISSN)
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  • 2288-7318(eISSN)
국제문화기술진흥원 (The International Promotion Agency of Culture Technology)
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Antimicrobial, Antioxidative, Elastase and Tyrosinase Inhibitory Effect of Supercritical and Hydrothermal Asparagopsis Armata Extract

  • Lee, Kwang Won (Department of Beauty & Cosmetic Science, Eulji Univ.) ;
  • Heo, Soo Hyeon (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Lee, Jinseo (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Park, Su In (Department of Senior Healthcare majoring in Cosmetic Pharmacology, Eulji Univ.) ;
  • Kim, Miok (Department of Beauty Design, Shin Ansan Univ.) ;
  • Shin, Moon Sam (Department of Senior Healthcare and Beauty & Cosmetic Science, Eulji Univ.)
  • 투고 : 2020.07.30
  • 심사 : 2020.09.02
  • 발행 : 2020.09.30
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초록

In this paper, we present to evaluate physiological activity of Asparagopsis armata extraction. After extraction with Asparagopsis armata using hydrothermal and supercritical carbon dioxide, various physiological activities were examined. The total concentration of polyphenol compounds was determined to be 18.85 mg/g of hydrothermal Asparagopsis armata extraction and 14.74 mg/g of supercritical Asparagopsis armata extraction. In DPPH radical scavenging assay, ascorbic acid was used as positive antioxidant control. In ABTS radical scavenging assay, ascorbic acid was used as positive antioxidant control. The percentage of inhibition and IC50 were measured. The IC50 of Asparagopsis armata extraction is 261.44ppm and the IC50 of supercritical Asparagopsis armata extraction is 153.98 ppm. The elastase inhibitory assay showed concentration dependence and the IC50 of hydrothermal Asparagopsis armata extraction is 3387 ppm and the IC50 of supercritical Asparagopsis armata extraction is higher than 2500 ppm. In mushroom tyrosinase inhibition experiments, tyrosinase inhibition's IC50 of supercritical Asparagopsis armata extraction was 248.06. In the SOD-like experiments, the concentration-dependent results were showed and IC50 of hydrothermal Asparagopsis armata extraction is 845.29 ppm. In the antimicrobial experiments, maximum clear zones of supercritical Asparagopsis armata extraction represented 23.00 mm in Propionibacterium acnes. In the other hand, in experiments with the same conditions, hydrothermal Asparagopsis armata extraction had no effect in all strains.

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