Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Speciation Analysis of Seleno-Compounds in Hair and Finger Nails

손톱과 모발에서의 셀레노 화합물의 화학종 분석

  • Ji, Young (Department of Chemistry Education, Korea National University of Education) ;
  • Keum, Daeseop (Department of Chemistry Education, Korea National University of Education) ;
  • Pak, Yong N. (Department of Chemistry Education, Korea National University of Education)
  • 지영 (한국교원대학교 화학교육학과) ;
  • 금대섭 (한국교원대학교 화학교육학과) ;
  • 박용남 (한국교원대학교 화학교육학과)
  • Received : 2020.03.04
  • Accepted : 2020.04.28
  • Published : 2020.08.20
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Abstract

Selenium species in hair and nails were extracted, separated and analyzed with HPLC-ICP/MS. The result showed that Se species was mostly SeCys and the information related to the change of selenium concentration in a body could be obtained. The Se fortification study was performed for 5 participants during 12 months. The average of Se concentration was 858.6 ± 201.2 ng g-1, which did not change much during the research period nor the difference between the individuals was striking. In the case of hair, the average concentration was 205.3 ng g-1 which was lower than nails. Se supplement of 22 ㎍ day-1 was taken for 3 months by the volunteers and the difference between before and after (3 months period for each) the taking of supplement was not striking. However, when the dose was increased to 300 ㎍ day-1 for 2 months, the concentration was increased noticeably to 1,230 ng g-1. It can be thought that the analysis of finger nail for Se can provide valuable information for the long period (several months) of exposure or intake of Se for a human body.

손톱과 모발에서 셀레늄을 추출하고 HPLC-ICP/MS로 셀레늄의 각 화학종을 분리한 뒤 분석하였다. 셀레노 화합물은 주로 SeCys으로 구성되어 있음을 알아내었고 이 들 시료의 분석을 통하여 인체에서의 셀레늄에 농도의 변화에 대한 정보를 얻을 수 있었다. 5명의 참가자들에 대한 손톱을 12개월간 모니터링한 결과, 농도는 평균 858.6 ± 201.2 ng g-1 이었는데 각 개인의 농도는 시간에 따라 변화가 크지 않고 일정하게 유지되었으며 개인간의 평균값차이도 심하지 않았다. 모발의 경우, 평균농도는 205.3 ng g-1으로서 손톱보다는 낮았다. 지원자들에게 셀레늄 보조제 22 ㎍ day-1를 3개월씩 투여하였을 때에 섭취 전후(각 3개월의 기간)의 농도는 크게 변화하지 않았지만 셀레늄보조제의 농도를 300 ㎍ day-1으로 증가시켜 2개월간 투여하였을 때에는 손톱에서의 셀레늄 농도가 1,230 ng g-1으로 현저히 증가하는 것으로 나타났다. 결론적으로 손톱은 인체가 장기간(수 개월 이상)동안 노출되었거나 또는 흡수한 셀레늄에 관한 정보를 반영하여 나타낼 수 있음을 본 연구를 통하여 알아낼 수 있었다.

Keywords

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