Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of Yijin-tang on Pacemaker Potentials in Interstitial Cells of Cajal of Murine Small Intestine

이진탕의 생쥐 소장 카할세포 향도잡이 전압에 미치는 효능에 관한 연구

  • Han, Donghun (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Kim, Jeong Nam (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Kim, Byung Joo (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
  • 한동훈 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김정남 (부산대학교 한의학전문대학원 양생기능의학부) ;
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학부)
  • Received : 2020.01.23
  • Accepted : 2020.02.26
  • Published : 2020.02.28
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Abstract

Obejectives : The purpose of this study was to investigate the effects of Yijin-tang on pacemaker potentials of small intestinal interstitial Cells of Cajal (ICC). Methods : To dissociate the ICC, we used enzymatic digestions from the small intestine in mice. The electrophysiological whole-cell patch-clamp configuration was used to record pacemaker potentials in the cultured ICC and the in vivo effects of Yijin-tang on GI motility were investigated by calculating percent intestinal transit rates (ITR). Results : 1. The ICC generated pacemaker potentials in the murine small intestine. Yijin-tang produced membrane depolarization with concentration-dependent manners in the current clamp mode. 2. Pretreatment with a Ca2+ free solution and thapsigargin, a Ca2+-ATPase inhibitor in the endoplasmic reticulum, stopped the pacemaker potentials. In the case of Ca2+-free solutions and thapsigargin, Yijin-tang did not induce membrane potential depolarizations. 3. U73122, a phospholipase C (PLC) inhibitors, blocked the Yijin-tang-induced membrane potential depolarizations. However, U73343, an inactive PLC inhibitors, did not block. 4. In the presence of protein kinase C (PKC) inhibitors, staurosporine or Rottlerin, Yijin-tang depolarized the pacemaker potentials. However, in the presence of Go6976, Yijin-tang did not depolarize the pacemaker potentials. 5. In mice, intestinal transit rate (ITR) values were significantly and dose-dependently increased by the intragastric administration of Yijin-tang. Conclusions : These results suggest that Yijin-tang can modulate the pacemaker activity of ICC through an internal/external Ca2+ and PLC/PKC-dependent pathway in ICC. In addition, Yijin-tang is a good candidate for the development of a prokinetic agent.

Keywords

References

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