Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Angular-based Measurement for Quantitative assay of Albumin in three-dimensional Paper-based analytical Device

회전각도를 이용한 알부민 농도 측정용 3차원 종이 칩

  • Kim, Dong-Ho (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Jeong, Seong-Geun (Department of Chemical Engineering and Applied Chemistry, Chungnam National University) ;
  • Lee, Chang-Soo (Department of Chemical Engineering and Applied Chemistry, Chungnam National University)
  • 김동호 (충남대학교 응용화학공학과) ;
  • 정성근 (충남대학교 응용화학공학과) ;
  • 이창수 (충남대학교 응용화학공학과)
  • Received : 2020.01.19
  • Accepted : 2020.02.14
  • Published : 2020.05.01
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Abstract

This study presents an angular-based measurement on three-dimensional paper-based analytical devices (3D-PADs) for quantitative detection of albumin without using an image analyzer. We demonstrate a simple quantitative and straightforward approach based on the angle of the discolored area as detection criteria. 3D-PADs are rapidly fabricated by the wax-printing and laminating process. The 3D-PADs are treated with citrate buffer and tetrabromophenol blue to react with albumin in a sample solution. Dropping sample solution into sample pad in the 3D-PAD, fluid flows toward the assay zone laterally and vertically by capillary action. We find that the change of angle of the discolored area correctly reflects the concentration of albumin and is reliable determinant for the measurement of the albumin concentration. It is the first demonstration of angular-based detection as a simple, inexpensive, and equipment-free approach for point-of-care diagnosis.

본 연구에서는 별도의 이미지 분석 장비를 사용하지 않고 정량적으로 알부민의 농도를 측정할 수 있는 회전각도를 이용한 3차원 종이 칩(3D-PADs)를 제시한다. 변색된 구간의 회전각도를 측정하는 간단한 방법으로 검출을 시연하였다. 3D-PADs는 왁스 프린터를 이용한 인쇄와 라미네이팅 과정을 거쳐 빠르게 제작할 수 있다. 3D-PADs는 샘플의 알부민을 검출하기 위하여 citrate buffer와 tetrabromophenol blue를 흡수시켰다. 3D-PAD의 흡수패드에 샘플 용액을 흡수시키면, 샘플 용액은 형성된 유로를 통하여 수직 및 수평 흐름을 통해 분석 구간으로 흐른다. 변색된 구간의 회전각도는 특정한 알부민의 농도를 나타내며, 알부민 측정의 신뢰할 수 있는 값임을 확인할 수 있었다.

Keywords

References

  1. Park, Y. and Lee, H., "The Levels of Physical Activity and Its Relationships with Depression, Health-related Quality of Life, Sleep Disturbance, and Physiological Indicators in Hemodialysis Patients," Korean Journal of Adult Nursing, 27(6), 718-727 (2015). https://doi.org/10.7475/kjan.2015.27.6.718
  2. Na, K., "New Oral Agent for Treatment of Anemia in Patient with Chronic Kidney Disease: Prolyl Hydroxylase Inhibitor," The Korean Journal of Medicine, 94(1), 11-16(2019). https://doi.org/10.3904/kjm.2019.94.1.11
  3. Gervais, L., De Rooij, N. and Delamarche, E., "Microfluidic Chips for Point of Care Immunodiagnostics," Advanced Materials, 23(24), H151-H176(2011). https://doi.org/10.1002/adma.201100464
  4. Chin, C. D., Linder, V. and Sia, S. K., "Lab-on-a-chip Devices for Global Health: Past Studies and Future Opportunities," Lab on a Chip, 7(1), 41-57(2007). https://doi.org/10.1039/B611455E
  5. Peeling, R. W., Holmes, K. K., Mabey, D. and Ronald, A., "Rapid Tests for Sexually Transmitted Infections (STIs): the Way Forward," Sexually Transmitted Infections, 82(suppl 5), v1-v6(2006). https://doi.org/10.1136/sti.2006.024265
  6. Christwardana, M., Chung, Y., Tannia, D. C. and Kwon, Y. "Effects of the Gold Nanoparticles Including Different Thiol Functional Groups on the Performances of Glucose-oxidase-based Glucose Sensing Devices," Korean Journal of Chemical Engineering, 35(12), 2421-2429(2018). https://doi.org/10.1007/s11814-018-0163-0
  7. Park, S. J., Kim, S., Kim, S. H., Park, K. M. and Hwang, B. H. "His-tagged Protein Immobilization on Cationic Ferrite Magnetic Nanoparticles," Korean Journal of Chemical Engineering, 35(6), 1297-1302(2018). https://doi.org/10.1007/s11814-018-0043-7
  8. Son, J., Hwang, J., Lee, D., Khan, M. S., Jo, Y., Lee, K., Park, C., Chavan, S., Seo, Y. and Choi, Y., "Strategies for the Optimization of Bead-immunoassays for the Effective Detection of Target Biomolecules," Korean Journal of Chemical Engineering, 35(3), 805-811(2018). https://doi.org/10.1007/s11814-017-0323-7
  9. Lee, M., Kim, J.-H. and Koo, H.-J., "Dielectrophoresis for Control of Particle Transport: Theory, Electrode Designs and Applications," Korean Chemical Engineering Research, 57(2), 149-163 (2019).
  10. Heo, N. S., Oh, S. Y., Ryu, M. Y., Baek, S. H., Park, T. J., Choi, C., Huh, Y. S. and Park, and J. P., "Affinity Peptide-guided Plasmonic Biosensor for Detection of Noroviral Protein and Human Norovirus," Biotechnology and Bioprocess Engineering, 24(2), 318-325(2019). https://doi.org/10.1007/s12257-018-0410-6
  11. Zhou, M., Yang, M. and Zhou, F., "Paper Based Colorimetric Biosensing Platform Utilizing Cross-linked Siloxane as Probe," Biosensors and Bioelectronics, 55, 39-43(2014). https://doi.org/10.1016/j.bios.2013.11.065
  12. Mu, X., Zhang, L., Chang, S., Cui, W. and Zheng, Z., "Multiplex Microfluidic Paper-based Immunoassay for the Diagnosis of Hepatitis C Virus Infection," Analytical Chemistry, 86(11), 5338-5344(2014). https://doi.org/10.1021/ac500247f
  13. Hong, W., Jeong, S.-G., Shim, G., Kim, D. Y., Pack, S. P. and Lee, C.-S., "Improvement in the Reproducibility of a Paper-based Analytical Device (PAD) Using Stable Covalent Binding between Proteins and Cellulose Paper," Biotechnology and Bioprocess Engineering, 23(6), 686-692(2018). https://doi.org/10.1007/s12257-018-0430-2
  14. Maharjan, A., Alkotaini, B. and Kim, B. S. "Fusion of Carbohydrate Binding Modules to Bifunctional Cellulase to Enhance Binding Affinity and Cellulolytic Activity," Biotechnology and Bioprocess Engineering, 23(1), 79-85(2018). https://doi.org/10.1007/s12257-018-0011-4
  15. Shin, J.-W., Yoon, J., Shin, M., Choi, and J.-W., "Electrochemical Dopamine Biosensor Composed of Silver Encapsulated $MoS_2$ Hybrid Nanoparticle," Biotechnology and Bioprocess Engineering, 24(1), 135-144(2019). https://doi.org/10.1007/s12257-018-0350-1
  16. Hinberg, I. H., Katz, L. and Waddell, L. "Sensitivity of in vitro Diagnostic Dipstick Tests to Urinary Protein," Clinical Biochemistry, 11(2), 62-64(1978). https://doi.org/10.1016/S0009-9120(78)90719-1
  17. Bae, E., Park, H.-J., Yoon, J., Kim, Y., Choi, K. and Yi, J., "Bacterial Uptake of Silver Nanoparticles in the Presence of Humic Acid and $AgNO_3$," Korean Journal of Chemical Engineering, 28(1), 267-271(2011). https://doi.org/10.1007/s11814-010-0351-z
  18. Yu, J. H., Jeong, S.-G., Lee, C.-S., Hwang, J.-Y., Kang, K.-T., Kang, H. and Lee, S.-H., "Fabrication of a Paper-based Analytical Device for Multiple Colorimetric Analysis via Inkjet-printing and Paper-cutting," BioChip Journal, 9(2), 139-143(2015). https://doi.org/10.1007/s13206-015-9207-2
  19. Kolthoff, I. M. and Elving, P. J., Treatise on Analytical Chemistry, Part 1 (Vol. 13). John Wiley & Sons(1993).
  20. Duy, L. T., Trung, T. Q., Dang, V. Q., Hwang, B.-U., Siddiqui, S., Son, I.-Y., Yoon, S. K., Chung, D. J. and Lee, N.-E., "Flexible Transparent Reduced Graphene Oxide Sensor Coupled with Organic Dye Molecules for Rapid Dual-Mode Ammonia Gas Detection," Advanced Functional Materials, 26(24), 4329-4338(2016). https://doi.org/10.1002/adfm.201505477
  21. Samiey, B., Alizadeh, K., Mousavi, M. F. and Alizadeh, N. "Study of Kinetics of Bromophenol Blue Fading in Alcohol-water Binary Mixtures by Sesmortac Model," Bulletin of the Korean Chemical Society, 26(3), 384-392(2005). https://doi.org/10.5012/bkcs.2005.26.3.384
  22. Gohain, B. and Dutta, R. K., "Premicellar and Micelle Formation Behavior of Dye Surfactant Ion Pairs in Aqueous Solutions: Deprotonation of Dye in Ion Pair Micelles," Journal of Colloid and Interface Science, 323(2), 395-402(2008). https://doi.org/10.1016/j.jcis.2008.02.063
  23. Suzuki, Y., "Guidance for Selecting the Measurement Conditions in the Dyebinding Method for Determining Serum Protein: Theoretical Analysis Based on the Chemical Equilibrium of Protein Error," Analytical Sciences, 17(11), 1263-1268(2001). https://doi.org/10.2116/analsci.17.1263
  24. Lee, C.-S., Lee, S.-H., Kim, Y.-G., Choi, C.-H., Kim, Y.-K. and Kim, B.-G., "Biochemical Reactions on a Microfluidic Chip Based on a Precise Fluidic Handling Method at the Nanoliter Scale," Biotechnology and Bioprocess Engineering, 11(2), 146(2006). https://doi.org/10.1007/BF02931899
  25. Gyure, W. L., "Comparison of Several Methods for Semiquantitative Determination of Urinary Protein," Clinical Chemistry, 23(5), 876-879(1977). https://doi.org/10.1093/clinchem/23.5.876
  26. Jeong, S.-G., Lee, S.-H. and Lee, C.-S., "Diagnostic Paper Chip for Reliable Quantitative Detection of Albumin Using Retention Factor," KSBB Journal, 28(4), 254-259(2013). https://doi.org/10.7841/ksbbj.2013.28.4.254
  27. Hwang, C., Jeong, S.-G., Park, H.-K., Lee, C.-S. and Kim, Y.-G., "Based Neuraminidase Assay Sensor for Detection of Influenza Viruses," Korean Chemical Engineering Research, 54(3), 380-386(2016). https://doi.org/10.9713/kcer.2016.54.3.380
  28. Jeong, S.-G., Lee, S.-H., Choi, C.-H., Kim, J. and Lee, C.-S. "Toward Instrument-free Digital Measurements: a Three-dimensional Microfluidic Device Fabricated in a Single Sheet of Paper by Double-sided Printing and Lamination," Lab on a Chip, 15(4), 1188-1194(2015). https://doi.org/10.1039/C4LC01382D
  29. Washburn, E. W., "Note on a Method of Determining the Distribution of Pore Sizes in a Porous Material," Proceedings of the National Academy of Sciences of the United States of America, 7(4), 115(1921). https://doi.org/10.1073/pnas.7.4.115
  30. Carrilho, E., Martinez, A. W. and Whitesides, G. M., "Understanding Wax Printing: a Simple Micropatterning Process for Paperbased Microfluidics," Analytical Chemistry, 81(16), 7091-7095(2009). https://doi.org/10.1021/ac901071p
  31. Compton, S. J. and Jones, C. G., "Mechanism of Dye Response and Interference in the Bradford Protein Assay," Analytical Biochemistry, 151(2), 369-374(1985). https://doi.org/10.1016/0003-2697(85)90190-3
  32. Cao, W., Jiao, Q., Fu, Y., Chen, L. and Liu, Q., "Mechanism of the Interaction Between Bromophenol Blue and Bovine Serum Albumin," Spectroscopy Letters - Spectrosc Lett, 36, 197-209(2003). https://doi.org/10.1081/SL-120024351
  33. Kong, T., You, J. B., Zhang, B., Nguyen, B., Tarlan, F., Jarvi, K. and Sinton, D., "Accessory-free Quantitative Smartphone Imaging of Colorimetric Paper-based Assays," Lab on a Chip, 19(11), 1991-1999(2019). https://doi.org/10.1039/C9LC00165D
  34. Morbioli, G. G., Mazzu-Nascimento, T., Stockton, A. M. and Carrilho, E., "Technical Aspects and Challenges of Colorimetric Detection with Microfluidic Paper-based Analytical Devices (${\mu}PADs$) - A Review," Analytica Chimica Acta, 970, 1-22(2017). https://doi.org/10.1016/j.aca.2017.03.037
  35. Kim, S., Kim, D. and Kim, S., "A Rapid Real-time Quantification in Hybrid Paper-polymer Centrifugal Optical Devices," Biosensors and Bioelectronics, 126, 200-206(2019). https://doi.org/10.1016/j.bios.2018.10.064