Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Isolation Characteristics of causative agent of Streptococcosis and Serotype Changes of Streptococcus parauberis from olive flounder (Paralichthys olivaceus) in Jeju

제주지역 넙치(Paralichthys olivaceus) 연쇄구균병 원인체의 분리특성과 Streptococcus parauberis의 혈청형 변화

  • Kim, Kyeong Wook (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute) ;
  • Yoo, Eun Ho (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute) ;
  • Yang, Hye Young (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute) ;
  • Kang, Bong Jo (Jeju Special Self-Governing Province Ocean and Fisheries Research Institute)
  • 김경욱 (제주특별자치도 해양수산연구원) ;
  • 유은호 (제주특별자치도 해양수산연구원) ;
  • 양혜영 (제주특별자치도 해양수산연구원) ;
  • 강봉조 (제주특별자치도 해양수산연구원)
  • Received : 2020.11.06
  • Accepted : 2020.12.01
  • Published : 2020.12.31
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Abstract

During the period from 2003 to 2020, a total of 470 Streptococcus species were isolated from farmed flounder in Jeju. Out of 470 isolates, 92 (19.6%) isolates and 378 isolates (80.4%) were identified as Streptococcus iniae and S. parauberis, respectively by multiplex PCR assay. During that period, the percentage of S. iniae decreased from 56.9% in 2003 to 0.0% in 2020 whereas that of S. parauberis increased from 43.1% in 2003 to 100% in 2020. In the PCR assay for serotyping, the isolated S. parauberis showed 3 subserotypes, Ia (34.9%), Ib/Ic (46.3%) and II (18.8%). In 2003 and 2004, serotype II was dominant at 59.1% and 50.0% of isolation rates, however between 2005 and 2009, subserotype Ib/Ic was dominant (57.6%, 86.0%, 84.6%, 57.9%, and 83.3%). After 2010, except for 2015, subserotype Ia was the most dominant one. In the last 3 years (2018 to 2020), subserotype Ia was most abundant (70%), followed by subserotype Ib/Ic (16-30%). Serotype II was not isolated in 2018, but in 2019 and 2020, it showed an increased tendency to 3.4% and 16.7%, respectively. It is believed that continuous monitoring is necessary for research on counter-measures against streptococcosis of flounder.

2013년부터 2020년까지 제주지역 양식 넙치로 부터 연쇄구균 470 균주를 분리하였다. 분리된 균주를 대상으로 multiplex PCR방법을 이용한 종 동정결과 S. iniae가 92균주(19.6%), S. parauberis가 378균주(80.4%)로 조사되었다. 연도별 경향을 보면 2003년에는 S. iniae와 S. parauberis 분리비율이 각각 56.9%와 43.1%로 조사되었으나 이후 지속적으로 S. iniae의 분리율이 감소하고 S. parauberis 분리율이 증가하기 시작하여 2015년부터는 전체 분리주가 모두 S. parauberis로 확인되었다. S. parauberis로 동정된 균주들에 대하여 PCR을 통한 serotype 분석 결과 subserotype Ia 34.9%, subserotype Ib/Ic 46.3%, serotype II 18.8% 순으로 조사되었다. S. parauberis에 대한 serotype 분포변화 조사 결과에서는 2003년과 2004년에는 serotype II가 각각 59.1%, 50.0%로 가장 많았으나, 2005년부터 2009년 사이에는 subserotype Ib/Ic가 가장 많았으며(각각 57.6%, 86.0%, 84.6%, 57.9%, 83.3%), 2010년부터 이후에는 2015년을 제외하고는 subserotype Ia가 가장 높은 비율로 분리되었고, 최근 3년(2018년부터 2020년)동안을 보면 subserotype Ia가 가장 많은 약 70% 내외이며, subserotype Ib/Ic가 약 16~30%, serotype II는 2018년에는 분리되지 않았으나 2019년과 2020년에 각각 3.4%와 16.7%로 다시 증가하는 경향을 보였다. 넙치의 연쇄구균병은 성어에 발생하여 큰 경제적 피해를 유발하므로 이에 대한 대책연구를 위해서는 지속적인 모니터링 연구가 필요하다고 생각된다.

Keywords

References

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