New & Renewable Energy (신재생에너지)

Korean Society for New and Renewable Energy (한국신·재생에너지학회)
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Microbial Enrichment and Community Analysis for Bioelectrochemical Acetate Production from Carbon Dioxide

이산화탄소로부터 생물전기화학적 아세트산 생산을 위한 미생물 농화배양 및 군집 분석

  • Kim, Junhyung (School of Chemical & Biomolecular Engineering, Pusan National University) ;
  • Kim, Young-Eun (School of Chemical & Biomolecular Engineering, Pusan National University) ;
  • Park, Myeonghwa (Department of Civil & Environmental Engineering, Pusan National University) ;
  • Song, Young Eun (School of Chemical & Biomolecular Engineering, Pusan National University) ;
  • Seol, Eunhee (School of Chemical & Biomolecular Engineering, Pusan National University) ;
  • Kim, Jung Rae (School of Chemical & Biomolecular Engineering, Pusan National University) ;
  • Oh, You-Kwan (School of Chemical & Biomolecular Engineering, Pusan National University)
  • Received : 2019.11.13
  • Accepted : 2020.01.02
  • Published : 2020.03.25
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Abstract

Microbial electrosynthesis has recently been considered a potentially sustainable biotechnology for converting carbon dioxide (CO2) into valuable biochemicals. In this study, bioelectrochemical acetate production from CO2 was studied in an H-type two-chambered reactor system with an anaerobic microbial consortium. Metal-rich mud flat was used as the inoculum and incubated electrochemically for 90 days under a cathode potential of -1.1 V (vs. Ag/AgCl). Four consecutive batch cultivations resulted in a high acetate concentration and productivity of 93 mmol/L and 7.35 mmol/L/day, respectively. The maximal coulombic efficiency (rate of recovered acetate from supplied electrons) was estimated to be 64%. Cyclic voltammetry showed a characteristic reduction peak at -0.2~-0.4 V, implying reductive acetate generation on the cathode electrode. Furthermore, several electroactive acetate-producing microorganisms were identified based on denaturing- gradient-gel-electrophoresis (DGGE) and 16S rRNA sequence analyses. These results suggest that the mud flat can be used effectively as a microbial source for bioelectrochemical CO2 conversion.

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References

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