Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Stereoselective Microbial Hydroxylation of Progestin, Norethisterone by Using Aspergillus niger and Penicillium citrinum

  • Azizuddin, Azizuddin (Department of Chemistry, Federal Urdu University of Arts, Science & Technology) ;
  • Iqbal, Muhammad (Department of Chemistry, Federal Urdu University of Arts, Science & Technology) ;
  • Musharraf, Syed Ghulam (H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi) ;
  • Shahzad, Saleem (Department of Agriculture & Agribusiness Management, University of Karachi)
  • Received : 2020.07.30
  • Accepted : 2020.09.28
  • Published : 2020.12.31
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Abstract

Microbial transformation of a potent progestin, norethisterone (17��-hydroxy-19-nor-17α-pregn-4-en-20-yn-3-one) (1) was carried out by using two filamentous fungi Aspergillus niger and Penicillium citrinum. Biotransformation of 1 with A. niger yielded a hydroxylated transformed product 10��,17��-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (2) whereas 11��,17��-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (3) was obtained through microbial transformation of 1 by P. citrinum. It is the first report of their production from 1 by using A. niger and P. citrinum with complete 1H- and 13C-NMR assignment. The structures of both metabolites were characterized by various spectroscopic techniques and reported data.

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References

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