Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Investigation of the association of idiopathic male infertility with polymorphisms in the methionine synthase (MTR) gene

  • Tanoomand, Asghar (Department of Basic Sciences, Faculty of Medicine, Maragheh University of Medical Sciences) ;
  • Hajibemani, Abolfazl (Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz) ;
  • Abouhamzeh, Beheshteh (Department of Anatomical Sciences, Faculty of Medicine, AJA University of Medical Sciences)
  • Received : 2018.11.16
  • Accepted : 2019.05.29
  • Published : 2019.09.30
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Abstract

Objective: Spermatogenesis is a complex process that is regulated by a number of genes, some of which are involved in folate-dependent 1-carbon metabolism. Methionine synthase (encoded by MTR) is a key enzyme participating in this pathway. This study aimed to investigate the relationship of the MTR 2756A > G polymorphism with idiopathic male fertility in the Iranian population. Methods: The participants of this study included 100 men with idiopathic infertility and 100 healthy men as the control group. Genotyping of MTR 2756A > G was performed using the polymerase chain reaction and restriction fragment length polymorphism technique. The obtained data were analyzed using SPSS ver. 20.0 with a level of confidence of p< 0.05. Results: The frequencies of the A and G alleles at this locus were 77% and 23% in infertile patients and 84% and 16% in the control group, respectively. The frequencies of the GG, GA, and AA genotypes were 5%, 36%, and 59% in the infertile patients versus 3%, 27%, and 70% in the control group, respectively. No significant difference was observed in any genetic models. Conclusion: In general, the findings of this study suggest that the MTR 2756A > G single-nucleotide polymorphism is not a predisposing factor for idiopathic infertility in men.

Keywords

Acknowledgement

Supported by : AJA University of Medical Sciences of Iran

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