Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Comparative proteomic analysis of Celastrus hindsii Benth. phenotypes reveals an intraspecific variation

  • Nguyen, Van Huy (Institute of Applied Research and Development, Hung Vuong University) ;
  • Pham, Thanh Loan (Institute of Applied Research and Development, Hung Vuong University) ;
  • Ha, Thi Tam Tien (Institute of Applied Research and Development, Hung Vuong University) ;
  • Hoang, Thi Le Thu (Institute of Applied Research and Development, Hung Vuong University)
  • Received : 2020.11.04
  • Accepted : 2020.12.02
  • Published : 2020.12.31
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Abstract

In Vietnam, Celastrus hindsii Benth, a medicinal plant rich in secondary metabolites, has been used to alleviate distress caused by ulcers, tumors, and inflammation for generations. The occurrence of two phenotypes, Broad Leaf (BL) and Narrow Leaf (NL), has raised questions about the selection of appropriate varieties for conservation and crop improvement to enhance medicinal properties. This study examined molecular differences in C. hindsii by comparing protein profiles between the NL and BL types using 2D-PAGE and MS. Peptide sequences and proteins were identified by matching MS data against the MSPnr100 databases and verified using the MultiIdent tool on ExPASy and the Blast2GO software. Our results revealed notable variations in protein abundance between the NL and BL proteomes. Selected proteins were confidently identified from 12 protein spots, thereby highlighting the molecular variation between NL and BL proteomes. Upregulated proteins in BL were found to be associated with flavonoid and amino acid biosynthesis as well as nuclease metabolism, which probably attributed to the intraspecific variations. Several bioactive proteins identified in this study can have applications in cancer therapeutics. Therefore, the BL phenotype characterized by healthier external morphological features has higher levels of bioactive compounds and could be better suited for medicinal use.

Keywords

Acknowledgement

The authors are grateful to the Ministry of Education and Training for financial assistance and colleagues from the Institute of Applied Research and Development, Hung Vuong University, Vietnam for technical support during the project.

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