한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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식용 제비집으로부터 비극성 비드기술을 활용한 시알산의 분리정제방법에 관한 연구

A Study on Purification Process of Sialic Acid from Edible Bird's Nest Using Affinity Bead Technology

  • 김동명 ((주)케이제이엠바이오 바이오연구소) ;
  • 정주영 ((주)케이제이엠바이오 바이오연구소) ;
  • 이형곤 ((주)케이제이엠바이오 바이오연구소) ;
  • 권용성 ((주)케이제이엠바이오) ;
  • 백진홍 ((주)김정문알로에) ;
  • 한인석 (유타대학교 공과대학 화학공학과)
  • Kim, Dong-Myong (Biotechnology Research Institute, KJM Bio Ltd) ;
  • Jung, Ju-Yeong (Biotechnology Research Institute, KJM Bio Ltd) ;
  • Lee, Hyung-Kon (Biotechnology Research Institute, KJM Bio Ltd) ;
  • Kwon, Yong-Sung (Biotechnology Research Institute, KJM Bio Ltd) ;
  • Baek, Jin-Hong (R&D Center of Aloe, Kim Jung Moon Aloe Ltd) ;
  • Han, In-Suk (Department of Chemical Engineering, College of Engineering, The University of Utah)
  • 투고 : 2020.10.06
  • 심사 : 2020.12.01
  • 발행 : 2020.12.31
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초록

Sialic acid, which is contained in about 60-160 mg/kg in the edible bird's nest (EBN), is known to facilitate in the proper formation of synapses and improve memory function. The objective of this study is to extract effectively the sialic acid from edible bird's nest using affinity bead technology (ABT). After preparing the non-polar polymeric bead "KJM-278-28A" having a porous network structure, and then desorbed sialic acid was concentrated and dried. The analysis of the physicochemical properties of bead "KJM-278-28A" showed that the particle size was 400-700 ㎛, the moisture holding capacity was 67-70%, the surface area (BET) was 705-900 ㎡/g, and the average pore diameter 70-87 Å. The adsorption capacity of the bead "KJM-278-28A" for sialic acid was shown a strong physical force to bind sialic acid to the bead surface of 400 mg/L. In addition, as a result of analyzing the adsorption and desorption effects of sialic acid on water, ethanol, and 10% ethanol on the bead, it was confirmed that desorption effectively occurs from the beads when only ethanol is used. As a result of HPLC measurement of the separated sialic acid solution, a total of four sialic acid peaks of N-acetyl-neuraminic acid (Neu5Ac), α,β-anomer of Neu5Ac and N-glycoly-neuraminic acid were identified. Through these results, it was confirmed that it is possible to separate sialic acid from EBN extract with efficient and high yield when using ABT.

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