Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Analysis and stability test of the extracts from Ulmus parvifolia leaves

참느릅나무(Ulmus parvifolia) 잎 추출물 분석 및 안정성 평가

  • Kim, Doo-Young (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Song, Soobin (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Iljoo (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Se-Kyoo (Research Division, Incospharm Corporation) ;
  • Kim, Sungwoo (Research Division, Incospharm Corporation) ;
  • Kim, Jung-Hee (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jang, Hyun-Jae (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Sei-Ryang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2020.10.28
  • Accepted : 2020.11.06
  • Published : 2020.12.31
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Abstract

A simple and reliable HPLC method was developed to determine pharmacologically standard marker compounds of Ulmus parvifolia leaves. Standard markers were characterized with neochlorogenic acid (trans-5-O-caffeoylquinic acid, 5-CQA) and chlorogenic acid (trans-3-O-caffeoylquinic acid, 3-CQA) using NMR and UPLC-QTof-MS analysis. A method for qualitative/quantitative analysis of the leaves extracts were evaluated including two compounds by using HPLC. The stability test of 30% ethanolic extracts of the leaves sample and standard markers have been evaluated for six months. However, no significant changes in the content of the marker compounds of each extract was observed during the time of investigation.

Ulmus parvifolia 잎의 약리학적 지표 성분을 제시하기 위해 간단하고 신뢰할 수 있는 HPLC 분석방법을 개발하였다. 지표 성분들은 NMR 및 UPLC-QTof-MS 분석에 의해 neochlorogenic acid (trans-5-O-caffeoylquinic acid, 5-CQA)과 chlorogenic acid (trans-3-O-caffeoylquinic acid, 3-CQA)로 구조 동정 되었다. HPLC를 이용하여 두개 화합물을 포함한 잎 추출물의 정성/정량 분석 방법을 평가하였다. 잎 30% 에탄올 추출물과 지표 성분의 안정성 테스트는 6개월 동안 평가되었다. 그러나 안정성 조사 기간 동안 추출물과 지표 성분들 각각의 함량에 대한 유의한 변화는 관찰되지 않았다.

Keywords

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