Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Enhancer Function of MicroRNA-3681 Derived from Long Terminal Repeats Represses the Activity of Variable Number Tandem Repeats in the 3' UTR of SHISA7

  • Lee, Hee-Eun (Department of Integrated Biological Science, Pusan National University) ;
  • Park, Sang-Je (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Huh, Jae-Won (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Imai, Hiroo (Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University) ;
  • Kim, Heui-Soo (Institute of Systems Biology, Pusan National University)
  • Received : 2020.03.04
  • Accepted : 2020.05.27
  • Published : 2020.07.31
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Abstract

microRNAs (miRNAs) are non-coding RNA molecules involved in the regulation of gene expression. miRNAs inhibit gene expression by binding to the 3' untranslated region (UTR) of their target gene. miRNAs can originate from transposable elements (TEs), which comprise approximately half of the eukaryotic genome and one type of TE, called the long terminal repeat (LTR) is found in class of retrotransposons. Amongst the miRNAs derived from LTR, hsa-miR-3681 was chosen and analyzed using bioinformatics tools and experimental analysis. Studies on hsa-miR-3681 have been scarce and this study provides the relative expression analysis of hsa-miR-3681-5p from humans, chimpanzees, crab-eating monkeys, and mice. Luciferase assay for hsa-miR-3681-5p and its target gene SHISA7 supports our hypothesis that the number of miRNA binding sites affects target gene expression. Especially, the variable number tandem repeat (VNTR) and hsa-miR-3681-5p share the binding sites in the 3' UTR of SHISA7, which leads the enhancer function of hsamiR-3681-5p to inhibit the activity of VNTR. In conclusion, hsa-miR-3681-5p acts as a super-enhancer and the enhancer function of hsa-miR-3681-5p acts as a repressor of VNTR activity in the 3' UTR of SHISA7.

Keywords

References

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